Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas
Hedgehog signaling pathway participates in a chain of necessary physiological activities and dysregulation of the hedgehog signaling has been implicated in birth defects and diseases. Although substantial studies have uncovered that the hedgehog pathway is both sufficient and necessary for patternin...
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ftpubmed:oai:pubmedcentral.nih.gov:6290081 2023-05-15T15:58:27+02:00 Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas Li, Huijuan Li, Qi Yu, Hong 2018-12-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290081/ https://doi.org/10.3389/fphys.2018.01536 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290081/ http://dx.doi.org/10.3389/fphys.2018.01536 Copyright © 2018 Li, Li and Yu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Physiology Text 2018 ftpubmed https://doi.org/10.3389/fphys.2018.01536 2018-12-23T01:23:00Z Hedgehog signaling pathway participates in a chain of necessary physiological activities and dysregulation of the hedgehog signaling has been implicated in birth defects and diseases. Although substantial studies have uncovered that the hedgehog pathway is both sufficient and necessary for patterning vertebrate muscle differentiation, limited knowledge is available about its role in molluscan myogenesis. Here, the present study firstly identified and characterized the key genes (CgHh, CgPtc, CgSmo, CgGli) in the hedgehog pathway of the Pacific oyster Crassostrea gigas, and investigated the function of this pathway in embryonic myogenesis of C. gigas. Bioinformatics analysis revealed that the functional domains of the key genes were highly conserved among species. Quantitative analysis indicated that CgHh, CgPtc, CgGli mRNA began to accumulate during the blastula to gastrulation stages and accumulated throughout trochophore and into the D-shaped stage. RNA localization patterns by whole-mount in situ hybridization revealed that the key genes own the strongest specific staining in gastrulation, trochophore, and D-shaped stage. Hedgehog pathway genes showed a high expression level in myogenesis stage including trochophore and D-shaped stages, suggesting that the hedgehog pathway would be involved in myogenesis of C. gigas. In adult oysters, the key genes were expressed at various tissues, indicating that hedgehog pathway governed a series of development events. To further examine the role of hedgehog signaling in C. gigas myogenesis, we used cyclopamine treatment in C. gigas larvae to inhibit the signaling pathway. The quantification of the expression of the key genes in hedgehog pathway showed that expressions of key genes were severely down-regulated in treated larvae compared with normal larvae. The velum retractors, ventral retractors, anterior adductor, and posterior adductor muscles of larvae treated with cyclopamine at 4–6 μM for 6–12 h were severely destroyed, suggesting that the hedgehog pathway took part ... Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Frontiers in Physiology 9 |
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Physiology Li, Huijuan Li, Qi Yu, Hong Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas |
topic_facet |
Physiology |
description |
Hedgehog signaling pathway participates in a chain of necessary physiological activities and dysregulation of the hedgehog signaling has been implicated in birth defects and diseases. Although substantial studies have uncovered that the hedgehog pathway is both sufficient and necessary for patterning vertebrate muscle differentiation, limited knowledge is available about its role in molluscan myogenesis. Here, the present study firstly identified and characterized the key genes (CgHh, CgPtc, CgSmo, CgGli) in the hedgehog pathway of the Pacific oyster Crassostrea gigas, and investigated the function of this pathway in embryonic myogenesis of C. gigas. Bioinformatics analysis revealed that the functional domains of the key genes were highly conserved among species. Quantitative analysis indicated that CgHh, CgPtc, CgGli mRNA began to accumulate during the blastula to gastrulation stages and accumulated throughout trochophore and into the D-shaped stage. RNA localization patterns by whole-mount in situ hybridization revealed that the key genes own the strongest specific staining in gastrulation, trochophore, and D-shaped stage. Hedgehog pathway genes showed a high expression level in myogenesis stage including trochophore and D-shaped stages, suggesting that the hedgehog pathway would be involved in myogenesis of C. gigas. In adult oysters, the key genes were expressed at various tissues, indicating that hedgehog pathway governed a series of development events. To further examine the role of hedgehog signaling in C. gigas myogenesis, we used cyclopamine treatment in C. gigas larvae to inhibit the signaling pathway. The quantification of the expression of the key genes in hedgehog pathway showed that expressions of key genes were severely down-regulated in treated larvae compared with normal larvae. The velum retractors, ventral retractors, anterior adductor, and posterior adductor muscles of larvae treated with cyclopamine at 4–6 μM for 6–12 h were severely destroyed, suggesting that the hedgehog pathway took part ... |
format |
Text |
author |
Li, Huijuan Li, Qi Yu, Hong |
author_facet |
Li, Huijuan Li, Qi Yu, Hong |
author_sort |
Li, Huijuan |
title |
Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas |
title_short |
Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas |
title_full |
Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas |
title_fullStr |
Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Molecular Characterization of the Hedgehog Signaling Pathway and Its Necessary Function on Larval Myogenesis in the Pacific Oyster Crassostrea gigas |
title_sort |
molecular characterization of the hedgehog signaling pathway and its necessary function on larval myogenesis in the pacific oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290081/ https://doi.org/10.3389/fphys.2018.01536 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290081/ http://dx.doi.org/10.3389/fphys.2018.01536 |
op_rights |
Copyright © 2018 Li, Li and Yu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2018.01536 |
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Frontiers in Physiology |
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9 |
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1766394195987136512 |