Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod
The genetic mechanisms determining sex in teleost fishes are highly variable and the master sex determining gene has only been identified in few species. Here we characterize a male-specific region of 9 kb on linkage group 11 in Atlantic cod (Gadus morhua) harboring a single gene named zkY for zinc...
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ftpubmed:oai:pubmedcentral.nih.gov:6333804 2023-05-15T14:30:28+02:00 Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod Kirubakaran, Tina Graceline Andersen, Øivind De Rosa, Maria Cristina Andersstuen, Terese Hallan, Kristina Kent, Matthew Peter Lien, Sigbjørn 2019-01-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333804/ http://www.ncbi.nlm.nih.gov/pubmed/30644412 https://doi.org/10.1038/s41598-018-36748-8 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333804/ http://www.ncbi.nlm.nih.gov/pubmed/30644412 http://dx.doi.org/10.1038/s41598-018-36748-8 © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2019 ftpubmed https://doi.org/10.1038/s41598-018-36748-8 2019-01-20T01:26:57Z The genetic mechanisms determining sex in teleost fishes are highly variable and the master sex determining gene has only been identified in few species. Here we characterize a male-specific region of 9 kb on linkage group 11 in Atlantic cod (Gadus morhua) harboring a single gene named zkY for zinc knuckle on the Y chromosome. Diagnostic PCR test of phenotypically sexed males and females confirm the sex-specific nature of the Y-sequence. We identified twelve highly similar autosomal gene copies of zkY, of which eight code for proteins containing the zinc knuckle motif. 3D modeling suggests that the amino acid changes observed in six copies might influence the putative RNA-binding specificity. Cod zkY and the autosomal proteins zk1 and zk2 possess an identical zinc knuckle structure, but only the Y-specific gene zkY was expressed at high levels in the developing larvae before the onset of sex differentiation. Collectively these data suggest zkY as a candidate master masculinization gene in Atlantic cod. PCR amplification of Y-sequences in Arctic cod (Arctogadus glacialis) and Greenland cod (Gadus macrocephalus ogac) suggests that the male-specific region emerged in codfishes more than 7.5 million years ago. Text Arctic cod Arctic Arctogadus glacialis atlantic cod Gadus morhua Greenland Greenland cod PubMed Central (PMC) Arctic Greenland The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) Scientific Reports 9 1 |
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Article Kirubakaran, Tina Graceline Andersen, Øivind De Rosa, Maria Cristina Andersstuen, Terese Hallan, Kristina Kent, Matthew Peter Lien, Sigbjørn Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod |
topic_facet |
Article |
description |
The genetic mechanisms determining sex in teleost fishes are highly variable and the master sex determining gene has only been identified in few species. Here we characterize a male-specific region of 9 kb on linkage group 11 in Atlantic cod (Gadus morhua) harboring a single gene named zkY for zinc knuckle on the Y chromosome. Diagnostic PCR test of phenotypically sexed males and females confirm the sex-specific nature of the Y-sequence. We identified twelve highly similar autosomal gene copies of zkY, of which eight code for proteins containing the zinc knuckle motif. 3D modeling suggests that the amino acid changes observed in six copies might influence the putative RNA-binding specificity. Cod zkY and the autosomal proteins zk1 and zk2 possess an identical zinc knuckle structure, but only the Y-specific gene zkY was expressed at high levels in the developing larvae before the onset of sex differentiation. Collectively these data suggest zkY as a candidate master masculinization gene in Atlantic cod. PCR amplification of Y-sequences in Arctic cod (Arctogadus glacialis) and Greenland cod (Gadus macrocephalus ogac) suggests that the male-specific region emerged in codfishes more than 7.5 million years ago. |
format |
Text |
author |
Kirubakaran, Tina Graceline Andersen, Øivind De Rosa, Maria Cristina Andersstuen, Terese Hallan, Kristina Kent, Matthew Peter Lien, Sigbjørn |
author_facet |
Kirubakaran, Tina Graceline Andersen, Øivind De Rosa, Maria Cristina Andersstuen, Terese Hallan, Kristina Kent, Matthew Peter Lien, Sigbjørn |
author_sort |
Kirubakaran, Tina Graceline |
title |
Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod |
title_short |
Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod |
title_full |
Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod |
title_fullStr |
Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod |
title_full_unstemmed |
Characterization of a male specific region containing a candidate sex determining gene in Atlantic cod |
title_sort |
characterization of a male specific region containing a candidate sex determining gene in atlantic cod |
publisher |
Nature Publishing Group UK |
publishDate |
2019 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333804/ http://www.ncbi.nlm.nih.gov/pubmed/30644412 https://doi.org/10.1038/s41598-018-36748-8 |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
Arctic Greenland The ''Y'' |
geographic_facet |
Arctic Greenland The ''Y'' |
genre |
Arctic cod Arctic Arctogadus glacialis atlantic cod Gadus morhua Greenland Greenland cod |
genre_facet |
Arctic cod Arctic Arctogadus glacialis atlantic cod Gadus morhua Greenland Greenland cod |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333804/ http://www.ncbi.nlm.nih.gov/pubmed/30644412 http://dx.doi.org/10.1038/s41598-018-36748-8 |
op_rights |
© The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-018-36748-8 |
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Scientific Reports |
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9 |
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