Detection of serum antibodies to Brucella in Russian aquatic mammals
A serologic survey of Brucella infection was performed in Caspian seals (Pusa caspica, n=71), Baikal seals (P. sibirica, n=7), ringed seals (P. hispida hispida, n=6), and beluga whales (Delphinapterus leucas, n=4) inhabiting Russian waters, by enzyme-linked immunosorbent assay (ELISA) using Brucella...
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ftpubmed:oai:pubmedcentral.nih.gov:6261826 2023-05-15T15:41:41+02:00 Detection of serum antibodies to Brucella in Russian aquatic mammals OHISHI, Kazue ABE, Erika AMANO, Masao MIYAZAKI, Nobuyuki BOLTUNOV, Andrei KATSUMATA, Etsuko MARUYAMA, Tadashi 2018-09-05 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261826/ http://www.ncbi.nlm.nih.gov/pubmed/30185724 https://doi.org/10.1292/jvms.18-0330 en eng The Japanese Society of Veterinary Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261826/ http://www.ncbi.nlm.nih.gov/pubmed/30185724 http://dx.doi.org/10.1292/jvms.18-0330 ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) CC-BY-NC-ND Wildlife Science Text 2018 ftpubmed https://doi.org/10.1292/jvms.18-0330 2018-12-09T01:21:11Z A serologic survey of Brucella infection was performed in Caspian seals (Pusa caspica, n=71), Baikal seals (P. sibirica, n=7), ringed seals (P. hispida hispida, n=6), and beluga whales (Delphinapterus leucas, n=4) inhabiting Russian waters, by enzyme-linked immunosorbent assay (ELISA) using Brucella abortus and B. canis as antigens. The sera of 4 Caspian seals (4%) tested positive for B. abortus. The same sera samples demonstrated weaker yet detectable affinity for B. canis antigens. Several discrete bands against B. abortus and B. canis antigens were detected on Western blot analysis of the ELISA-positive seal sera; the bands against B. canis were weaker than those against B. abortus. The sera of 3 beluga whales (75%) were positive for B. abortus antigens but showed no binding to B. canis antigens in the ELISA. The positive whale sera showed a strong band appearance only against B. abortus antigens in the Western blot analysis. Many detected bands were discrete, while some of them had a smeared appearance. The present results indicate that Brucella infection occurred in Caspian seals and beluga whales inhabiting Russian waters, and that the Brucella strains infecting the seals and the whales were antigenetically distinct. Text Beluga Beluga* Delphinapterus leucas PubMed Central (PMC) Journal of Veterinary Medical Science 80 11 1696 1701 |
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Wildlife Science OHISHI, Kazue ABE, Erika AMANO, Masao MIYAZAKI, Nobuyuki BOLTUNOV, Andrei KATSUMATA, Etsuko MARUYAMA, Tadashi Detection of serum antibodies to Brucella in Russian aquatic mammals |
topic_facet |
Wildlife Science |
description |
A serologic survey of Brucella infection was performed in Caspian seals (Pusa caspica, n=71), Baikal seals (P. sibirica, n=7), ringed seals (P. hispida hispida, n=6), and beluga whales (Delphinapterus leucas, n=4) inhabiting Russian waters, by enzyme-linked immunosorbent assay (ELISA) using Brucella abortus and B. canis as antigens. The sera of 4 Caspian seals (4%) tested positive for B. abortus. The same sera samples demonstrated weaker yet detectable affinity for B. canis antigens. Several discrete bands against B. abortus and B. canis antigens were detected on Western blot analysis of the ELISA-positive seal sera; the bands against B. canis were weaker than those against B. abortus. The sera of 3 beluga whales (75%) were positive for B. abortus antigens but showed no binding to B. canis antigens in the ELISA. The positive whale sera showed a strong band appearance only against B. abortus antigens in the Western blot analysis. Many detected bands were discrete, while some of them had a smeared appearance. The present results indicate that Brucella infection occurred in Caspian seals and beluga whales inhabiting Russian waters, and that the Brucella strains infecting the seals and the whales were antigenetically distinct. |
format |
Text |
author |
OHISHI, Kazue ABE, Erika AMANO, Masao MIYAZAKI, Nobuyuki BOLTUNOV, Andrei KATSUMATA, Etsuko MARUYAMA, Tadashi |
author_facet |
OHISHI, Kazue ABE, Erika AMANO, Masao MIYAZAKI, Nobuyuki BOLTUNOV, Andrei KATSUMATA, Etsuko MARUYAMA, Tadashi |
author_sort |
OHISHI, Kazue |
title |
Detection of serum antibodies to Brucella in Russian aquatic mammals |
title_short |
Detection of serum antibodies to Brucella in Russian aquatic mammals |
title_full |
Detection of serum antibodies to Brucella in Russian aquatic mammals |
title_fullStr |
Detection of serum antibodies to Brucella in Russian aquatic mammals |
title_full_unstemmed |
Detection of serum antibodies to Brucella in Russian aquatic mammals |
title_sort |
detection of serum antibodies to brucella in russian aquatic mammals |
publisher |
The Japanese Society of Veterinary Science |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261826/ http://www.ncbi.nlm.nih.gov/pubmed/30185724 https://doi.org/10.1292/jvms.18-0330 |
genre |
Beluga Beluga* Delphinapterus leucas |
genre_facet |
Beluga Beluga* Delphinapterus leucas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261826/ http://www.ncbi.nlm.nih.gov/pubmed/30185724 http://dx.doi.org/10.1292/jvms.18-0330 |
op_rights |
©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1292/jvms.18-0330 |
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Journal of Veterinary Medical Science |
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80 |
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11 |
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1696 |
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1701 |
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1766374570791534592 |