Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs
The Papaver spp. (Papaver rhoeas (Corn poppy) and Papaver nudicaule (Iceland poppy)) genera are ornamental and medicinal plants that are used for the isolation of alkaloid drugs. In this study, we generated 700 Mb of transcriptome sequences with the PacBio platform. They were assembled into 120,926...
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ftpubmed:oai:pubmedcentral.nih.gov:6213990 2023-05-15T16:50:34+02:00 Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs Oh, Jaehyeon Shin, Younhee Ha, In Jin Lee, Min Young Lee, Seok-Geun Kang, Byeong-Chul Kyeong, Dongsoo Kim, Dowan 2018-10-16 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213990/ http://www.ncbi.nlm.nih.gov/pubmed/30332811 https://doi.org/10.3390/ijms19103192 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213990/ http://www.ncbi.nlm.nih.gov/pubmed/30332811 http://dx.doi.org/10.3390/ijms19103192 © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2018 ftpubmed https://doi.org/10.3390/ijms19103192 2018-11-18T02:05:25Z The Papaver spp. (Papaver rhoeas (Corn poppy) and Papaver nudicaule (Iceland poppy)) genera are ornamental and medicinal plants that are used for the isolation of alkaloid drugs. In this study, we generated 700 Mb of transcriptome sequences with the PacBio platform. They were assembled into 120,926 contigs, and 1185 (82.2%) of the benchmarking universal single-copy orthologs (BUSCO) core genes were completely present in our assembled transcriptome. Furthermore, using 128 Gb of Illumina sequences, the transcript expression was assessed at three stages of Papaver plant development (30, 60, and 90 days), from which we identified 137 differentially expressed transcripts. Furthermore, three co-occurrence heat maps are generated from 51 different plant genomes along with the Papaver transcriptome, i.e., secondary metabolite biosynthesis, isoquinoline alkaloid biosynthesis (BIA) pathway, and cytochrome. Sixty-nine transcripts in the BIA pathway along with 22 different alkaloids (quantified with LC-QTOF-MS/MS) were mapped into the BIA KEGG map (map00950). Finally, we identified 39 full-length cytochrome transcripts and compared them with other genomes. Collectively, this transcriptome data, along with the expression and quantitative metabolite profiles, provides an initial recording of secondary metabolites and their expression related to Papaver plant development. Moreover, these profiles could help to further detail the functional characterization of the various secondary metabolite biosynthesis and Papaver plant development associated problems. Text Iceland PubMed Central (PMC) Bia ENVELOPE(22.891,22.891,70.317,70.317) International Journal of Molecular Sciences 19 10 3192 |
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Article Oh, Jaehyeon Shin, Younhee Ha, In Jin Lee, Min Young Lee, Seok-Geun Kang, Byeong-Chul Kyeong, Dongsoo Kim, Dowan Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs |
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The Papaver spp. (Papaver rhoeas (Corn poppy) and Papaver nudicaule (Iceland poppy)) genera are ornamental and medicinal plants that are used for the isolation of alkaloid drugs. In this study, we generated 700 Mb of transcriptome sequences with the PacBio platform. They were assembled into 120,926 contigs, and 1185 (82.2%) of the benchmarking universal single-copy orthologs (BUSCO) core genes were completely present in our assembled transcriptome. Furthermore, using 128 Gb of Illumina sequences, the transcript expression was assessed at three stages of Papaver plant development (30, 60, and 90 days), from which we identified 137 differentially expressed transcripts. Furthermore, three co-occurrence heat maps are generated from 51 different plant genomes along with the Papaver transcriptome, i.e., secondary metabolite biosynthesis, isoquinoline alkaloid biosynthesis (BIA) pathway, and cytochrome. Sixty-nine transcripts in the BIA pathway along with 22 different alkaloids (quantified with LC-QTOF-MS/MS) were mapped into the BIA KEGG map (map00950). Finally, we identified 39 full-length cytochrome transcripts and compared them with other genomes. Collectively, this transcriptome data, along with the expression and quantitative metabolite profiles, provides an initial recording of secondary metabolites and their expression related to Papaver plant development. Moreover, these profiles could help to further detail the functional characterization of the various secondary metabolite biosynthesis and Papaver plant development associated problems. |
format |
Text |
author |
Oh, Jaehyeon Shin, Younhee Ha, In Jin Lee, Min Young Lee, Seok-Geun Kang, Byeong-Chul Kyeong, Dongsoo Kim, Dowan |
author_facet |
Oh, Jaehyeon Shin, Younhee Ha, In Jin Lee, Min Young Lee, Seok-Geun Kang, Byeong-Chul Kyeong, Dongsoo Kim, Dowan |
author_sort |
Oh, Jaehyeon |
title |
Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs |
title_short |
Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs |
title_full |
Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs |
title_fullStr |
Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs |
title_full_unstemmed |
Transcriptome Profiling of Two Ornamental and Medicinal Papaver Herbs |
title_sort |
transcriptome profiling of two ornamental and medicinal papaver herbs |
publisher |
MDPI |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213990/ http://www.ncbi.nlm.nih.gov/pubmed/30332811 https://doi.org/10.3390/ijms19103192 |
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ENVELOPE(22.891,22.891,70.317,70.317) |
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Bia |
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Bia |
genre |
Iceland |
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Iceland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6213990/ http://www.ncbi.nlm.nih.gov/pubmed/30332811 http://dx.doi.org/10.3390/ijms19103192 |
op_rights |
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms19103192 |
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International Journal of Molecular Sciences |
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19 |
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10 |
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3192 |
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1766040708351787008 |