Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation
Spermatogenesis is a dynamic developmental process in which spermatogonial stem cells proliferate, differentiate and mature into functional spermatozoa. These processes require an accurate gene regulation network. Here, we investigated the dynamic changes that occur during spermatogenesis through a...
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ftpubmed:oai:pubmedcentral.nih.gov:6202422 2023-05-15T18:15:49+02:00 Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation Wang, Xueying Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Yanfeng Feng, Chengcheng Xue, Rui Zhao, Haixia Song, Zongcheng Li, Jun 2018-10-25 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202422/ http://www.ncbi.nlm.nih.gov/pubmed/30361543 https://doi.org/10.1038/s41598-018-34149-5 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202422/ http://www.ncbi.nlm.nih.gov/pubmed/30361543 http://dx.doi.org/10.1038/s41598-018-34149-5 © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2018 ftpubmed https://doi.org/10.1038/s41598-018-34149-5 2018-11-04T01:45:29Z Spermatogenesis is a dynamic developmental process in which spermatogonial stem cells proliferate, differentiate and mature into functional spermatozoa. These processes require an accurate gene regulation network. Here, we investigated the dynamic changes that occur during spermatogenesis through a combination of histological and transcriptome analyses of different developmental stages of the testis. We constructed 18 testis transcriptome libraries, and the average length, N50, and GC content of the unigenes were 1,795 bp; 3,240 bp and 49.25%, respectively. Differentially expressed genes (DEGs) that were related to germ cell proliferation and maturation, such as NANOS3, RARs, KIFs, steroid hormone synthesis-related genes and receptor genes, were identified between pairs of testis at different developmental stages. Gene ontology annotation and pathway analyses were conducted on DEGs with specific expression patterns involved in the regulation of spermatogenesis. Nine important pathways such as steroid hormone biosynthesis related to spermatogenesis were identified. A total of 21 modules that ranged from 49 to 7,448 genes were designed by a weighted gene co-expression network analysis. Furthermore, a total of 83 candidate miRNA were identified by computational methods. Our study provides the first transcriptomic evidence for differences in gene expression between different developmental stages of spermatogenesis in turbot (Scophthalmus maximus). Text Scophthalmus maximus Turbot PubMed Central (PMC) Scientific Reports 8 1 |
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Article Wang, Xueying Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Yanfeng Feng, Chengcheng Xue, Rui Zhao, Haixia Song, Zongcheng Li, Jun Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation |
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Article |
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Spermatogenesis is a dynamic developmental process in which spermatogonial stem cells proliferate, differentiate and mature into functional spermatozoa. These processes require an accurate gene regulation network. Here, we investigated the dynamic changes that occur during spermatogenesis through a combination of histological and transcriptome analyses of different developmental stages of the testis. We constructed 18 testis transcriptome libraries, and the average length, N50, and GC content of the unigenes were 1,795 bp; 3,240 bp and 49.25%, respectively. Differentially expressed genes (DEGs) that were related to germ cell proliferation and maturation, such as NANOS3, RARs, KIFs, steroid hormone synthesis-related genes and receptor genes, were identified between pairs of testis at different developmental stages. Gene ontology annotation and pathway analyses were conducted on DEGs with specific expression patterns involved in the regulation of spermatogenesis. Nine important pathways such as steroid hormone biosynthesis related to spermatogenesis were identified. A total of 21 modules that ranged from 49 to 7,448 genes were designed by a weighted gene co-expression network analysis. Furthermore, a total of 83 candidate miRNA were identified by computational methods. Our study provides the first transcriptomic evidence for differences in gene expression between different developmental stages of spermatogenesis in turbot (Scophthalmus maximus). |
format |
Text |
author |
Wang, Xueying Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Yanfeng Feng, Chengcheng Xue, Rui Zhao, Haixia Song, Zongcheng Li, Jun |
author_facet |
Wang, Xueying Liu, Qinghua Xu, Shihong Xiao, Yongshuang Wang, Yanfeng Feng, Chengcheng Xue, Rui Zhao, Haixia Song, Zongcheng Li, Jun |
author_sort |
Wang, Xueying |
title |
Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation |
title_short |
Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation |
title_full |
Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation |
title_fullStr |
Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation |
title_full_unstemmed |
Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation |
title_sort |
transcriptome dynamics during turbot spermatogenesis predicting the potential key genes regulating male germ cell proliferation and maturation |
publisher |
Nature Publishing Group UK |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202422/ http://www.ncbi.nlm.nih.gov/pubmed/30361543 https://doi.org/10.1038/s41598-018-34149-5 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202422/ http://www.ncbi.nlm.nih.gov/pubmed/30361543 http://dx.doi.org/10.1038/s41598-018-34149-5 |
op_rights |
© The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-018-34149-5 |
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Scientific Reports |
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