Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic
Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea‐ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochem...
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ftpubmed:oai:pubmedcentral.nih.gov:6436437 2023-05-15T13:34:38+02:00 Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic Kan, Guangfeng Wang, Xiaofei Jiang, Jie Zhang, Chengsheng Chi, Minglei Ju, Yun Shi, Cuijuan 2018-06-21 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436437/ http://www.ncbi.nlm.nih.gov/pubmed/29926536 https://doi.org/10.1002/mbo3.657 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436437/ http://www.ncbi.nlm.nih.gov/pubmed/29926536 http://dx.doi.org/10.1002/mbo3.657 © 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Original Articles Text 2018 ftpubmed https://doi.org/10.1002/mbo3.657 2019-04-14T00:16:13Z Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea‐ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochemical characterization indicated that this yeast strain belonged to Rhodotorula mucilaginosa AN5. Heavy metal resistance pattern of Cd > Pb = Mn > Cu > Cr > Hg was observed. Scanning electron microscopic (SEM) results exhibited altered cell surface morphology under the influence of copper metal compared to that with control. The determination of physiological and biochemical changes manifested that progressive copper treatment significantly increased antioxidative reagents content and enzymes activity in the red yeast, which quench the active oxygen species to maintain the intercellular balance of redox state and ensure the cellular fission and growth. Comparative proteomic analysis revealed that, under 2 mM copper stress, 95 protein spots were tested reproducible changes of at least 10‐fold in cells. Among 95 protein spots, 43 were elevated and 52 were decreased synthesis. After MALDI TOF MS/MS analysis, 51 differentially expressed proteins were identified successfully and classified into six functional groups, including carbohydrate and energy metabolism, nucleotide and protein metabolism, protein folding, antioxidant system, signaling, and unknown function proteins. Function analysis indicated that carbohydrate and energy metabolism‐, nucleotide and protein metabolism‐, and protein folding‐related proteins played central role to the heavy metal resistance of Antarctic yeast. Generally, the results revealed that the yeast has a great capability to cope with heavy metal stress and activate the physiological and protein mechanisms, which allow more efficient recovery after copper stress. Our studies increase understanding of the molecular resistance mechanism of polar yeast to heavy metal, which will be ... Text Antarc* Antarctic Sea ice PubMed Central (PMC) Antarctic MicrobiologyOpen 8 3 e00657 |
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Original Articles |
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Original Articles Kan, Guangfeng Wang, Xiaofei Jiang, Jie Zhang, Chengsheng Chi, Minglei Ju, Yun Shi, Cuijuan Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic |
topic_facet |
Original Articles |
description |
Heavy metal pollution in Antarctic is serious by anthropogenic emissions and atmospheric transport. To dissect the heavy metal adaptation mechanisms of sea‐ice organisms, a basidiomycetous yeast strain AN5 was isolated and its cellular changes were analyzed. Morphological, physiological, and biochemical characterization indicated that this yeast strain belonged to Rhodotorula mucilaginosa AN5. Heavy metal resistance pattern of Cd > Pb = Mn > Cu > Cr > Hg was observed. Scanning electron microscopic (SEM) results exhibited altered cell surface morphology under the influence of copper metal compared to that with control. The determination of physiological and biochemical changes manifested that progressive copper treatment significantly increased antioxidative reagents content and enzymes activity in the red yeast, which quench the active oxygen species to maintain the intercellular balance of redox state and ensure the cellular fission and growth. Comparative proteomic analysis revealed that, under 2 mM copper stress, 95 protein spots were tested reproducible changes of at least 10‐fold in cells. Among 95 protein spots, 43 were elevated and 52 were decreased synthesis. After MALDI TOF MS/MS analysis, 51 differentially expressed proteins were identified successfully and classified into six functional groups, including carbohydrate and energy metabolism, nucleotide and protein metabolism, protein folding, antioxidant system, signaling, and unknown function proteins. Function analysis indicated that carbohydrate and energy metabolism‐, nucleotide and protein metabolism‐, and protein folding‐related proteins played central role to the heavy metal resistance of Antarctic yeast. Generally, the results revealed that the yeast has a great capability to cope with heavy metal stress and activate the physiological and protein mechanisms, which allow more efficient recovery after copper stress. Our studies increase understanding of the molecular resistance mechanism of polar yeast to heavy metal, which will be ... |
format |
Text |
author |
Kan, Guangfeng Wang, Xiaofei Jiang, Jie Zhang, Chengsheng Chi, Minglei Ju, Yun Shi, Cuijuan |
author_facet |
Kan, Guangfeng Wang, Xiaofei Jiang, Jie Zhang, Chengsheng Chi, Minglei Ju, Yun Shi, Cuijuan |
author_sort |
Kan, Guangfeng |
title |
Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic |
title_short |
Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic |
title_full |
Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic |
title_fullStr |
Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic |
title_full_unstemmed |
Copper stress response in yeast Rhodotorula mucilaginosa AN5 isolated from sea ice, Antarctic |
title_sort |
copper stress response in yeast rhodotorula mucilaginosa an5 isolated from sea ice, antarctic |
publisher |
John Wiley and Sons Inc. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436437/ http://www.ncbi.nlm.nih.gov/pubmed/29926536 https://doi.org/10.1002/mbo3.657 |
geographic |
Antarctic |
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Antarctic |
genre |
Antarc* Antarctic Sea ice |
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Antarc* Antarctic Sea ice |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436437/ http://www.ncbi.nlm.nih.gov/pubmed/29926536 http://dx.doi.org/10.1002/mbo3.657 |
op_rights |
© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
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CC-BY |
op_doi |
https://doi.org/10.1002/mbo3.657 |
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MicrobiologyOpen |
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e00657 |
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