Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise (Phocoena phocoena), o...
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ftpubmed:oai:pubmedcentral.nih.gov:6124077 2023-05-15T16:33:25+02:00 Water, water everywhere: environmental DNA can unlock population structure in elusive marine species Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda 2018-08-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124077/ http://www.ncbi.nlm.nih.gov/pubmed/30225045 https://doi.org/10.1098/rsos.180537 en eng The Royal Society Publishing http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124077/ http://www.ncbi.nlm.nih.gov/pubmed/30225045 http://dx.doi.org/10.1098/rsos.180537 © 2018 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. CC-BY Genetics and Genomics Text 2018 ftpubmed https://doi.org/10.1098/rsos.180537 2018-09-23T00:10:40Z Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise (Phocoena phocoena), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. Text Harbour porpoise Phocoena phocoena Alaska PubMed Central (PMC) Royal Society Open Science 5 8 180537 |
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topic |
Genetics and Genomics |
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Genetics and Genomics Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
topic_facet |
Genetics and Genomics |
description |
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise (Phocoena phocoena), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. |
format |
Text |
author |
Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda |
author_facet |
Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda |
author_sort |
Parsons, Kim M. |
title |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_short |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_fullStr |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full_unstemmed |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_sort |
water, water everywhere: environmental dna can unlock population structure in elusive marine species |
publisher |
The Royal Society Publishing |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124077/ http://www.ncbi.nlm.nih.gov/pubmed/30225045 https://doi.org/10.1098/rsos.180537 |
genre |
Harbour porpoise Phocoena phocoena Alaska |
genre_facet |
Harbour porpoise Phocoena phocoena Alaska |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124077/ http://www.ncbi.nlm.nih.gov/pubmed/30225045 http://dx.doi.org/10.1098/rsos.180537 |
op_rights |
© 2018 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1098/rsos.180537 |
container_title |
Royal Society Open Science |
container_volume |
5 |
container_issue |
8 |
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180537 |
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1766023110433177600 |