PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis
Esterases are very useful biocatalysts in industry: they hydrolyze esters and split them into a carboxylic acid and an alcohol. The psychrophilic esterase PsEst3 was obtained from Paenibacillus sp. R4, which was isolated from the active layer of the permafrost in Council, Alaska. PsEst3 was successf...
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International Union of Crystallography
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ftpubmed:oai:pubmedcentral.nih.gov:5987746 2023-05-15T15:02:32+02:00 PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis Kim, Hyun Park, Ae Kyung Lee, Jun Hyuck Shin, Seung Chul Park, Hyun Kim, Han-Woo 2018-05-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987746/ http://www.ncbi.nlm.nih.gov/pubmed/29870022 https://doi.org/10.1107/S2053230X18007525 en eng International Union of Crystallography http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987746/ http://www.ncbi.nlm.nih.gov/pubmed/29870022 http://dx.doi.org/10.1107/S2053230X18007525 © International Union of Crystallography 2018 http://journals.iucr.org/services/copyrightpolicy.html Prior permission is not required to reproduce short quotations, tables and figures from this article, provided the original authors and source are cited. For more information, see http://journals.iucr.org/services/copyrightpolicy.html.http://journals.iucr.org/services/copyrightpolicy.html Acta Crystallogr F Struct Biol Commun Research Communications Text 2018 ftpubmed https://doi.org/10.1107/S2053230X18007525 2020-06-07T00:15:05Z Esterases are very useful biocatalysts in industry: they hydrolyze esters and split them into a carboxylic acid and an alcohol. The psychrophilic esterase PsEst3 was obtained from Paenibacillus sp. R4, which was isolated from the active layer of the permafrost in Council, Alaska. PsEst3 was successfully overexpressed using a psychrophilic chaperonin co-expression system and was purified by nickel-affinity and size-exclusion chromatography. Recombinant PsEst3 was crystallized at 290 K using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 2.1 Å resolution. The crystal was determined to belong to space group P4(1)32 or P4(3)32, with unit-cell parameters a = b = c = 145.33 Å. Further crystallographic analysis needs to be conducted to investigate the structure and function of this esterase. Text Arctic permafrost Alaska PubMed Central (PMC) Arctic Acta Crystallographica Section F Structural Biology Communications 74 6 367 372 |
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Research Communications |
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Research Communications Kim, Hyun Park, Ae Kyung Lee, Jun Hyuck Shin, Seung Chul Park, Hyun Kim, Han-Woo PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis |
topic_facet |
Research Communications |
description |
Esterases are very useful biocatalysts in industry: they hydrolyze esters and split them into a carboxylic acid and an alcohol. The psychrophilic esterase PsEst3 was obtained from Paenibacillus sp. R4, which was isolated from the active layer of the permafrost in Council, Alaska. PsEst3 was successfully overexpressed using a psychrophilic chaperonin co-expression system and was purified by nickel-affinity and size-exclusion chromatography. Recombinant PsEst3 was crystallized at 290 K using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 2.1 Å resolution. The crystal was determined to belong to space group P4(1)32 or P4(3)32, with unit-cell parameters a = b = c = 145.33 Å. Further crystallographic analysis needs to be conducted to investigate the structure and function of this esterase. |
format |
Text |
author |
Kim, Hyun Park, Ae Kyung Lee, Jun Hyuck Shin, Seung Chul Park, Hyun Kim, Han-Woo |
author_facet |
Kim, Hyun Park, Ae Kyung Lee, Jun Hyuck Shin, Seung Chul Park, Hyun Kim, Han-Woo |
author_sort |
Kim, Hyun |
title |
PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis |
title_short |
PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis |
title_full |
PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis |
title_fullStr |
PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis |
title_full_unstemmed |
PsEst3, a new psychrophilic esterase from the Arctic bacterium Paenibacillus sp. R4: crystallization and X-ray crystallographic analysis |
title_sort |
psest3, a new psychrophilic esterase from the arctic bacterium paenibacillus sp. r4: crystallization and x-ray crystallographic analysis |
publisher |
International Union of Crystallography |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987746/ http://www.ncbi.nlm.nih.gov/pubmed/29870022 https://doi.org/10.1107/S2053230X18007525 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic permafrost Alaska |
genre_facet |
Arctic permafrost Alaska |
op_source |
Acta Crystallogr F Struct Biol Commun |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987746/ http://www.ncbi.nlm.nih.gov/pubmed/29870022 http://dx.doi.org/10.1107/S2053230X18007525 |
op_rights |
© International Union of Crystallography 2018 http://journals.iucr.org/services/copyrightpolicy.html Prior permission is not required to reproduce short quotations, tables and figures from this article, provided the original authors and source are cited. For more information, see http://journals.iucr.org/services/copyrightpolicy.html.http://journals.iucr.org/services/copyrightpolicy.html |
op_doi |
https://doi.org/10.1107/S2053230X18007525 |
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Acta Crystallographica Section F Structural Biology Communications |
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74 |
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6 |
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367 |
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372 |
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