Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm
Enchytraeus albidus is a terrestrial earthworm widespread along the coasts of northern Europe and the Arctic. This species tolerates freezing of body fluids and survives winters in a frozen state. Their acclimatory physiological mechanisms behind freeze tolerance involve increased fluidity of membra...
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ftpubmed:oai:pubmedcentral.nih.gov:5901168 2023-05-15T15:12:33+02:00 Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm de Boer, Tjalf E. Roelofs, Dick Vooijs, Riet Holmstrup, Martin Amorim, Mónica J. B. 2018-03-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5901168/ http://www.ncbi.nlm.nih.gov/pubmed/29686857 https://doi.org/10.1002/ece3.3602 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5901168/ http://www.ncbi.nlm.nih.gov/pubmed/29686857 http://dx.doi.org/10.1002/ece3.3602 © 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Original Research Text 2018 ftpubmed https://doi.org/10.1002/ece3.3602 2018-04-29T00:10:27Z Enchytraeus albidus is a terrestrial earthworm widespread along the coasts of northern Europe and the Arctic. This species tolerates freezing of body fluids and survives winters in a frozen state. Their acclimatory physiological mechanisms behind freeze tolerance involve increased fluidity of membrane lipids during cold exposure and accumulation of cryoprotectants (glucose) during the freezing process. Gene regulatory processes of these physiological responses have not been studied, partly because no gene expression tools were developed. The main aim of this study was to understand whether the freeze tolerance mechanisms have a transcriptomic basis in E. albidus. For that purpose, first the transcriptome of E. albidus was assembled with RNAseq data. Second, two strains from contrasting thermal environments (Germany and Greenland) were compared by mapping barcoded RNAseq data onto the assembled transcriptome. Both of these strains are freeze tolerant, but Greenland is extremely freeze tolerant. Results showed more plastic responses in the Greenland strain as well as higher constitutive expression of particular stress response genes. These altered transcriptional networks are associated with an adapted homeostasis coping with prolonged freezing conditions in Greenland animals. Previously identified physiological alterations in freeze‐tolerant strains of E. albidus are underpinned at the transcriptome level. These processes involve anion transport in the hemolymph, fatty acid metabolism, metabolism, and transport of cryoprotective sugars as well as protection against oxidative stress. Pathway analysis supported most of these processes, and identified additional differentially expressed pathways such as peroxisome and Toll‐like receptor signaling. We propose that the freeze‐tolerant phenotype is the consequence of genetic adaptation to cold stress and may have driven evolutionary divergence of the two strains. Text Arctic Greenland PubMed Central (PMC) Arctic Greenland Ecology and Evolution 8 7 3774 3786 |
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Original Research |
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Original Research de Boer, Tjalf E. Roelofs, Dick Vooijs, Riet Holmstrup, Martin Amorim, Mónica J. B. Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm |
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Original Research |
description |
Enchytraeus albidus is a terrestrial earthworm widespread along the coasts of northern Europe and the Arctic. This species tolerates freezing of body fluids and survives winters in a frozen state. Their acclimatory physiological mechanisms behind freeze tolerance involve increased fluidity of membrane lipids during cold exposure and accumulation of cryoprotectants (glucose) during the freezing process. Gene regulatory processes of these physiological responses have not been studied, partly because no gene expression tools were developed. The main aim of this study was to understand whether the freeze tolerance mechanisms have a transcriptomic basis in E. albidus. For that purpose, first the transcriptome of E. albidus was assembled with RNAseq data. Second, two strains from contrasting thermal environments (Germany and Greenland) were compared by mapping barcoded RNAseq data onto the assembled transcriptome. Both of these strains are freeze tolerant, but Greenland is extremely freeze tolerant. Results showed more plastic responses in the Greenland strain as well as higher constitutive expression of particular stress response genes. These altered transcriptional networks are associated with an adapted homeostasis coping with prolonged freezing conditions in Greenland animals. Previously identified physiological alterations in freeze‐tolerant strains of E. albidus are underpinned at the transcriptome level. These processes involve anion transport in the hemolymph, fatty acid metabolism, metabolism, and transport of cryoprotective sugars as well as protection against oxidative stress. Pathway analysis supported most of these processes, and identified additional differentially expressed pathways such as peroxisome and Toll‐like receptor signaling. We propose that the freeze‐tolerant phenotype is the consequence of genetic adaptation to cold stress and may have driven evolutionary divergence of the two strains. |
format |
Text |
author |
de Boer, Tjalf E. Roelofs, Dick Vooijs, Riet Holmstrup, Martin Amorim, Mónica J. B. |
author_facet |
de Boer, Tjalf E. Roelofs, Dick Vooijs, Riet Holmstrup, Martin Amorim, Mónica J. B. |
author_sort |
de Boer, Tjalf E. |
title |
Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm |
title_short |
Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm |
title_full |
Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm |
title_fullStr |
Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm |
title_full_unstemmed |
Population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm |
title_sort |
population‐specific transcriptional differences associated with freeze tolerance in a terrestrial worm |
publisher |
John Wiley and Sons Inc. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5901168/ http://www.ncbi.nlm.nih.gov/pubmed/29686857 https://doi.org/10.1002/ece3.3602 |
geographic |
Arctic Greenland |
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Arctic Greenland |
genre |
Arctic Greenland |
genre_facet |
Arctic Greenland |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5901168/ http://www.ncbi.nlm.nih.gov/pubmed/29686857 http://dx.doi.org/10.1002/ece3.3602 |
op_rights |
© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
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CC-BY |
op_doi |
https://doi.org/10.1002/ece3.3602 |
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Ecology and Evolution |
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8 |
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7 |
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3774 |
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3786 |
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