Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr
With respect to salmonid aquaculture, one of the most important bacterial pathogens due to high mortality and antibiotic usage is the causative agent of typical furunculosis, Aeromonas salmonicida spp. salmonicida (Asal). In Atlantic salmon, Salmo salar, the host response during infections with Asal...
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ftpubmed:oai:pubmedcentral.nih.gov:6369366 2023-05-15T14:30:03+02:00 Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr Braden, Laura M. Whyte, Shona K. Brown, Alyson B. J. Iderstine, Carter Van Letendre, Corinne Groman, David Lewis, Jeff Purcell, Sara L. Hori, Tiago Fast, Mark D. 2019-02-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369366/ http://www.ncbi.nlm.nih.gov/pubmed/30778356 https://doi.org/10.3389/fimmu.2019.00120 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369366/ http://www.ncbi.nlm.nih.gov/pubmed/30778356 http://dx.doi.org/10.3389/fimmu.2019.00120 Copyright © 2019 Braden, Whyte, Brown, Van Iderstine, Letendre, Groman, Lewis, Purcell, Hori and Fast. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Immunology Text 2019 ftpubmed https://doi.org/10.3389/fimmu.2019.00120 2019-02-24T01:13:20Z With respect to salmonid aquaculture, one of the most important bacterial pathogens due to high mortality and antibiotic usage is the causative agent of typical furunculosis, Aeromonas salmonicida spp. salmonicida (Asal). In Atlantic salmon, Salmo salar, the host response during infections with Asal is well-documented, with furunculosis outbreaks resulting in significant mortality in commercial settings. However, less is known about the host-pathogen interactions in the emerging aquaculture species, Arctic charr Salvelinus alpinus. Furthermore, there is no data on the efficacy or response of this species after vaccination with commonly administered vaccines against furunculosis. To this end, we examined the immunological response of S. alpinus during infection with Asal, with or without administration of vaccines (Forte Micro®, Forte Micro® + Renogen®, Elanco Animal Health). Artic charr (vaccinated or unvaccinated) were i.p.-injected with a virulent strain of Asal (106 CFUs/mL) and tissues were collected pre-infection/post-vaccination, 8, and 29 days post-infection. Unvaccinated Arctic charr were susceptible to Asal with 72% mortalities observed after 31 days. However, there was 72–82% protection in fish vaccinated with either the single or dual-vaccine, respectively. Protection in vaccinated fish was concordant with significantly higher serum IgM concentrations, and following RNA sequencing and transcriptome assembly, differential expression analysis revealed several patterns and pathways associated with the improved survival of vaccinated fish. Most striking was the dramatically higher basal expression of complement/coagulation factors, acute phase-proteins, and iron hemostasis proteins in pre-challenged, vaccinated fish. Remarkably, following Asal infection, this response was abrogated and instead the transcriptome was characterized by a lack of immune-stimulation compared to that of unvaccinated fish. Furthermore, where pathways of actin assembly and FcγR-mediated phagocytosis were significantly ... Text Arctic charr Arctic Atlantic salmon Salmo salar Salvelinus alpinus PubMed Central (PMC) Arctic Frontiers in Immunology 10 |
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Immunology |
spellingShingle |
Immunology Braden, Laura M. Whyte, Shona K. Brown, Alyson B. J. Iderstine, Carter Van Letendre, Corinne Groman, David Lewis, Jeff Purcell, Sara L. Hori, Tiago Fast, Mark D. Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr |
topic_facet |
Immunology |
description |
With respect to salmonid aquaculture, one of the most important bacterial pathogens due to high mortality and antibiotic usage is the causative agent of typical furunculosis, Aeromonas salmonicida spp. salmonicida (Asal). In Atlantic salmon, Salmo salar, the host response during infections with Asal is well-documented, with furunculosis outbreaks resulting in significant mortality in commercial settings. However, less is known about the host-pathogen interactions in the emerging aquaculture species, Arctic charr Salvelinus alpinus. Furthermore, there is no data on the efficacy or response of this species after vaccination with commonly administered vaccines against furunculosis. To this end, we examined the immunological response of S. alpinus during infection with Asal, with or without administration of vaccines (Forte Micro®, Forte Micro® + Renogen®, Elanco Animal Health). Artic charr (vaccinated or unvaccinated) were i.p.-injected with a virulent strain of Asal (106 CFUs/mL) and tissues were collected pre-infection/post-vaccination, 8, and 29 days post-infection. Unvaccinated Arctic charr were susceptible to Asal with 72% mortalities observed after 31 days. However, there was 72–82% protection in fish vaccinated with either the single or dual-vaccine, respectively. Protection in vaccinated fish was concordant with significantly higher serum IgM concentrations, and following RNA sequencing and transcriptome assembly, differential expression analysis revealed several patterns and pathways associated with the improved survival of vaccinated fish. Most striking was the dramatically higher basal expression of complement/coagulation factors, acute phase-proteins, and iron hemostasis proteins in pre-challenged, vaccinated fish. Remarkably, following Asal infection, this response was abrogated and instead the transcriptome was characterized by a lack of immune-stimulation compared to that of unvaccinated fish. Furthermore, where pathways of actin assembly and FcγR-mediated phagocytosis were significantly ... |
format |
Text |
author |
Braden, Laura M. Whyte, Shona K. Brown, Alyson B. J. Iderstine, Carter Van Letendre, Corinne Groman, David Lewis, Jeff Purcell, Sara L. Hori, Tiago Fast, Mark D. |
author_facet |
Braden, Laura M. Whyte, Shona K. Brown, Alyson B. J. Iderstine, Carter Van Letendre, Corinne Groman, David Lewis, Jeff Purcell, Sara L. Hori, Tiago Fast, Mark D. |
author_sort |
Braden, Laura M. |
title |
Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr |
title_short |
Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr |
title_full |
Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr |
title_fullStr |
Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr |
title_full_unstemmed |
Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr |
title_sort |
vaccine-induced protection against furunculosis involves pre-emptive priming of humoral immunity in arctic charr |
publisher |
Frontiers Media S.A. |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369366/ http://www.ncbi.nlm.nih.gov/pubmed/30778356 https://doi.org/10.3389/fimmu.2019.00120 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Atlantic salmon Salmo salar Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Atlantic salmon Salmo salar Salvelinus alpinus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369366/ http://www.ncbi.nlm.nih.gov/pubmed/30778356 http://dx.doi.org/10.3389/fimmu.2019.00120 |
op_rights |
Copyright © 2019 Braden, Whyte, Brown, Van Iderstine, Letendre, Groman, Lewis, Purcell, Hori and Fast. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2019.00120 |
container_title |
Frontiers in Immunology |
container_volume |
10 |
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