Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy
In this article, an analytical methodology to investigate the proteinaceous content in atmospheric size-resolved aerosols collected at the Zeppelin observatory (79 °N, 12 °E) at Ny Ålesund, Svalbard, from September to December 2015, is proposed. Quantitative determination was performed after acidic...
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ftpubmed:oai:pubmedcentral.nih.gov:6318243 2023-05-15T15:00:47+02:00 Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy Mashayekhy Rad, Farshid Zurita, Javier Gilles, Philippe Rutgeerts, Laurens A. J. Nilsson, Ulrika Ilag, Leopold L. Leck, Caroline 2018-07-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318243/ http://www.ncbi.nlm.nih.gov/pubmed/30019163 https://doi.org/10.1007/s13361-018-2009-8 en eng Springer US http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318243/ http://www.ncbi.nlm.nih.gov/pubmed/30019163 http://dx.doi.org/10.1007/s13361-018-2009-8 © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. CC-BY Focus: Honoring Carol V. Robinson’s Election to the National Academy of Sciences: Research Article Text 2018 ftpubmed https://doi.org/10.1007/s13361-018-2009-8 2019-01-20T01:15:25Z In this article, an analytical methodology to investigate the proteinaceous content in atmospheric size-resolved aerosols collected at the Zeppelin observatory (79 °N, 12 °E) at Ny Ålesund, Svalbard, from September to December 2015, is proposed. Quantitative determination was performed after acidic hydrolysis using ultrahigh-performance liquid chromatography in reversed-phase mode coupled to electrospray ionization tandem mass spectrometry. Chromatographic separation, as well as specificity in the identification, was achieved by derivatization of the amino acids with N-butyl nicotinic acid N-hydroxysuccinimide ester prior to the analysis. The chromatographic run was performed within 11 min and instrumental levels of detection (LODs) were between 0.2 and 8.1 pg injected on the column, except for arginine which exhibited an LOD of 37 pg. Corresponding method LODs were between 0.01 and 1.9 fmol/m3, based on the average air sampling volume of 57 m3. The sum of free amino acids and hydrolyzed polyamino acids was shown to vary within 6–2914 and 0.02–1417 pmol/m3 for particles in sizes < 2 and 2–10 μm in equivalent aerodynamic diameter, respectively. Leucine, alanine, and valine were the most abundant among the amino acids in both aerosol size fractions. In an attempt to elucidate source areas of the collected aerosols, 5- to 10-day 3D backward trajectories reaching the sampling station were calculated. Overall, the method described here provides a first time estimate of the proteinaceous content, that is, the sum of free and polyamino acids, in size-resolved aerosols collected in the Arctic. Text Arctic Ny Ålesund Ny-Ålesund Svalbard PubMed Central (PMC) Arctic Ny-Ålesund Svalbard Journal of The American Society for Mass Spectrometry 30 1 161 173 |
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Open Polar |
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PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Focus: Honoring Carol V. Robinson’s Election to the National Academy of Sciences: Research Article |
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Focus: Honoring Carol V. Robinson’s Election to the National Academy of Sciences: Research Article Mashayekhy Rad, Farshid Zurita, Javier Gilles, Philippe Rutgeerts, Laurens A. J. Nilsson, Ulrika Ilag, Leopold L. Leck, Caroline Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy |
topic_facet |
Focus: Honoring Carol V. Robinson’s Election to the National Academy of Sciences: Research Article |
description |
In this article, an analytical methodology to investigate the proteinaceous content in atmospheric size-resolved aerosols collected at the Zeppelin observatory (79 °N, 12 °E) at Ny Ålesund, Svalbard, from September to December 2015, is proposed. Quantitative determination was performed after acidic hydrolysis using ultrahigh-performance liquid chromatography in reversed-phase mode coupled to electrospray ionization tandem mass spectrometry. Chromatographic separation, as well as specificity in the identification, was achieved by derivatization of the amino acids with N-butyl nicotinic acid N-hydroxysuccinimide ester prior to the analysis. The chromatographic run was performed within 11 min and instrumental levels of detection (LODs) were between 0.2 and 8.1 pg injected on the column, except for arginine which exhibited an LOD of 37 pg. Corresponding method LODs were between 0.01 and 1.9 fmol/m3, based on the average air sampling volume of 57 m3. The sum of free amino acids and hydrolyzed polyamino acids was shown to vary within 6–2914 and 0.02–1417 pmol/m3 for particles in sizes < 2 and 2–10 μm in equivalent aerodynamic diameter, respectively. Leucine, alanine, and valine were the most abundant among the amino acids in both aerosol size fractions. In an attempt to elucidate source areas of the collected aerosols, 5- to 10-day 3D backward trajectories reaching the sampling station were calculated. Overall, the method described here provides a first time estimate of the proteinaceous content, that is, the sum of free and polyamino acids, in size-resolved aerosols collected in the Arctic. |
format |
Text |
author |
Mashayekhy Rad, Farshid Zurita, Javier Gilles, Philippe Rutgeerts, Laurens A. J. Nilsson, Ulrika Ilag, Leopold L. Leck, Caroline |
author_facet |
Mashayekhy Rad, Farshid Zurita, Javier Gilles, Philippe Rutgeerts, Laurens A. J. Nilsson, Ulrika Ilag, Leopold L. Leck, Caroline |
author_sort |
Mashayekhy Rad, Farshid |
title |
Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy |
title_short |
Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy |
title_full |
Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy |
title_fullStr |
Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy |
title_full_unstemmed |
Measurements of Atmospheric Proteinaceous Aerosol in the Arctic Using a Selective UHPLC/ESI-MS/MS Strategy |
title_sort |
measurements of atmospheric proteinaceous aerosol in the arctic using a selective uhplc/esi-ms/ms strategy |
publisher |
Springer US |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318243/ http://www.ncbi.nlm.nih.gov/pubmed/30019163 https://doi.org/10.1007/s13361-018-2009-8 |
geographic |
Arctic Ny-Ålesund Svalbard |
geographic_facet |
Arctic Ny-Ålesund Svalbard |
genre |
Arctic Ny Ålesund Ny-Ålesund Svalbard |
genre_facet |
Arctic Ny Ålesund Ny-Ålesund Svalbard |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318243/ http://www.ncbi.nlm.nih.gov/pubmed/30019163 http://dx.doi.org/10.1007/s13361-018-2009-8 |
op_rights |
© The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s13361-018-2009-8 |
container_title |
Journal of The American Society for Mass Spectrometry |
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30 |
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1 |
container_start_page |
161 |
op_container_end_page |
173 |
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1766332847572910080 |