Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis
Fatty acids are one of the most important nutrients in food. Acid- or base-catalyzed transesterification methods are commonly used for the analysis of fatty acids. However, several drawbacks were reported for these methods, including the isomerization and degradation of fatty acids. Lipase-catalyzed...
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The Korean Society of Food Science and Technology
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ftpubmed:oai:pubmedcentral.nih.gov:6049160 2023-05-15T14:15:31+02:00 Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis Kim, Chang-Soo Lee, Si-Kyung Keum, Young Soo 2016-06-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049160/ https://doi.org/10.1007/s10068-016-0131-6 en eng The Korean Society of Food Science and Technology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049160/ http://dx.doi.org/10.1007/s10068-016-0131-6 © The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2016 Article Text 2016 ftpubmed https://doi.org/10.1007/s10068-016-0131-6 2018-09-30T00:08:30Z Fatty acids are one of the most important nutrients in food. Acid- or base-catalyzed transesterification methods are commonly used for the analysis of fatty acids. However, several drawbacks were reported for these methods, including the isomerization and degradation of fatty acids. Lipase-catalyzed reactions are usually undertaken at mild conditions, preventing such problems. In this study, commercial resin-bound lipase from Candida antartica was tested for possible application in fatty acid methyl ester analysis. Experimental parameters, including temperature, reaction time, and re-cycling were evaluated. The optimized condition was (5-10 mg lipid, 0.5 mL of MeOH, and 50 mg Novozyme 435 in 2 mL toluene, 80°C for 1 h). In optimized condition, the lipase-catalyzed methods yielded similar results with the chemical method. In overall, lipase-catalyzed transesterification can be a useful alternative to acid-catalyzed methods for fatty acid analysis. Text antartic* PubMed Central (PMC) Food Science and Biotechnology 25 3 771 776 |
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Article Kim, Chang-Soo Lee, Si-Kyung Keum, Young Soo Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis |
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Article |
description |
Fatty acids are one of the most important nutrients in food. Acid- or base-catalyzed transesterification methods are commonly used for the analysis of fatty acids. However, several drawbacks were reported for these methods, including the isomerization and degradation of fatty acids. Lipase-catalyzed reactions are usually undertaken at mild conditions, preventing such problems. In this study, commercial resin-bound lipase from Candida antartica was tested for possible application in fatty acid methyl ester analysis. Experimental parameters, including temperature, reaction time, and re-cycling were evaluated. The optimized condition was (5-10 mg lipid, 0.5 mL of MeOH, and 50 mg Novozyme 435 in 2 mL toluene, 80°C for 1 h). In optimized condition, the lipase-catalyzed methods yielded similar results with the chemical method. In overall, lipase-catalyzed transesterification can be a useful alternative to acid-catalyzed methods for fatty acid analysis. |
format |
Text |
author |
Kim, Chang-Soo Lee, Si-Kyung Keum, Young Soo |
author_facet |
Kim, Chang-Soo Lee, Si-Kyung Keum, Young Soo |
author_sort |
Kim, Chang-Soo |
title |
Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis |
title_short |
Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis |
title_full |
Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis |
title_fullStr |
Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis |
title_full_unstemmed |
Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis |
title_sort |
comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis |
publisher |
The Korean Society of Food Science and Technology |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049160/ https://doi.org/10.1007/s10068-016-0131-6 |
genre |
antartic* |
genre_facet |
antartic* |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6049160/ http://dx.doi.org/10.1007/s10068-016-0131-6 |
op_rights |
© The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht 2016 |
op_doi |
https://doi.org/10.1007/s10068-016-0131-6 |
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Food Science and Biotechnology |
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25 |
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3 |
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776 |
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