Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine?
The evolution of antifreeze glycoproteins has enabled notothenioid fish to flourish in the freezing waters of the Southern Ocean. Whereas successful at the biodiversity level to life in the cold, paradoxically at the cellular level these stenothermal animals have problems producing, folding, and deg...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336007/ http://www.ncbi.nlm.nih.gov/pubmed/30496401 https://doi.org/10.1093/gbe/evy262 |
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ftpubmed:oai:pubmedcentral.nih.gov:6336007 2023-05-15T13:36:40+02:00 Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S 2018-11-29 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336007/ http://www.ncbi.nlm.nih.gov/pubmed/30496401 https://doi.org/10.1093/gbe/evy262 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336007/ http://www.ncbi.nlm.nih.gov/pubmed/30496401 http://dx.doi.org/10.1093/gbe/evy262 © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2018 ftpubmed https://doi.org/10.1093/gbe/evy262 2019-01-27T01:35:52Z The evolution of antifreeze glycoproteins has enabled notothenioid fish to flourish in the freezing waters of the Southern Ocean. Whereas successful at the biodiversity level to life in the cold, paradoxically at the cellular level these stenothermal animals have problems producing, folding, and degrading proteins at their ambient temperatures of –1.86 °C. In this first multi-species transcriptome comparison of the amino acid composition of notothenioid proteins with temperate teleost proteins, we show that, unlike psychrophilic bacteria, Antarctic fish provide little evidence for the mass alteration of protein amino acid composition to enhance protein folding and reduce protein denaturation in the cold. The exception was the significant overrepresentation of positions where leucine in temperate fish proteins was replaced by methionine in the notothenioid orthologues. We hypothesize that these extra methionines have been preferentially assimilated into the genome to act as redox sensors in the highly oxygenated waters of the Southern Ocean. This redox hypothesis is supported by analyses of notothenioids showing enrichment of genes associated with responses to environmental stress, particularly reactive oxygen species. So overall, although notothenioid fish show cold-associated problems with protein homeostasis, they may have modified only a selected number of biochemical pathways to work efficiently below 0 °C. Even a slight warming of the Southern Ocean might disrupt the critical functions of this handful of key pathways with considerable impacts for the functioning of this ecosystem in the future. Text Antarc* Antarctic Southern Ocean PubMed Central (PMC) Antarctic Southern Ocean Genome Biology and Evolution 11 1 220 231 |
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Research Article Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
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Research Article |
description |
The evolution of antifreeze glycoproteins has enabled notothenioid fish to flourish in the freezing waters of the Southern Ocean. Whereas successful at the biodiversity level to life in the cold, paradoxically at the cellular level these stenothermal animals have problems producing, folding, and degrading proteins at their ambient temperatures of –1.86 °C. In this first multi-species transcriptome comparison of the amino acid composition of notothenioid proteins with temperate teleost proteins, we show that, unlike psychrophilic bacteria, Antarctic fish provide little evidence for the mass alteration of protein amino acid composition to enhance protein folding and reduce protein denaturation in the cold. The exception was the significant overrepresentation of positions where leucine in temperate fish proteins was replaced by methionine in the notothenioid orthologues. We hypothesize that these extra methionines have been preferentially assimilated into the genome to act as redox sensors in the highly oxygenated waters of the Southern Ocean. This redox hypothesis is supported by analyses of notothenioids showing enrichment of genes associated with responses to environmental stress, particularly reactive oxygen species. So overall, although notothenioid fish show cold-associated problems with protein homeostasis, they may have modified only a selected number of biochemical pathways to work efficiently below 0 °C. Even a slight warming of the Southern Ocean might disrupt the critical functions of this handful of key pathways with considerable impacts for the functioning of this ecosystem in the future. |
format |
Text |
author |
Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S |
author_facet |
Berthelot, Camille Clarke, Jane Desvignes, Thomas William Detrich, H Flicek, Paul Peck, Lloyd S Peters, Michael Postlethwait, John H Clark, Melody S |
author_sort |
Berthelot, Camille |
title |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_short |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_full |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_fullStr |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_full_unstemmed |
Adaptation of Proteins to the Cold in Antarctic Fish: A Role for Methionine? |
title_sort |
adaptation of proteins to the cold in antarctic fish: a role for methionine? |
publisher |
Oxford University Press |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336007/ http://www.ncbi.nlm.nih.gov/pubmed/30496401 https://doi.org/10.1093/gbe/evy262 |
geographic |
Antarctic Southern Ocean |
geographic_facet |
Antarctic Southern Ocean |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336007/ http://www.ncbi.nlm.nih.gov/pubmed/30496401 http://dx.doi.org/10.1093/gbe/evy262 |
op_rights |
© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1093/gbe/evy262 |
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Genome Biology and Evolution |
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11 |
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1 |
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220 |
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231 |
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1766082175532269568 |