Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group
The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compoun...
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ftpubmed:oai:pubmedcentral.nih.gov:5983294 2023-05-15T14:52:34+02:00 Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette Hammer Gerwick, William H. Hansen, Espen 2018-05-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983294/ http://www.ncbi.nlm.nih.gov/pubmed/29758007 https://doi.org/10.3390/md16050163 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983294/ http://www.ncbi.nlm.nih.gov/pubmed/29758007 http://dx.doi.org/10.3390/md16050163 © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2018 ftpubmed https://doi.org/10.3390/md16050163 2018-06-10T00:26:37Z The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compounds. In the present work, an Arctic marine Pseudomonas sp. belonging to the Pseudomonas (P.) fluorescence group was cultivated in four different media in an attempt to activate biosynthetic pathways leading to the production of antibacterial and anticancer compounds. Culture extracts were pre-fractionated and screened for antibacterial and anticancer activities. One fraction from three of the four growth conditions showed inhibitory activity towards bacteria and cancer cells. The active fractions were dereplicated using molecular networking based on MS/MS fragmentation data, indicating the presence of a cluster of related rhamnolipids. Six compounds were isolated using HPLC and mass-guided fractionation, and by interpreting data from NMR and high-resolution MS/MS analysis; the structures of the compounds were determined to be five mono-rhamnolipids and the lipid moiety of one of the rhamnolipids. Molecular networking proved to be a valuable tool for dereplication of these related compounds, and for the first time, five mono-rhamnolipids from a bacterium within the P. fluorescence group were characterized, including one new mono-rhamnolipid. Text Arctic PubMed Central (PMC) Arctic Marine Drugs 16 5 163 |
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Article Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette Hammer Gerwick, William H. Hansen, Espen Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
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description |
The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compounds. In the present work, an Arctic marine Pseudomonas sp. belonging to the Pseudomonas (P.) fluorescence group was cultivated in four different media in an attempt to activate biosynthetic pathways leading to the production of antibacterial and anticancer compounds. Culture extracts were pre-fractionated and screened for antibacterial and anticancer activities. One fraction from three of the four growth conditions showed inhibitory activity towards bacteria and cancer cells. The active fractions were dereplicated using molecular networking based on MS/MS fragmentation data, indicating the presence of a cluster of related rhamnolipids. Six compounds were isolated using HPLC and mass-guided fractionation, and by interpreting data from NMR and high-resolution MS/MS analysis; the structures of the compounds were determined to be five mono-rhamnolipids and the lipid moiety of one of the rhamnolipids. Molecular networking proved to be a valuable tool for dereplication of these related compounds, and for the first time, five mono-rhamnolipids from a bacterium within the P. fluorescence group were characterized, including one new mono-rhamnolipid. |
format |
Text |
author |
Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette Hammer Gerwick, William H. Hansen, Espen |
author_facet |
Kristoffersen, Venke Rämä, Teppo Isaksson, Johan Andersen, Jeanette Hammer Gerwick, William H. Hansen, Espen |
author_sort |
Kristoffersen, Venke |
title |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_short |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_full |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_fullStr |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_full_unstemmed |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_sort |
characterization of rhamnolipids produced by an arctic marine bacterium from the pseudomonas fluorescence group |
publisher |
MDPI |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983294/ http://www.ncbi.nlm.nih.gov/pubmed/29758007 https://doi.org/10.3390/md16050163 |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5983294/ http://www.ncbi.nlm.nih.gov/pubmed/29758007 http://dx.doi.org/10.3390/md16050163 |
op_rights |
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/md16050163 |
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Marine Drugs |
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16 |
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5 |
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163 |
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1766323800223252480 |