The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis
The grizzly bear (Ursus arctos ssp. horribilis) represents the largest population of brown bears in North America. Its genome was sequenced using a microfluidic partitioning library construction technique, and these data were supplemented with sequencing from a nanopore-based long read platform. The...
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ftpubmed:oai:pubmedcentral.nih.gov:6315469 2023-05-15T18:41:49+02:00 The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis Taylor, Gregory A. Kirk, Heather Coombe, Lauren Jackman, Shaun D. Chu, Justin Tse, Kane Cheng, Dean Chuah, Eric Pandoh, Pawan Carlsen, Rebecca Zhao, Yongjun Mungall, Andrew J. Moore, Richard Birol, Inanc Franke, Maria Marra, Marco A. Dutton, Christopher Jones, Steven J. M. 2018-11-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315469/ http://www.ncbi.nlm.nih.gov/pubmed/30513700 https://doi.org/10.3390/genes9120598 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315469/ http://www.ncbi.nlm.nih.gov/pubmed/30513700 http://dx.doi.org/10.3390/genes9120598 © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2018 ftpubmed https://doi.org/10.3390/genes9120598 2019-01-13T01:26:01Z The grizzly bear (Ursus arctos ssp. horribilis) represents the largest population of brown bears in North America. Its genome was sequenced using a microfluidic partitioning library construction technique, and these data were supplemented with sequencing from a nanopore-based long read platform. The final assembly was 2.33 Gb with a scaffold N50 of 36.7 Mb, and the genome is of comparable size to that of its close relative the polar bear (2.30 Gb). An analysis using 4104 highly conserved mammalian genes indicated that 96.1% were found to be complete within the assembly. An automated annotation of the genome identified 19,848 protein coding genes. Our study shows that the combination of the two sequencing modalities that we used is sufficient for the construction of highly contiguous reference quality mammalian genomes. The assembled genome sequence and the supporting raw sequence reads are available from the NCBI (National Center for Biotechnology Information) under the bioproject identifier PRJNA493656, and the assembly described in this paper is version QXTK01000000. Text Ursus arctos PubMed Central (PMC) Genes 9 12 598 |
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Article Taylor, Gregory A. Kirk, Heather Coombe, Lauren Jackman, Shaun D. Chu, Justin Tse, Kane Cheng, Dean Chuah, Eric Pandoh, Pawan Carlsen, Rebecca Zhao, Yongjun Mungall, Andrew J. Moore, Richard Birol, Inanc Franke, Maria Marra, Marco A. Dutton, Christopher Jones, Steven J. M. The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis |
topic_facet |
Article |
description |
The grizzly bear (Ursus arctos ssp. horribilis) represents the largest population of brown bears in North America. Its genome was sequenced using a microfluidic partitioning library construction technique, and these data were supplemented with sequencing from a nanopore-based long read platform. The final assembly was 2.33 Gb with a scaffold N50 of 36.7 Mb, and the genome is of comparable size to that of its close relative the polar bear (2.30 Gb). An analysis using 4104 highly conserved mammalian genes indicated that 96.1% were found to be complete within the assembly. An automated annotation of the genome identified 19,848 protein coding genes. Our study shows that the combination of the two sequencing modalities that we used is sufficient for the construction of highly contiguous reference quality mammalian genomes. The assembled genome sequence and the supporting raw sequence reads are available from the NCBI (National Center for Biotechnology Information) under the bioproject identifier PRJNA493656, and the assembly described in this paper is version QXTK01000000. |
format |
Text |
author |
Taylor, Gregory A. Kirk, Heather Coombe, Lauren Jackman, Shaun D. Chu, Justin Tse, Kane Cheng, Dean Chuah, Eric Pandoh, Pawan Carlsen, Rebecca Zhao, Yongjun Mungall, Andrew J. Moore, Richard Birol, Inanc Franke, Maria Marra, Marco A. Dutton, Christopher Jones, Steven J. M. |
author_facet |
Taylor, Gregory A. Kirk, Heather Coombe, Lauren Jackman, Shaun D. Chu, Justin Tse, Kane Cheng, Dean Chuah, Eric Pandoh, Pawan Carlsen, Rebecca Zhao, Yongjun Mungall, Andrew J. Moore, Richard Birol, Inanc Franke, Maria Marra, Marco A. Dutton, Christopher Jones, Steven J. M. |
author_sort |
Taylor, Gregory A. |
title |
The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis |
title_short |
The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis |
title_full |
The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis |
title_fullStr |
The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis |
title_full_unstemmed |
The Genome of the North American Brown Bear or Grizzly: Ursus arctos ssp. horribilis |
title_sort |
genome of the north american brown bear or grizzly: ursus arctos ssp. horribilis |
publisher |
MDPI |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315469/ http://www.ncbi.nlm.nih.gov/pubmed/30513700 https://doi.org/10.3390/genes9120598 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315469/ http://www.ncbi.nlm.nih.gov/pubmed/30513700 http://dx.doi.org/10.3390/genes9120598 |
op_rights |
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3390/genes9120598 |
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Genes |
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9 |
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12 |
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598 |
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