Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida

Edwardsiella piscicida is a leading fish pathogen that causes significant economic loses in the aquaculture industry. The pathogen depends on type III and type VI secretion systems (T3/T6SS) for growth and virulence in fish and the expression of both systems is controlled by the EsrB transcription a...

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Published in:Aquaculture Reports
Main Authors: Yin, Kaiyu, Guan, Yunpeng, Ma, Ruiqing, Wei, Lifan, Liu, Bing, Liu, Xiaohong, Zhou, Xiangshan, Ma, Yue, Zhang, Yuanxing, Waldor, Matthew K., Wang, Qiyao
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Language:English
Published: Public Library of Science 2018
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Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6136808/
http://www.ncbi.nlm.nih.gov/pubmed/30169545
https://doi.org/10.1371/journal.ppat.1007272
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spelling ftpubmed:oai:pubmedcentral.nih.gov:6136808 2023-05-15T18:41:14+02:00 Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida Yin, Kaiyu Guan, Yunpeng Ma, Ruiqing Wei, Lifan Liu, Bing Liu, Xiaohong Zhou, Xiangshan Ma, Yue Zhang, Yuanxing Waldor, Matthew K. Wang, Qiyao 2018-08-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6136808/ http://www.ncbi.nlm.nih.gov/pubmed/30169545 https://doi.org/10.1371/journal.ppat.1007272 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6136808/ http://www.ncbi.nlm.nih.gov/pubmed/30169545 http://dx.doi.org/10.1371/journal.ppat.1007272 © 2018 Yin et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2018 ftpubmed https://doi.org/10.1371/journal.ppat.1007272 2018-09-30T00:13:43Z Edwardsiella piscicida is a leading fish pathogen that causes significant economic loses in the aquaculture industry. The pathogen depends on type III and type VI secretion systems (T3/T6SS) for growth and virulence in fish and the expression of both systems is controlled by the EsrB transcription activator. Here, we performed a Tn-seq-based screen to uncover factors that govern esrB expression. Unexpectedly, we discovered that RpoS antagonizes esrB expression and thereby inhibits production of E. piscicida’s T3/T6SS. Using in vitro transcription assays, we showed that RpoS can block RpoD-mediated transcription of esrB. ChIP-seq- and RNA-seq-based profiling, as well as mutational and biochemical analyses revealed that RpoS-repressed promoters contain a -6G in their respective discriminator sequences; moreover, this -6G proved critical for RpoS to inhibit esrB expression. Mutation of the RpoS R99 residue, an amino acid that molecular modeling predicts interacts with -6G in the esrB discriminator, abolished RpoS’ capacity for repression. In a turbot model, an rpoS deletion mutant was attenuated early but not late in infection, whereas a mutant expressing RpoSR99A exhibited elevated fitness throughout the infection period. Collectively, these findings deepen our understanding of how RpoS can inhibit gene expression and demonstrate the temporal variation in the requirement for this sigma factor during infection. Text Turbot PubMed Central (PMC) Aquaculture Reports 4 36 41
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Research Article
spellingShingle Research Article
Yin, Kaiyu
Guan, Yunpeng
Ma, Ruiqing
Wei, Lifan
Liu, Bing
Liu, Xiaohong
Zhou, Xiangshan
Ma, Yue
Zhang, Yuanxing
Waldor, Matthew K.
Wang, Qiyao
Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida
topic_facet Research Article
description Edwardsiella piscicida is a leading fish pathogen that causes significant economic loses in the aquaculture industry. The pathogen depends on type III and type VI secretion systems (T3/T6SS) for growth and virulence in fish and the expression of both systems is controlled by the EsrB transcription activator. Here, we performed a Tn-seq-based screen to uncover factors that govern esrB expression. Unexpectedly, we discovered that RpoS antagonizes esrB expression and thereby inhibits production of E. piscicida’s T3/T6SS. Using in vitro transcription assays, we showed that RpoS can block RpoD-mediated transcription of esrB. ChIP-seq- and RNA-seq-based profiling, as well as mutational and biochemical analyses revealed that RpoS-repressed promoters contain a -6G in their respective discriminator sequences; moreover, this -6G proved critical for RpoS to inhibit esrB expression. Mutation of the RpoS R99 residue, an amino acid that molecular modeling predicts interacts with -6G in the esrB discriminator, abolished RpoS’ capacity for repression. In a turbot model, an rpoS deletion mutant was attenuated early but not late in infection, whereas a mutant expressing RpoSR99A exhibited elevated fitness throughout the infection period. Collectively, these findings deepen our understanding of how RpoS can inhibit gene expression and demonstrate the temporal variation in the requirement for this sigma factor during infection.
format Text
author Yin, Kaiyu
Guan, Yunpeng
Ma, Ruiqing
Wei, Lifan
Liu, Bing
Liu, Xiaohong
Zhou, Xiangshan
Ma, Yue
Zhang, Yuanxing
Waldor, Matthew K.
Wang, Qiyao
author_facet Yin, Kaiyu
Guan, Yunpeng
Ma, Ruiqing
Wei, Lifan
Liu, Bing
Liu, Xiaohong
Zhou, Xiangshan
Ma, Yue
Zhang, Yuanxing
Waldor, Matthew K.
Wang, Qiyao
author_sort Yin, Kaiyu
title Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida
title_short Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida
title_full Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida
title_fullStr Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida
title_full_unstemmed Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida
title_sort critical role for a promoter discriminator in rpos control of virulence in edwardsiella piscicida
publisher Public Library of Science
publishDate 2018
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6136808/
http://www.ncbi.nlm.nih.gov/pubmed/30169545
https://doi.org/10.1371/journal.ppat.1007272
genre Turbot
genre_facet Turbot
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6136808/
http://www.ncbi.nlm.nih.gov/pubmed/30169545
http://dx.doi.org/10.1371/journal.ppat.1007272
op_rights © 2018 Yin et al
http://creativecommons.org/licenses/by/4.0/
This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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op_doi https://doi.org/10.1371/journal.ppat.1007272
container_title Aquaculture Reports
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