In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ....
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ftpubmed:oai:pubmedcentral.nih.gov:6337334 2023-05-15T15:58:00+02:00 In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire 2019-01-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337334/ http://www.ncbi.nlm.nih.gov/pubmed/30625986 https://doi.org/10.3390/ijms20010197 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337334/ http://www.ncbi.nlm.nih.gov/pubmed/30625986 http://dx.doi.org/10.3390/ijms20010197 © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Communication Text 2019 ftpubmed https://doi.org/10.3390/ijms20010197 2019-01-27T01:37:30Z Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific International Journal of Molecular Sciences 20 1 197 |
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Communication Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
topic_facet |
Communication |
description |
Following their planktonic phase, the larvae of benthic marine organisms must locate a suitable habitat to settle and metamorphose. For oysters, larval adhesion occurs at the pediveliger stage with the secretion of a proteinaceous bioadhesive produced by the foot, a specialized and ephemeral organ. Oyster bioadhesive is highly resistant to proteomic extraction and is only produced in very low quantities, which explains why it has been very little examined in larvae to date. In silico analysis of nucleic acid databases could help to identify genes of interest implicated in settlement. In this work, the publicly available transcriptome of Pacific oyster Crassostrea gigas over its developmental stages was mined to select genes highly expressed at the pediveliger stage. Our analysis revealed 59 sequences potentially implicated in adhesion of C. gigas larvae. Some related proteins contain conserved domains already described in other bioadhesives. We propose a hypothetic composition of C. gigas bioadhesive in which the protein constituent is probably composed of collagen and the von Willebrand Factor domain could play a role in adhesive cohesion. Genes coding for enzymes implicated in DOPA chemistry were also detected, indicating that this modification is also potentially present in the adhesive of pediveliger larvae. |
format |
Text |
author |
Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire |
author_facet |
Foulon, Valentin Boudry, Pierre Artigaud, Sébastien Guérard, Fabienne Hellio, Claire |
author_sort |
Foulon, Valentin |
title |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_short |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_fullStr |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_full_unstemmed |
In Silico Analysis of Pacific Oyster (Crassostrea gigas) Transcriptome over Developmental Stages Reveals Candidate Genes for Larval Settlement |
title_sort |
in silico analysis of pacific oyster (crassostrea gigas) transcriptome over developmental stages reveals candidate genes for larval settlement |
publisher |
MDPI |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337334/ http://www.ncbi.nlm.nih.gov/pubmed/30625986 https://doi.org/10.3390/ijms20010197 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337334/ http://www.ncbi.nlm.nih.gov/pubmed/30625986 http://dx.doi.org/10.3390/ijms20010197 |
op_rights |
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/ijms20010197 |
container_title |
International Journal of Molecular Sciences |
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20 |
container_issue |
1 |
container_start_page |
197 |
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1766393725945118720 |