Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota
The microbiome is essential for development, health and homeostasis throughout an animal’s life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of...
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ftpubmed:oai:pubmedcentral.nih.gov:5932060 2023-05-15T13:10:06+02:00 Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota van Veelen, H. Pieter J. Salles, Joana Falcão Tieleman, B. Irene 2018-02-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932060/ http://www.ncbi.nlm.nih.gov/pubmed/29445132 https://doi.org/10.1038/s41396-018-0067-3 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932060/ http://www.ncbi.nlm.nih.gov/pubmed/29445132 http://dx.doi.org/10.1038/s41396-018-0067-3 © International Society for Microbial Ecology 2018 Article Text 2018 ftpubmed https://doi.org/10.1038/s41396-018-0067-3 2019-05-05T00:13:55Z The microbiome is essential for development, health and homeostasis throughout an animal’s life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of the maternal microbiome, warranting characterisation of egg microbiome assembly. Here, we investigated maternal and environmental contributions to avian eggshell microbiota in wild passerine birds: woodlark Lullula arborea and skylark Alauda arvensis. Using 16S rRNA gene sequencing, we demonstrated in both lark species, at the population and within-nest levels, that bacterial communities of freshly laid eggs were distinct from the female cloacal microbiome. Instead, soil-borne bacteria appeared to thrive on freshly laid eggs, and eggshell microbiota composition strongly resembled maternal skin, body feather and nest material communities, sources in direct contact with laid eggs. Finally, phylogenetic structure analysis and microbial source tracking underscored species sorting from directly contacting sources rather than in vivo-transferred symbionts. The female-egg-nest system allowed an integrative assessment of avian egg microbiome assembly, revealing mixed modes of symbiont acquisition not previously documented for vertebrate eggs. Our findings illuminated egg microbiome origins, which suggested a limited potential of eggshells for transgenerational transmission, encouraging further investigation of eggshell microbiome functions in vertebrates. Text Alauda arvensis PubMed Central (PMC) The ISME Journal 12 5 1375 1388 |
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Article van Veelen, H. Pieter J. Salles, Joana Falcão Tieleman, B. Irene Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
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description |
The microbiome is essential for development, health and homeostasis throughout an animal’s life. Yet, the origins and transmission processes governing animal microbiomes remain elusive for non-human vertebrates, oviparous vertebrates in particular. Eggs may function as transgenerational carriers of the maternal microbiome, warranting characterisation of egg microbiome assembly. Here, we investigated maternal and environmental contributions to avian eggshell microbiota in wild passerine birds: woodlark Lullula arborea and skylark Alauda arvensis. Using 16S rRNA gene sequencing, we demonstrated in both lark species, at the population and within-nest levels, that bacterial communities of freshly laid eggs were distinct from the female cloacal microbiome. Instead, soil-borne bacteria appeared to thrive on freshly laid eggs, and eggshell microbiota composition strongly resembled maternal skin, body feather and nest material communities, sources in direct contact with laid eggs. Finally, phylogenetic structure analysis and microbial source tracking underscored species sorting from directly contacting sources rather than in vivo-transferred symbionts. The female-egg-nest system allowed an integrative assessment of avian egg microbiome assembly, revealing mixed modes of symbiont acquisition not previously documented for vertebrate eggs. Our findings illuminated egg microbiome origins, which suggested a limited potential of eggshells for transgenerational transmission, encouraging further investigation of eggshell microbiome functions in vertebrates. |
format |
Text |
author |
van Veelen, H. Pieter J. Salles, Joana Falcão Tieleman, B. Irene |
author_facet |
van Veelen, H. Pieter J. Salles, Joana Falcão Tieleman, B. Irene |
author_sort |
van Veelen, H. Pieter J. |
title |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_short |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_full |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_fullStr |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_full_unstemmed |
Microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
title_sort |
microbiome assembly of avian eggshells and their potential as transgenerational carriers of maternal microbiota |
publisher |
Nature Publishing Group UK |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932060/ http://www.ncbi.nlm.nih.gov/pubmed/29445132 https://doi.org/10.1038/s41396-018-0067-3 |
genre |
Alauda arvensis |
genre_facet |
Alauda arvensis |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5932060/ http://www.ncbi.nlm.nih.gov/pubmed/29445132 http://dx.doi.org/10.1038/s41396-018-0067-3 |
op_rights |
© International Society for Microbial Ecology 2018 |
op_doi |
https://doi.org/10.1038/s41396-018-0067-3 |
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The ISME Journal |
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12 |
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5 |
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1375 |
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1388 |
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1766215276975620096 |