Urea dilution of serum for reproducible anti-HSV1 IgG avidity index
BACKGROUND: Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content...
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ftpubmed:oai:pubmedcentral.nih.gov:6376645 2023-05-15T17:44:46+02:00 Urea dilution of serum for reproducible anti-HSV1 IgG avidity index Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik 2019-02-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376645/ http://www.ncbi.nlm.nih.gov/pubmed/30764767 https://doi.org/10.1186/s12879-019-3769-x en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376645/ http://www.ncbi.nlm.nih.gov/pubmed/30764767 http://dx.doi.org/10.1186/s12879-019-3769-x © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. CC0 PDM CC-BY Technical Advance Text 2019 ftpubmed https://doi.org/10.1186/s12879-019-3769-x 2019-03-03T01:27:06Z BACKGROUND: Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. METHODS: Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. RESULTS: AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900–0.979) and 0.989 (95% CI 0.969–0.996) in the biobank and clinical samples, respectively. CONCLUSION: The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts. Text Northern Sweden PubMed Central (PMC) BMC Infectious Diseases 19 1 |
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Technical Advance Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
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Technical Advance |
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BACKGROUND: Herpes simplex virus type 1 (HSV1), establishes life-long latency and can cause symptoms during both first-time infection and later reactivation. The aim of the present study was to describe a protocol to generate a reliable and discriminative avidity index (AI) for anti-HSV1 IgG content in human sera. METHODS: Human serum from two distinct cohorts; one a biobank collection (Betula) (n = 28), and one from a clinical diagnostics laboratory at Northern Sweden University Hospital (NUS) (n = 18), were assessed for presence of IgG antibodies against HSV1 by a commercially available ELISA-kit. Addition of urea at the incubation step reduces effective binding, and the ratio between urea treated sample and non-treated sample was used to express an avidity index (AI) for individual samples. RESULTS: AI score ranged between 43.2 and 73.4% among anti-HSV1 positive biobank sera. Clinical samples ranged between 36.3 and 74.9%. Reproducibility expressed as an intraclass correlation coefficient (ICC) was estimated at 0.948 (95% CI: 0.900–0.979) and 0.989 (95% CI 0.969–0.996) in the biobank and clinical samples, respectively. CONCLUSION: The method allows for AI scoring of anti-HSV1 IgG from individual human sera with a single measurement. The least significant change between two measurements at the p < 0.05 level was estimated at 5.4 and 3.2 points, respectively, for the two assessed cohorts. |
format |
Text |
author |
Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik |
author_facet |
Olsson, Jan Johansson, Jörgen Honkala, Emma Blomqvist, Bert Kok, Eloise Weidung, Bodil Lövheim, Hugo Elgh, Fredrik |
author_sort |
Olsson, Jan |
title |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_short |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_full |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_fullStr |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_full_unstemmed |
Urea dilution of serum for reproducible anti-HSV1 IgG avidity index |
title_sort |
urea dilution of serum for reproducible anti-hsv1 igg avidity index |
publisher |
BioMed Central |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376645/ http://www.ncbi.nlm.nih.gov/pubmed/30764767 https://doi.org/10.1186/s12879-019-3769-x |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376645/ http://www.ncbi.nlm.nih.gov/pubmed/30764767 http://dx.doi.org/10.1186/s12879-019-3769-x |
op_rights |
© The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
op_rightsnorm |
CC0 PDM CC-BY |
op_doi |
https://doi.org/10.1186/s12879-019-3769-x |
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BMC Infectious Diseases |
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19 |
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