An improved environmental DNA assay for bull trout (Salvelinus confluentus) based on the ribosomal internal transcribed spacer I

• Published in: PLOS ONE
• Main Authors: Dysthe, Joseph C., Franklin, Thomas W., McKelvey, Kevin S., Young, Michael K., Schwartz, Michael K.
• Format: Text
• Language: English
• Published: Public Library of Science 2018
• Subjects: Research Article; Arctic; Varden
• Online Access: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219789/; http://www.ncbi.nlm.nih.gov/pubmed/30399172; https://doi.org/10.1371/journal.pone.0206851

The majority of environmental DNA (eDNA) assays for vertebrate species are based on commonly analyzed regions of the mitochondrial genome. However, the high degree of mitochondrial similarity between two species of charr (Salvelinus spp.), southern Dolly Varden (S. malma lordii) and bull trout (Salv...