Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats
Microbial mats are ubiquitous in polar freshwater ecosystems and sustain high concentrations of biomass despite the extreme seasonal variations in light and temperature. Here we aimed to resolve genomic adaptations for light-harvesting, bright-light protection, and carbon flow in mats that undergo s...
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ftpubmed:oai:pubmedcentral.nih.gov:6288179 2023-05-15T15:08:39+02:00 Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats Vigneron, Adrien Cruaud, Perrine Mohit, Vani Martineau, Marie-Josée Culley, Alexander I. Lovejoy, Connie Vincent, Warwick F. 2018-12-04 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288179/ http://www.ncbi.nlm.nih.gov/pubmed/30564204 https://doi.org/10.3389/fmicb.2018.02881 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288179/ http://www.ncbi.nlm.nih.gov/pubmed/30564204 http://dx.doi.org/10.3389/fmicb.2018.02881 Copyright © 2018 Vigneron, Cruaud, Mohit, Martineau, Culley, Lovejoy and Vincent. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2018 ftpubmed https://doi.org/10.3389/fmicb.2018.02881 2018-12-23T01:21:21Z Microbial mats are ubiquitous in polar freshwater ecosystems and sustain high concentrations of biomass despite the extreme seasonal variations in light and temperature. Here we aimed to resolve genomic adaptations for light-harvesting, bright-light protection, and carbon flow in mats that undergo seasonal freeze-up. To bracket a range of communities in shallow water habitats, we sampled cyanobacterial mats in the thawed littoral zone of two lakes situated at the northern and southern limits of the Canadian Arctic permafrost zone. We applied a multiphasic approach using pigment profiles from high performance liquid chromatography, Illumina MiSeq sequencing of the 16S and 18S rRNA genes, and metagenomic analysis. The mats shared a taxonomic and functional core microbiome, dominated by oxygenic cyanobacteria with light-harvesting and photoprotective pigments, bacteria with bacteriochlorophyll, and bacteria with light-driven Type I rhodopsins. Organisms able to use light for energy related processes represented up to 85% of the total microbial community, with 15–30% attributable to cyanobacteria and 55–70% attributable to other bacteria. The proportion of genes involved in anaplerotic CO2 fixation was greater than for genes associated with oxygenic photosynthesis. Diverse heterotrophic bacteria, eukaryotes (including metazoans and fungi) and viruses co-occurred in both communities. The results indicate a broad range of strategies for capturing sunlight and CO2, and for the subsequent flow of energy and carbon in these complex, light-driven microbial ecosystems. Text Arctic permafrost PubMed Central (PMC) Arctic Frontiers in Microbiology 9 |
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Microbiology |
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Microbiology Vigneron, Adrien Cruaud, Perrine Mohit, Vani Martineau, Marie-Josée Culley, Alexander I. Lovejoy, Connie Vincent, Warwick F. Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats |
topic_facet |
Microbiology |
description |
Microbial mats are ubiquitous in polar freshwater ecosystems and sustain high concentrations of biomass despite the extreme seasonal variations in light and temperature. Here we aimed to resolve genomic adaptations for light-harvesting, bright-light protection, and carbon flow in mats that undergo seasonal freeze-up. To bracket a range of communities in shallow water habitats, we sampled cyanobacterial mats in the thawed littoral zone of two lakes situated at the northern and southern limits of the Canadian Arctic permafrost zone. We applied a multiphasic approach using pigment profiles from high performance liquid chromatography, Illumina MiSeq sequencing of the 16S and 18S rRNA genes, and metagenomic analysis. The mats shared a taxonomic and functional core microbiome, dominated by oxygenic cyanobacteria with light-harvesting and photoprotective pigments, bacteria with bacteriochlorophyll, and bacteria with light-driven Type I rhodopsins. Organisms able to use light for energy related processes represented up to 85% of the total microbial community, with 15–30% attributable to cyanobacteria and 55–70% attributable to other bacteria. The proportion of genes involved in anaplerotic CO2 fixation was greater than for genes associated with oxygenic photosynthesis. Diverse heterotrophic bacteria, eukaryotes (including metazoans and fungi) and viruses co-occurred in both communities. The results indicate a broad range of strategies for capturing sunlight and CO2, and for the subsequent flow of energy and carbon in these complex, light-driven microbial ecosystems. |
format |
Text |
author |
Vigneron, Adrien Cruaud, Perrine Mohit, Vani Martineau, Marie-Josée Culley, Alexander I. Lovejoy, Connie Vincent, Warwick F. |
author_facet |
Vigneron, Adrien Cruaud, Perrine Mohit, Vani Martineau, Marie-Josée Culley, Alexander I. Lovejoy, Connie Vincent, Warwick F. |
author_sort |
Vigneron, Adrien |
title |
Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats |
title_short |
Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats |
title_full |
Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats |
title_fullStr |
Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats |
title_full_unstemmed |
Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats |
title_sort |
multiple strategies for light-harvesting, photoprotection, and carbon flow in high latitude microbial mats |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288179/ http://www.ncbi.nlm.nih.gov/pubmed/30564204 https://doi.org/10.3389/fmicb.2018.02881 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic permafrost |
genre_facet |
Arctic permafrost |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288179/ http://www.ncbi.nlm.nih.gov/pubmed/30564204 http://dx.doi.org/10.3389/fmicb.2018.02881 |
op_rights |
Copyright © 2018 Vigneron, Cruaud, Mohit, Martineau, Culley, Lovejoy and Vincent. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2018.02881 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
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