A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas
Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we design...
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ftpubmed:oai:pubmedcentral.nih.gov:5868073 2023-05-15T15:57:53+02:00 A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas Wu, Changlu Jiang, Qiuyun Wei, Lei Cai, Zhongqiang Chen, Jun Yu, Wenchao He, Cheng Wang, Jiao Guo, Wen Wang, Xiaotong 2018-03-19 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868073/ https://doi.org/10.3389/fphys.2018.00221 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868073/ http://dx.doi.org/10.3389/fphys.2018.00221 Copyright © 2018 Wu, Jiang, Wei, Cai, Chen, Yu, He, Wang, Guo and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Physiology Text 2018 ftpubmed https://doi.org/10.3389/fphys.2018.00221 2018-04-08T00:36:24Z Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we designed experiments to identify the genes associated with light-sensitivity in adult oysters. First, we assessed the Pacific oyster genome and identified 368 genes annotated with the terms associated with light-sensitivity. Second, the function of the four rhodopsin-like superfamily member genes was tested by using RNAi. The results showed that the highest level of mRNA expression of the vision-related genes was in the mantle; however, this finding is not true for all oyster genes. Interestingly, we also found four rhodopsin-like superfamily member genes expressed at an very high level in the mantle tissue. In the RNAi experiment, when one of rhodopsin-like superfamily member genes (CGI_1001253) was inhibited, the light-sensitivity capacity of the injected oysters was significantly reduced, suggesting that CGI_10012534 may be associated with light-sensitivity in the adult Pacific oyster. Text Crassostrea gigas Pacific oyster PubMed Central (PMC) Pacific Frontiers in Physiology 9 |
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Physiology |
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Physiology Wu, Changlu Jiang, Qiuyun Wei, Lei Cai, Zhongqiang Chen, Jun Yu, Wenchao He, Cheng Wang, Jiao Guo, Wen Wang, Xiaotong A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
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Physiology |
description |
Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we designed experiments to identify the genes associated with light-sensitivity in adult oysters. First, we assessed the Pacific oyster genome and identified 368 genes annotated with the terms associated with light-sensitivity. Second, the function of the four rhodopsin-like superfamily member genes was tested by using RNAi. The results showed that the highest level of mRNA expression of the vision-related genes was in the mantle; however, this finding is not true for all oyster genes. Interestingly, we also found four rhodopsin-like superfamily member genes expressed at an very high level in the mantle tissue. In the RNAi experiment, when one of rhodopsin-like superfamily member genes (CGI_1001253) was inhibited, the light-sensitivity capacity of the injected oysters was significantly reduced, suggesting that CGI_10012534 may be associated with light-sensitivity in the adult Pacific oyster. |
format |
Text |
author |
Wu, Changlu Jiang, Qiuyun Wei, Lei Cai, Zhongqiang Chen, Jun Yu, Wenchao He, Cheng Wang, Jiao Guo, Wen Wang, Xiaotong |
author_facet |
Wu, Changlu Jiang, Qiuyun Wei, Lei Cai, Zhongqiang Chen, Jun Yu, Wenchao He, Cheng Wang, Jiao Guo, Wen Wang, Xiaotong |
author_sort |
Wu, Changlu |
title |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_short |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_full |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_fullStr |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas |
title_sort |
rhodopsin-like gene may be associated with the light-sensitivity of adult pacific oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868073/ https://doi.org/10.3389/fphys.2018.00221 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5868073/ http://dx.doi.org/10.3389/fphys.2018.00221 |
op_rights |
Copyright © 2018 Wu, Jiang, Wei, Cai, Chen, Yu, He, Wang, Guo and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2018.00221 |
container_title |
Frontiers in Physiology |
container_volume |
9 |
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1766393588118192128 |