First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran
The purpose of the present study was to isolate and identify the pathogenic agents in Acipenser stellatus (Pallas, 1771) and Huso huso, (Linnaeus, 1758) reared in the south of Fars province, Iran which have shown infectious disease signs. Samples from spleen and kidney of 32 fishes showing septicemi...
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ftpubmed:oai:pubmedcentral.nih.gov:6047580 2023-05-15T15:41:52+02:00 First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran Gholamhosseini, Amin Taghadosi, Vahideh Shiry, Nima Akhlaghi, Mostafa Sharifiyazdi, Hassan Soltanian, Siyavash Ahmadi, Nasrollah 2018 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047580/ http://www.ncbi.nlm.nih.gov/pubmed/30065799 https://doi.org/10.30466/VRF.2018.30826 en eng Urmia University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047580/ http://www.ncbi.nlm.nih.gov/pubmed/30065799 http://dx.doi.org/10.30466/VRF.2018.30826 © 2018 Urmia University. All rights reserved. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. CC-BY-NC Original Article Text 2018 ftpubmed https://doi.org/10.30466/VRF.2018.30826 2018-08-05T00:25:15Z The purpose of the present study was to isolate and identify the pathogenic agents in Acipenser stellatus (Pallas, 1771) and Huso huso, (Linnaeus, 1758) reared in the south of Fars province, Iran which have shown infectious disease signs. Samples from spleen and kidney of 32 fishes showing septicemia symptoms such as decreasing of appetite, unbalanced swimming, expanded wounds, and petechia on the body surfaces, pectoral fins rot, visceral hemorrhage, bleeding on the spleen, and heart ascites were collected. Then samples were cultured on brain heart infusion agar growth media, stain and biological and biochemical tests on purified bacteria were performed. On the other hand, 16S rDNA region of the isolated organism was amplified using PCR. The amplified gene fragment was sequenced and evolutionary history was inferred by phylogenetic tree construction using neighbor-joining method. Results indicated that two bacterial species including Chryseobacterium joostei which isolated from the kidney of stellate sturgeon (43.00%), and Aeromonas veronii which isolated from the spleen of both sturgeon species (75.00% and 31.00% from beluga and stellate sturgeon, respectively), were recognized. Phylogenetic tree analysis showed that Fars isolated organisms including A. veronii and C. joostei had highest similarity with A. veronii bv veronii and C. joostei isolated from France, respectively. Text Beluga Beluga* PubMed Central (PMC) |
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Original Article Gholamhosseini, Amin Taghadosi, Vahideh Shiry, Nima Akhlaghi, Mostafa Sharifiyazdi, Hassan Soltanian, Siyavash Ahmadi, Nasrollah First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran |
topic_facet |
Original Article |
description |
The purpose of the present study was to isolate and identify the pathogenic agents in Acipenser stellatus (Pallas, 1771) and Huso huso, (Linnaeus, 1758) reared in the south of Fars province, Iran which have shown infectious disease signs. Samples from spleen and kidney of 32 fishes showing septicemia symptoms such as decreasing of appetite, unbalanced swimming, expanded wounds, and petechia on the body surfaces, pectoral fins rot, visceral hemorrhage, bleeding on the spleen, and heart ascites were collected. Then samples were cultured on brain heart infusion agar growth media, stain and biological and biochemical tests on purified bacteria were performed. On the other hand, 16S rDNA region of the isolated organism was amplified using PCR. The amplified gene fragment was sequenced and evolutionary history was inferred by phylogenetic tree construction using neighbor-joining method. Results indicated that two bacterial species including Chryseobacterium joostei which isolated from the kidney of stellate sturgeon (43.00%), and Aeromonas veronii which isolated from the spleen of both sturgeon species (75.00% and 31.00% from beluga and stellate sturgeon, respectively), were recognized. Phylogenetic tree analysis showed that Fars isolated organisms including A. veronii and C. joostei had highest similarity with A. veronii bv veronii and C. joostei isolated from France, respectively. |
format |
Text |
author |
Gholamhosseini, Amin Taghadosi, Vahideh Shiry, Nima Akhlaghi, Mostafa Sharifiyazdi, Hassan Soltanian, Siyavash Ahmadi, Nasrollah |
author_facet |
Gholamhosseini, Amin Taghadosi, Vahideh Shiry, Nima Akhlaghi, Mostafa Sharifiyazdi, Hassan Soltanian, Siyavash Ahmadi, Nasrollah |
author_sort |
Gholamhosseini, Amin |
title |
First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran |
title_short |
First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran |
title_full |
First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran |
title_fullStr |
First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran |
title_full_unstemmed |
First isolation and identification of Aeromonas veronii and Chryseobacterium joostei from reared sturgeons in Fars province, Iran |
title_sort |
first isolation and identification of aeromonas veronii and chryseobacterium joostei from reared sturgeons in fars province, iran |
publisher |
Urmia University Press |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047580/ http://www.ncbi.nlm.nih.gov/pubmed/30065799 https://doi.org/10.30466/VRF.2018.30826 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6047580/ http://www.ncbi.nlm.nih.gov/pubmed/30065799 http://dx.doi.org/10.30466/VRF.2018.30826 |
op_rights |
© 2018 Urmia University. All rights reserved. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.30466/VRF.2018.30826 |
_version_ |
1766374753963081728 |