Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment
Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies‐carbon‐flow during anaerobic OM degradatio...
Published in: | Environmental Microbiology |
---|---|
Main Authors: | , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
John Wiley and Sons Inc.
2018
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175234/ http://www.ncbi.nlm.nih.gov/pubmed/30051650 https://doi.org/10.1111/1462-2920.14297 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:6175234 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:6175234 2023-05-15T14:48:12+02:00 Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment Müller, Albert L. Pelikan, Claus de Rezende, Julia R. Wasmund, Kenneth Putz, Martina Glombitza, Clemens Kjeldsen, Kasper U. Jørgensen, Bo Barker Loy, Alexander 2018-09-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175234/ http://www.ncbi.nlm.nih.gov/pubmed/30051650 https://doi.org/10.1111/1462-2920.14297 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175234/ http://www.ncbi.nlm.nih.gov/pubmed/30051650 http://dx.doi.org/10.1111/1462-2920.14297 © 2018 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Research Articles Text 2018 ftpubmed https://doi.org/10.1111/1462-2920.14297 2018-10-21T00:17:07Z Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies‐carbon‐flow during anaerobic OM degradation in arctic marine sediment using stable isotope probing. We supplemented sediment incubations with 13C‐labeled cyanobacterial necromass (spirulina), mimicking fresh OM input, or acetate, an important OM degradation intermediate and monitored sulfate reduction rates and concentrations of volatile fatty acids (VFAs) during substrate degradation. Sequential 16S rRNA gene and transcript amplicon sequencing and fluorescence in situ hybridization combined with Raman microspectroscopy revealed that only few bacterial species were the main degraders of 13C‐spirulina necromass. Psychrilyobacter, Psychromonas, Marinifilum, Colwellia, Marinilabiaceae and Clostridiales species were likely involved in the primary hydrolysis and fermentation of spirulina. VFAs, mainly acetate, produced from spirulina degradation were mineralized by sulfate‐reducing bacteria and an Arcobacter species. Cellular activity of Desulfobacteraceae and Desulfobulbaceae species during acetoclastic sulfate reduction was largely decoupled from relative 16S rRNA gene abundance shifts. Our findings provide new insights into the identities and physiological constraints that determine the population dynamics of key microorganisms during complex OM degradation in arctic marine sediments.© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd Text Arctic Sea ice PubMed Central (PMC) Arctic Environmental Microbiology 20 8 2927 2940 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Articles |
spellingShingle |
Research Articles Müller, Albert L. Pelikan, Claus de Rezende, Julia R. Wasmund, Kenneth Putz, Martina Glombitza, Clemens Kjeldsen, Kasper U. Jørgensen, Bo Barker Loy, Alexander Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment |
topic_facet |
Research Articles |
description |
Seafloor microorganisms impact global carbon cycling by mineralizing vast quantities of organic matter (OM) from pelagic primary production, which is predicted to increase in the Arctic because of diminishing sea ice cover. We studied microbial interspecies‐carbon‐flow during anaerobic OM degradation in arctic marine sediment using stable isotope probing. We supplemented sediment incubations with 13C‐labeled cyanobacterial necromass (spirulina), mimicking fresh OM input, or acetate, an important OM degradation intermediate and monitored sulfate reduction rates and concentrations of volatile fatty acids (VFAs) during substrate degradation. Sequential 16S rRNA gene and transcript amplicon sequencing and fluorescence in situ hybridization combined with Raman microspectroscopy revealed that only few bacterial species were the main degraders of 13C‐spirulina necromass. Psychrilyobacter, Psychromonas, Marinifilum, Colwellia, Marinilabiaceae and Clostridiales species were likely involved in the primary hydrolysis and fermentation of spirulina. VFAs, mainly acetate, produced from spirulina degradation were mineralized by sulfate‐reducing bacteria and an Arcobacter species. Cellular activity of Desulfobacteraceae and Desulfobulbaceae species during acetoclastic sulfate reduction was largely decoupled from relative 16S rRNA gene abundance shifts. Our findings provide new insights into the identities and physiological constraints that determine the population dynamics of key microorganisms during complex OM degradation in arctic marine sediments.© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd |
format |
Text |
author |
Müller, Albert L. Pelikan, Claus de Rezende, Julia R. Wasmund, Kenneth Putz, Martina Glombitza, Clemens Kjeldsen, Kasper U. Jørgensen, Bo Barker Loy, Alexander |
author_facet |
Müller, Albert L. Pelikan, Claus de Rezende, Julia R. Wasmund, Kenneth Putz, Martina Glombitza, Clemens Kjeldsen, Kasper U. Jørgensen, Bo Barker Loy, Alexander |
author_sort |
Müller, Albert L. |
title |
Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment |
title_short |
Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment |
title_full |
Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment |
title_fullStr |
Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment |
title_full_unstemmed |
Bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment |
title_sort |
bacterial interactions during sequential degradation of cyanobacterial necromass in a sulfidic arctic marine sediment |
publisher |
John Wiley and Sons Inc. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175234/ http://www.ncbi.nlm.nih.gov/pubmed/30051650 https://doi.org/10.1111/1462-2920.14297 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Sea ice |
genre_facet |
Arctic Sea ice |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175234/ http://www.ncbi.nlm.nih.gov/pubmed/30051650 http://dx.doi.org/10.1111/1462-2920.14297 |
op_rights |
© 2018 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/1462-2920.14297 |
container_title |
Environmental Microbiology |
container_volume |
20 |
container_issue |
8 |
container_start_page |
2927 |
op_container_end_page |
2940 |
_version_ |
1766319299050340352 |