Metagenomic insights into diazotrophic communities across Arctic glacier forefields
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In to...
Published in: | FEMS Microbiology Ecology |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Oxford University Press
2018
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054269/ http://www.ncbi.nlm.nih.gov/pubmed/29901729 https://doi.org/10.1093/femsec/fiy114 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:6054269 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:6054269 2023-05-15T14:56:09+02:00 Metagenomic insights into diazotrophic communities across Arctic glacier forefields Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia 2018-06-12 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054269/ http://www.ncbi.nlm.nih.gov/pubmed/29901729 https://doi.org/10.1093/femsec/fiy114 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054269/ http://www.ncbi.nlm.nih.gov/pubmed/29901729 http://dx.doi.org/10.1093/femsec/fiy114 © FEMS 2018. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2018 ftpubmed https://doi.org/10.1093/femsec/fiy114 2018-07-29T00:28:06Z Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. Text Arctic PubMed Central (PMC) Arctic FEMS Microbiology Ecology 94 9 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
topic_facet |
Research Article |
description |
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. |
format |
Text |
author |
Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia |
author_facet |
Nash, Maisie V Anesio, Alexandre M Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G Sánchez-Baracaldo, Patricia |
author_sort |
Nash, Maisie V |
title |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_short |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_fullStr |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full_unstemmed |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_sort |
metagenomic insights into diazotrophic communities across arctic glacier forefields |
publisher |
Oxford University Press |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054269/ http://www.ncbi.nlm.nih.gov/pubmed/29901729 https://doi.org/10.1093/femsec/fiy114 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054269/ http://www.ncbi.nlm.nih.gov/pubmed/29901729 http://dx.doi.org/10.1093/femsec/fiy114 |
op_rights |
© FEMS 2018. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1093/femsec/fiy114 |
container_title |
FEMS Microbiology Ecology |
container_volume |
94 |
container_issue |
9 |
_version_ |
1766328179705774080 |