No support for cryptic choice by ovarian fluid in an external fertilizer
Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr (Salvelinus alpinus). We physically separated the OF...
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2018
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303707/ http://www.ncbi.nlm.nih.gov/pubmed/30598774 https://doi.org/10.1002/ece3.4628 |
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ftpubmed:oai:pubmedcentral.nih.gov:6303707 2023-05-15T14:30:10+02:00 No support for cryptic choice by ovarian fluid in an external fertilizer Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar 2018-11-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303707/ http://www.ncbi.nlm.nih.gov/pubmed/30598774 https://doi.org/10.1002/ece3.4628 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303707/ http://www.ncbi.nlm.nih.gov/pubmed/30598774 http://dx.doi.org/10.1002/ece3.4628 © 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Original Research Text 2018 ftpubmed https://doi.org/10.1002/ece3.4628 2019-01-06T01:32:55Z Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr (Salvelinus alpinus). We physically separated the OF from the eggs of reproductively active females and reintroduced either their own OF or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split‐brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual OFs. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of OF treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that OF plays an important role as medium for cryptic female choice in charr. Power analyses revealed that our sample size is large enough to detect medium‐sized changes in relative paternity (medium‐sized effect sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about OF's potential influence on paternity under sperm competition—even in charr. Text Arctic charr Arctic Salvelinus alpinus PubMed Central (PMC) Arctic Ecology and Evolution 8 23 11763 11774 |
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Original Research Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar No support for cryptic choice by ovarian fluid in an external fertilizer |
topic_facet |
Original Research |
description |
Whether the ovarian fluid (OF) represents a selective environment influencing cryptic female choice was tested using an external fertilizer experiencing intense sperm competition and large effects of OF on sperm swimming behavior—the Arctic charr (Salvelinus alpinus). We physically separated the OF from the eggs of reproductively active females and reintroduced either their own OF or fluid from another female to the eggs. The eggs were then fertilized in vitro in a replicated split‐brood design with sperm from two males under synchronized sperm competition trials, while also measuring sperm velocity of the individual males in the individual OFs. We found large effects of males, but no effect of females (i.e., eggs) on paternity, determined from microsatellites. More important, we found no effect of OF treatments on the relative paternity of the two competing males in each pair. This experimental setup does not provide support for the hypothesis that OF plays an important role as medium for cryptic female choice in charr. Power analyses revealed that our sample size is large enough to detect medium‐sized changes in relative paternity (medium‐sized effect sizes), but not large enough to detect small changes in relative paternity. More studies are needed before a conclusion can be drawn about OF's potential influence on paternity under sperm competition—even in charr. |
format |
Text |
author |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar |
author_facet |
Kleppe, Snøfrid April Nordeide, Jarle T. Rudolfsen, Geir Figenschou, Lars Larsen, Berner Reiss, Katrin Folstad, Ivar |
author_sort |
Kleppe, Snøfrid April |
title |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_short |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_full |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_fullStr |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_full_unstemmed |
No support for cryptic choice by ovarian fluid in an external fertilizer |
title_sort |
no support for cryptic choice by ovarian fluid in an external fertilizer |
publisher |
John Wiley and Sons Inc. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303707/ http://www.ncbi.nlm.nih.gov/pubmed/30598774 https://doi.org/10.1002/ece3.4628 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic charr Arctic Salvelinus alpinus |
genre_facet |
Arctic charr Arctic Salvelinus alpinus |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303707/ http://www.ncbi.nlm.nih.gov/pubmed/30598774 http://dx.doi.org/10.1002/ece3.4628 |
op_rights |
© 2018 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.4628 |
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Ecology and Evolution |
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8 |
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23 |
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11763 |
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11774 |
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