pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas)
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and sa...
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ftpubmed:oai:pubmedcentral.nih.gov:5898264 2023-05-15T15:57:58+02:00 pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky 2018-02-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898264/ http://www.ncbi.nlm.nih.gov/pubmed/29483075 https://doi.org/10.1242/bio.031427 en eng The Company of Biologists Ltd http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898264/ http://www.ncbi.nlm.nih.gov/pubmed/29483075 http://dx.doi.org/10.1242/bio.031427 © 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. CC-BY Research Article Text 2018 ftpubmed https://doi.org/10.1242/bio.031427 2018-04-22T01:32:29Z Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82±0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Biology Open |
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Research Article Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
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Research Article |
description |
Investigating the roles of chemical factors stimulating and inhibiting sperm motility is required to understand the mechanisms of spermatozoa movement. In this study, we described the composition of the seminal fluid (osmotic pressure, pH, and ions) and investigated the roles of these factors and salinity in initiating spermatozoa movement in the Pacific oyster, Crassostrea gigas. The acidic pH of the gonad (5.82±0.22) maintained sperm in the quiescent stage and initiation of flagellar movement was triggered by a sudden increase of spermatozoa external pH (pHe) when released in seawater (SW). At pH 6.4, percentage of motile spermatozoa was three times higher when they were activated in SW containing 30 mM NH4Cl, which alkalinizes internal pH (pHi) of spermatozoa, compared to NH4Cl-free SW, revealing the role of pHi in triggering sperm movement. Percentage of motile spermatozoa activated in Na+-free artificial seawater (ASW) was highly reduced compared to ASW, suggesting that change of pHi triggering sperm motility was mediated by a Na+/H+ exchanger. Motility and swimming speed were highest in salinities between 33.8 and 42.7‰ (within a range of 0 to 50 ‰), and pH values above 7.5 (within a range of 4.5 to 9.5). |
format |
Text |
author |
Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_facet |
Boulais, Myrina Suquet, Marc Arsenault-Pernet, Eve Julie Malo, Florent Queau, Isabelle Pignet, Patricia Ratiskol, Dominique Le Grand, Jacqueline Huber, Matthias Cosson, Jacky |
author_sort |
Boulais, Myrina |
title |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_short |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_fullStr |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_full_unstemmed |
pH controls spermatozoa motility in the Pacific oyster (Crassostrea gigas) |
title_sort |
ph controls spermatozoa motility in the pacific oyster (crassostrea gigas) |
publisher |
The Company of Biologists Ltd |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898264/ http://www.ncbi.nlm.nih.gov/pubmed/29483075 https://doi.org/10.1242/bio.031427 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5898264/ http://www.ncbi.nlm.nih.gov/pubmed/29483075 http://dx.doi.org/10.1242/bio.031427 |
op_rights |
© 2018. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1242/bio.031427 |
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Biology Open |
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1766393682895831040 |