Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida
Aeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys...
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ftpubmed:oai:pubmedcentral.nih.gov:6376026 2023-05-15T15:28:27+02:00 Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Yang, Dong Liu, Ying 2019-02-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376026/ http://www.ncbi.nlm.nih.gov/pubmed/30765835 https://doi.org/10.1038/s41598-019-38890-3 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376026/ http://www.ncbi.nlm.nih.gov/pubmed/30765835 http://dx.doi.org/10.1038/s41598-019-38890-3 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-019-38890-3 2019-02-24T01:18:08Z Aeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. salmonicida. Phosphoproteomic analyses were conducted to further clarify the dynamic changes in protein phosphorylation patterns triggered by bacterial infection. To our knowledge, this is the first study to characterize phosphorylation events in proteins from A. salmonicida-infected Atlantic salmon. Overall, we identified over 5635 phosphorylation sites in 3112 proteins, and 1502 up-regulated and 77 down-regulated proteins quantified as a 1.5-fold or greater change relative to control levels. Based on the combined data from proteomic and motif analyses, we hypothesize that five prospective novel kinases (VRK3, GAK, HCK, PKCδ and RSK6) with common functions in inflammatory processes and cellular pathways to regulate apoptosis and the cytoskeleton could serve as potential biomarkers against bacterial propagation in fish. Data from STRING-based functional network analyses indicate that fga is the most central protein. Our collective findings provide new insights into protein phosphorylation patterns, which may serve as effective indicators of A. salmonicida infection in Atlantic salmon. Text Atlantic salmon Salmo salar PubMed Central (PMC) Scientific Reports 9 1 |
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Article Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Yang, Dong Liu, Ying Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida |
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Aeromonas salmonicida (A. salmonicida) is a pathogenic bacterium that causes furunculosis and poses a significant global risk, particularly in economic activities such as Atlantic salmon (Salmo salar) farming. In a previous study, we identified proteins that are significantly upregulated in kidneys of Atlantic salmon challenged with A. salmonicida. Phosphoproteomic analyses were conducted to further clarify the dynamic changes in protein phosphorylation patterns triggered by bacterial infection. To our knowledge, this is the first study to characterize phosphorylation events in proteins from A. salmonicida-infected Atlantic salmon. Overall, we identified over 5635 phosphorylation sites in 3112 proteins, and 1502 up-regulated and 77 down-regulated proteins quantified as a 1.5-fold or greater change relative to control levels. Based on the combined data from proteomic and motif analyses, we hypothesize that five prospective novel kinases (VRK3, GAK, HCK, PKCδ and RSK6) with common functions in inflammatory processes and cellular pathways to regulate apoptosis and the cytoskeleton could serve as potential biomarkers against bacterial propagation in fish. Data from STRING-based functional network analyses indicate that fga is the most central protein. Our collective findings provide new insights into protein phosphorylation patterns, which may serve as effective indicators of A. salmonicida infection in Atlantic salmon. |
format |
Text |
author |
Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Yang, Dong Liu, Ying |
author_facet |
Liu, Peng-fei Du, Yishuai Meng, Lingjie Li, Xian Yang, Dong Liu, Ying |
author_sort |
Liu, Peng-fei |
title |
Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida |
title_short |
Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida |
title_full |
Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida |
title_fullStr |
Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida |
title_full_unstemmed |
Phosphoproteomic analyses of kidneys of Atlantic salmon infected with Aeromonas salmonicida |
title_sort |
phosphoproteomic analyses of kidneys of atlantic salmon infected with aeromonas salmonicida |
publisher |
Nature Publishing Group UK |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376026/ http://www.ncbi.nlm.nih.gov/pubmed/30765835 https://doi.org/10.1038/s41598-019-38890-3 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6376026/ http://www.ncbi.nlm.nih.gov/pubmed/30765835 http://dx.doi.org/10.1038/s41598-019-38890-3 |
op_rights |
© The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-38890-3 |
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