Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid
BACKGROUND: Marine microalga Nannochloropsis is a promising source for the production of renewable and sustainable biodiesel in replacement of depleting petroleum. Other than biodiesel, Nannochloropsis is a green and potential resource for the commercial production of nutraceutical eicosapentaenoic...
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ftpubmed:oai:pubmedcentral.nih.gov:6449970 2023-05-15T13:51:43+02:00 Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid He, Yongjin Wang, Xiaofei Wei, Hehong Zhang, Jianzhi Chen, Bilian Chen, Feng 2019-04-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449970/ http://www.ncbi.nlm.nih.gov/pubmed/30992715 https://doi.org/10.1186/s13068-019-1418-7 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449970/ http://www.ncbi.nlm.nih.gov/pubmed/30992715 http://dx.doi.org/10.1186/s13068-019-1418-7 © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Research Text 2019 ftpubmed https://doi.org/10.1186/s13068-019-1418-7 2019-04-21T00:25:03Z BACKGROUND: Marine microalga Nannochloropsis is a promising source for the production of renewable and sustainable biodiesel in replacement of depleting petroleum. Other than biodiesel, Nannochloropsis is a green and potential resource for the commercial production of nutraceutical eicosapentaenoic acid (EPA, C(20:5)). In recent studies, low-value biodiesel can be achieved by transesterification of Nannochloropsis biomass. However, it is undoubtedly wasteful to produce microalgal biodiesel containing EPA from nutritional and economical aspects. A new strategy was addressed and exploited to produce low-value bulky biodiesel along with EPA enrichment via enzymatic ethanolysis of Nannochloropsis biomass with a specific lipase. RESULTS: Cellulase pretreatment on Nannochloropsis sp. biomass significantly improved the biodiesel conversion by direct ethanolysis with five enzymes from Candida antarctica (CALA and CALB), Thermomyces lanuginosus (TL), Rhizomucor miehei (RM), and Aspergillus oryzae (PLA). Among these five biocatalysts, CALA was the best suitable enzyme to yield high biodiesel conversion and effectively enrich EPA. After optimization, the maximum biodiesel conversion (46.53–48.57%) was attained by CALA at 8:1 ethanol/biomass ratio (v/w) in 10–15% water content with 10% lipase weight at 35 °C for 72 h. Meanwhile, EPA (60.81%) was highly enriched in microalgae NPLs (neutral lipids and polar lipids), increasing original EPA levels by 1.51-fold. Moreover, this process was re-evaluated with two Nannochloropsis species (IMET1 and Salina 537). Under the optimized conditions, the biodiesel conversions of IMET1 and Salina 537 by CALA were 63.41% and 54.33%, respectively. EPA contents of microalgal NPLs were 50.06% for IMET1 and 53.73% for Salina 537. CONCLUSION: CALA was the potential biocatalyst to discriminate against EPA in the ethanolysis of Nannochloropsis biomass. The biodiesel conversion and EPA enrich efficiency of CALA were greatly dependent on lipidic class and fatty acid compositions of Nannochloropsis ... Text Antarc* Antarctica PubMed Central (PMC) Biotechnology for Biofuels 12 1 |
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Research He, Yongjin Wang, Xiaofei Wei, Hehong Zhang, Jianzhi Chen, Bilian Chen, Feng Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid |
topic_facet |
Research |
description |
BACKGROUND: Marine microalga Nannochloropsis is a promising source for the production of renewable and sustainable biodiesel in replacement of depleting petroleum. Other than biodiesel, Nannochloropsis is a green and potential resource for the commercial production of nutraceutical eicosapentaenoic acid (EPA, C(20:5)). In recent studies, low-value biodiesel can be achieved by transesterification of Nannochloropsis biomass. However, it is undoubtedly wasteful to produce microalgal biodiesel containing EPA from nutritional and economical aspects. A new strategy was addressed and exploited to produce low-value bulky biodiesel along with EPA enrichment via enzymatic ethanolysis of Nannochloropsis biomass with a specific lipase. RESULTS: Cellulase pretreatment on Nannochloropsis sp. biomass significantly improved the biodiesel conversion by direct ethanolysis with five enzymes from Candida antarctica (CALA and CALB), Thermomyces lanuginosus (TL), Rhizomucor miehei (RM), and Aspergillus oryzae (PLA). Among these five biocatalysts, CALA was the best suitable enzyme to yield high biodiesel conversion and effectively enrich EPA. After optimization, the maximum biodiesel conversion (46.53–48.57%) was attained by CALA at 8:1 ethanol/biomass ratio (v/w) in 10–15% water content with 10% lipase weight at 35 °C for 72 h. Meanwhile, EPA (60.81%) was highly enriched in microalgae NPLs (neutral lipids and polar lipids), increasing original EPA levels by 1.51-fold. Moreover, this process was re-evaluated with two Nannochloropsis species (IMET1 and Salina 537). Under the optimized conditions, the biodiesel conversions of IMET1 and Salina 537 by CALA were 63.41% and 54.33%, respectively. EPA contents of microalgal NPLs were 50.06% for IMET1 and 53.73% for Salina 537. CONCLUSION: CALA was the potential biocatalyst to discriminate against EPA in the ethanolysis of Nannochloropsis biomass. The biodiesel conversion and EPA enrich efficiency of CALA were greatly dependent on lipidic class and fatty acid compositions of Nannochloropsis ... |
format |
Text |
author |
He, Yongjin Wang, Xiaofei Wei, Hehong Zhang, Jianzhi Chen, Bilian Chen, Feng |
author_facet |
He, Yongjin Wang, Xiaofei Wei, Hehong Zhang, Jianzhi Chen, Bilian Chen, Feng |
author_sort |
He, Yongjin |
title |
Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid |
title_short |
Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid |
title_full |
Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid |
title_fullStr |
Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid |
title_full_unstemmed |
Direct enzymatic ethanolysis of potential Nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid |
title_sort |
direct enzymatic ethanolysis of potential nannochloropsis biomass for co-production of sustainable biodiesel and nutraceutical eicosapentaenoic acid |
publisher |
BioMed Central |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449970/ http://www.ncbi.nlm.nih.gov/pubmed/30992715 https://doi.org/10.1186/s13068-019-1418-7 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449970/ http://www.ncbi.nlm.nih.gov/pubmed/30992715 http://dx.doi.org/10.1186/s13068-019-1418-7 |
op_rights |
© The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
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CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s13068-019-1418-7 |
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Biotechnology for Biofuels |
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