Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently eme...
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ftpubmed:oai:pubmedcentral.nih.gov:5923313 2023-05-15T14:01:14+02:00 Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse IV, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick 2018-04-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923313/ http://www.ncbi.nlm.nih.gov/pubmed/29614044 https://doi.org/10.3390/toxins10040147 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923313/ http://www.ncbi.nlm.nih.gov/pubmed/29614044 http://dx.doi.org/10.3390/toxins10040147 © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2018 ftpubmed https://doi.org/10.3390/toxins10040147 2018-05-06T00:36:19Z Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp3, ADMAdda5, Dhb7] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen. Text Antarc* Antarctic Arctic Svalbard PubMed Central (PMC) Antarctic Arctic Svalbard Svalbard Archipelago Toxins 10 4 147 |
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Article Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse IV, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
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description |
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp3, ADMAdda5, Dhb7] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen. |
format |
Text |
author |
Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse IV, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick |
author_facet |
Kleinteich, Julia Puddick, Jonathan Wood, Susanna A. Hildebrand, Falk Laughinghouse IV, H. Dail Pearce, David A. Dietrich, Daniel R. Wilmotte, Annick |
author_sort |
Kleinteich, Julia |
title |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_short |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_full |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_fullStr |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_full_unstemmed |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_sort |
toxic cyanobacteria in svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
publisher |
MDPI |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923313/ http://www.ncbi.nlm.nih.gov/pubmed/29614044 https://doi.org/10.3390/toxins10040147 |
geographic |
Antarctic Arctic Svalbard Svalbard Archipelago |
geographic_facet |
Antarctic Arctic Svalbard Svalbard Archipelago |
genre |
Antarc* Antarctic Arctic Svalbard |
genre_facet |
Antarc* Antarctic Arctic Svalbard |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5923313/ http://www.ncbi.nlm.nih.gov/pubmed/29614044 http://dx.doi.org/10.3390/toxins10040147 |
op_rights |
© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/toxins10040147 |
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Toxins |
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147 |
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