Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model
Parkinson's disease (PD) is a commonly reported age-related neurodegenerative disorder. Microglial-mediated neuroinflammation is one of the cardinal hallmarks of various neurodegenerative disorders, including PD progression. Inadequate therapeutic strategies and substantial adverse effects of w...
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ftpubmed:oai:pubmedcentral.nih.gov:5925162 2023-05-15T16:01:20+02:00 Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model Park, Shin-Young Karthivashan, Govindarajan Ko, Hyun Myung Cho, Duk-Yeon Kim, Joonsoo Cho, Dae Jun Ganesan, Palanivel Su-Kim, In Choi, Dong-Kug 2018-04-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925162/ https://doi.org/10.1155/2018/3175214 en eng Hindawi http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925162/ http://dx.doi.org/10.1155/2018/3175214 Copyright © 2018 Shin-Young Park et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2018 ftpubmed https://doi.org/10.1155/2018/3175214 2018-06-03T00:23:05Z Parkinson's disease (PD) is a commonly reported age-related neurodegenerative disorder. Microglial-mediated neuroinflammation is one of the cardinal hallmarks of various neurodegenerative disorders, including PD progression. Inadequate therapeutic strategies and substantial adverse effects of well-established drug candidates demand new therapeutic leads to treat PD. Dendropanax morbifera (DM) is an endemic plant species of South Korea, and it has been used extensively as traditional medicine to treat numerous clinical complications. In this study, we conducted an initial profiling of the few major phytoconstituents of aqueous DM leaf extracts (DML) and quantified the same using high-performance liquid chromatography tandem mass spectrometry with electrospray ionization (HPLC-ESI-MS/MS). We subsequently evaluated the antineuroinflammatory activity and ameliorative potential of DML in both in vitro and in vivo experimental PD models. The prophylactic treatment of DML effectually improved the behavioral deficits, curbed the microglial-mediated neuroinflammation, and protected dopaminergic (DA) neuronal loss by restoring tyrosine hydroxylase (TH) levels in brain tissue of the MPTP-induced PD mouse model. We conducted chromatographic profiling and identified chlorogenic acid (CA) as a major constituent (19.5 mg/g of BuOH fraction), which has been well documented as an antioxidant and anti-inflammatory agent. This was found to be in harmony with our in vitro results, where DML suppressed the level of inflammatory mediators and allied the signaling pathway in LPS-stimulated microglial cells. The results of our study indicate that DML and its bioactive constituents can be developed as potential therapeutic candidates against progressive PD complications. Text DML PubMed Central (PMC) Oxidative Medicine and Cellular Longevity 2018 1 14 |
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Research Article Park, Shin-Young Karthivashan, Govindarajan Ko, Hyun Myung Cho, Duk-Yeon Kim, Joonsoo Cho, Dae Jun Ganesan, Palanivel Su-Kim, In Choi, Dong-Kug Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model |
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Research Article |
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Parkinson's disease (PD) is a commonly reported age-related neurodegenerative disorder. Microglial-mediated neuroinflammation is one of the cardinal hallmarks of various neurodegenerative disorders, including PD progression. Inadequate therapeutic strategies and substantial adverse effects of well-established drug candidates demand new therapeutic leads to treat PD. Dendropanax morbifera (DM) is an endemic plant species of South Korea, and it has been used extensively as traditional medicine to treat numerous clinical complications. In this study, we conducted an initial profiling of the few major phytoconstituents of aqueous DM leaf extracts (DML) and quantified the same using high-performance liquid chromatography tandem mass spectrometry with electrospray ionization (HPLC-ESI-MS/MS). We subsequently evaluated the antineuroinflammatory activity and ameliorative potential of DML in both in vitro and in vivo experimental PD models. The prophylactic treatment of DML effectually improved the behavioral deficits, curbed the microglial-mediated neuroinflammation, and protected dopaminergic (DA) neuronal loss by restoring tyrosine hydroxylase (TH) levels in brain tissue of the MPTP-induced PD mouse model. We conducted chromatographic profiling and identified chlorogenic acid (CA) as a major constituent (19.5 mg/g of BuOH fraction), which has been well documented as an antioxidant and anti-inflammatory agent. This was found to be in harmony with our in vitro results, where DML suppressed the level of inflammatory mediators and allied the signaling pathway in LPS-stimulated microglial cells. The results of our study indicate that DML and its bioactive constituents can be developed as potential therapeutic candidates against progressive PD complications. |
format |
Text |
author |
Park, Shin-Young Karthivashan, Govindarajan Ko, Hyun Myung Cho, Duk-Yeon Kim, Joonsoo Cho, Dae Jun Ganesan, Palanivel Su-Kim, In Choi, Dong-Kug |
author_facet |
Park, Shin-Young Karthivashan, Govindarajan Ko, Hyun Myung Cho, Duk-Yeon Kim, Joonsoo Cho, Dae Jun Ganesan, Palanivel Su-Kim, In Choi, Dong-Kug |
author_sort |
Park, Shin-Young |
title |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model |
title_short |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model |
title_full |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model |
title_fullStr |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model |
title_full_unstemmed |
Aqueous Extract of Dendropanax morbiferus Leaves Effectively Alleviated Neuroinflammation and Behavioral Impediments in MPTP-Induced Parkinson's Mouse Model |
title_sort |
aqueous extract of dendropanax morbiferus leaves effectively alleviated neuroinflammation and behavioral impediments in mptp-induced parkinson's mouse model |
publisher |
Hindawi |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925162/ https://doi.org/10.1155/2018/3175214 |
genre |
DML |
genre_facet |
DML |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5925162/ http://dx.doi.org/10.1155/2018/3175214 |
op_rights |
Copyright © 2018 Shin-Young Park et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1155/2018/3175214 |
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Oxidative Medicine and Cellular Longevity |
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2018 |
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1 |
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14 |
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1766397245594271744 |