Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagi...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Nature Publishing Group UK
2018
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193008/ http://www.ncbi.nlm.nih.gov/pubmed/30333557 https://doi.org/10.1038/s41598-018-33720-4 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:6193008 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:6193008 2023-05-15T15:57:58+02:00 Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre 2018-10-17 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193008/ http://www.ncbi.nlm.nih.gov/pubmed/30333557 https://doi.org/10.1038/s41598-018-33720-4 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193008/ http://www.ncbi.nlm.nih.gov/pubmed/30333557 http://dx.doi.org/10.1038/s41598-018-33720-4 © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2018 ftpubmed https://doi.org/10.1038/s41598-018-33720-4 2018-10-28T00:21:29Z Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement. Text Crassostrea gigas PubMed Central (PMC) Scientific Reports 8 1 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Article |
spellingShingle |
Article Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
topic_facet |
Article |
description |
Bioadhesion of marine organisms has been intensively studied over the last decade because of their ability to attach in various wet environmental conditions and the potential this offers for biotechnology applications. Many marine mollusc species are characterized by a two-phase life history: pelagic larvae settle prior to metamorphosis to a benthic stage. The oyster Crassostrea gigas has been extensively studied for its economic and ecological importance. However, the bioadhesive produced by ready to settle larvae of this species has been little studied. The pediveliger stage of oysters is characterized by the genesis of a specific organ essential for adhesion, the foot. Our scanning electron microscopy and histology analysis revealed that in C. gigas the adhesive is produced by several foot glands. This adhesive is composed of numerous fibres of differing structure, suggesting differences in chemical composition and function. Fourier transformed infrared spectroscopy indicated a mainly proteinaceous composition. Proteomic analysis of footprints was able to identify 42 proteins, among which, one uncharacterized protein was selected on the basis of its pediveliger transcriptome specificity and then located by mRNA in situ hybridization, revealing its potential role during substrate exploration before oyster larva settlement. |
format |
Text |
author |
Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre |
author_facet |
Foulon, Valentin Artigaud, Sébastien Buscaglia, Manon Bernay, Benoit Fabioux, Caroline Petton, Bruno Elies, Philippe Boukerma, Kada Hellio, Claire Guérard, Fabienne Boudry, Pierre |
author_sort |
Foulon, Valentin |
title |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_short |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_full |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_fullStr |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_full_unstemmed |
Proteinaceous secretion of bioadhesive produced during crawling and settlement of Crassostrea gigas larvae |
title_sort |
proteinaceous secretion of bioadhesive produced during crawling and settlement of crassostrea gigas larvae |
publisher |
Nature Publishing Group UK |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193008/ http://www.ncbi.nlm.nih.gov/pubmed/30333557 https://doi.org/10.1038/s41598-018-33720-4 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6193008/ http://www.ncbi.nlm.nih.gov/pubmed/30333557 http://dx.doi.org/10.1038/s41598-018-33720-4 |
op_rights |
© The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-018-33720-4 |
container_title |
Scientific Reports |
container_volume |
8 |
container_issue |
1 |
_version_ |
1766393687455039488 |