Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria
Information on mosquito-borne filarioid helminths in Austria is scarce, but recent discoveries of Dirofilaria repens indicate autochthonous distribution of this parasite in Eastern Austria. In the current xenomonitoring study, more than 48,000 mosquitoes were collected in Eastern Austria between 201...
Published in: | Canadian Journal of Infectious Diseases and Medical Microbiology |
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ftpubmed:oai:pubmedcentral.nih.gov:5875040 2023-05-15T18:40:09+02:00 Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria Übleis, Sarah Susanne Cuk, Claudia Nawratil, Michaela Butter, Julia Schoener, Ellen Obwaller, Adelheid G. Zechmeister, Thomas Duscher, Georg G. Rubel, Franz Lebl, Karin Zittra, Carina Fuehrer, Hans-Peter 2018-03-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875040/ http://www.ncbi.nlm.nih.gov/pubmed/29736197 https://doi.org/10.1155/2018/9754695 en eng Hindawi http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875040/ http://www.ncbi.nlm.nih.gov/pubmed/29736197 http://dx.doi.org/10.1155/2018/9754695 Copyright © 2018 Sarah Susanne Übleis et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY Research Article Text 2018 ftpubmed https://doi.org/10.1155/2018/9754695 2018-05-13T00:08:18Z Information on mosquito-borne filarioid helminths in Austria is scarce, but recent discoveries of Dirofilaria repens indicate autochthonous distribution of this parasite in Eastern Austria. In the current xenomonitoring study, more than 48,000 mosquitoes were collected in Eastern Austria between 2013 and 2015, using different sampling techniques and storage conditions, and were analysed in pools with molecular tools for the presence of filarioid helminth DNA. Overall, DNA of D. repens, Setaria tundra, and two unknown filarioid helminths were documented in twenty mosquito pools within the mitochondrial cox1 gene (barcode region). These results indicate that S. tundra, with roe deer as definite hosts, is common in Eastern Austria, with most occurrences in floodplain mosquitoes (e.g., Aedes vexans). Moreover, DNA of D. repens was found in an Anopheles plumbeus mosquito close to the Slovakian border, indicating that D. repens is endemic in low prevalence in Eastern Austria. This study shows that xenomonitoring is an adequate tool to analyse the presence of filarioid helminths, but results are influenced by mosquito sampling techniques, storage conditions, and molecular protocols. Text Tundra PubMed Central (PMC) Canadian Journal of Infectious Diseases and Medical Microbiology 2018 1 6 |
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Research Article Übleis, Sarah Susanne Cuk, Claudia Nawratil, Michaela Butter, Julia Schoener, Ellen Obwaller, Adelheid G. Zechmeister, Thomas Duscher, Georg G. Rubel, Franz Lebl, Karin Zittra, Carina Fuehrer, Hans-Peter Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria |
topic_facet |
Research Article |
description |
Information on mosquito-borne filarioid helminths in Austria is scarce, but recent discoveries of Dirofilaria repens indicate autochthonous distribution of this parasite in Eastern Austria. In the current xenomonitoring study, more than 48,000 mosquitoes were collected in Eastern Austria between 2013 and 2015, using different sampling techniques and storage conditions, and were analysed in pools with molecular tools for the presence of filarioid helminth DNA. Overall, DNA of D. repens, Setaria tundra, and two unknown filarioid helminths were documented in twenty mosquito pools within the mitochondrial cox1 gene (barcode region). These results indicate that S. tundra, with roe deer as definite hosts, is common in Eastern Austria, with most occurrences in floodplain mosquitoes (e.g., Aedes vexans). Moreover, DNA of D. repens was found in an Anopheles plumbeus mosquito close to the Slovakian border, indicating that D. repens is endemic in low prevalence in Eastern Austria. This study shows that xenomonitoring is an adequate tool to analyse the presence of filarioid helminths, but results are influenced by mosquito sampling techniques, storage conditions, and molecular protocols. |
format |
Text |
author |
Übleis, Sarah Susanne Cuk, Claudia Nawratil, Michaela Butter, Julia Schoener, Ellen Obwaller, Adelheid G. Zechmeister, Thomas Duscher, Georg G. Rubel, Franz Lebl, Karin Zittra, Carina Fuehrer, Hans-Peter |
author_facet |
Übleis, Sarah Susanne Cuk, Claudia Nawratil, Michaela Butter, Julia Schoener, Ellen Obwaller, Adelheid G. Zechmeister, Thomas Duscher, Georg G. Rubel, Franz Lebl, Karin Zittra, Carina Fuehrer, Hans-Peter |
author_sort |
Übleis, Sarah Susanne |
title |
Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria |
title_short |
Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria |
title_full |
Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria |
title_fullStr |
Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria |
title_full_unstemmed |
Xenomonitoring of Mosquitoes (Diptera: Culicidae) for the Presence of Filarioid Helminths in Eastern Austria |
title_sort |
xenomonitoring of mosquitoes (diptera: culicidae) for the presence of filarioid helminths in eastern austria |
publisher |
Hindawi |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875040/ http://www.ncbi.nlm.nih.gov/pubmed/29736197 https://doi.org/10.1155/2018/9754695 |
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Tundra |
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Tundra |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875040/ http://www.ncbi.nlm.nih.gov/pubmed/29736197 http://dx.doi.org/10.1155/2018/9754695 |
op_rights |
Copyright © 2018 Sarah Susanne Übleis et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1155/2018/9754695 |
container_title |
Canadian Journal of Infectious Diseases and Medical Microbiology |
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2018 |
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1 |
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6 |
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1766229355477860352 |