Development and characterization of two cell lines from gills of Atlantic salmon
Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines fro...
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ftpubmed:oai:pubmedcentral.nih.gov:5812586 2023-05-15T15:28:31+02:00 Development and characterization of two cell lines from gills of Atlantic salmon Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. 2018-02-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812586/ http://www.ncbi.nlm.nih.gov/pubmed/29444101 https://doi.org/10.1371/journal.pone.0191792 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812586/ http://www.ncbi.nlm.nih.gov/pubmed/29444101 http://dx.doi.org/10.1371/journal.pone.0191792 https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. CC0 PDM Research Article Text 2018 ftpubmed https://doi.org/10.1371/journal.pone.0191792 2018-03-04T01:30:27Z Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cytokeratin and e-cadherin and with desmosomes as seen by transmission electron microscopy suggesting the cells to be of epithelial origin. These structures were not seen in ASG-13. The cell lines have been maintained for almost 30 passages and both cell lines are fully susceptible to infection by infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), Atlantic salmon reovirus TS (TSRV) and Pacific salmon paramyxovirus (PSPV). While infectious salmon anemia virus (ISAV) did not cause visible CPE, immunofluorescent staining revealed a sub-fraction of cells in both the ASG-10 and ASG-13 lines may be permissive to infection. ASG-10 is able to proliferate and migrate to close scratches in the monolayer within seven days in vitro contrary to ASG-13, which does not appear to do have the same proliferative and migratory ability. These cell lines will be useful in studies of gill diseases in Atlantic salmon and may represent an important contribution for alternatives to experimental animals and studies of epithelial–mesenchymal cell biology. Text Atlantic salmon Salmo salar PubMed Central (PMC) Pacific PLOS ONE 13 2 e0191792 |
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Research Article Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. Development and characterization of two cell lines from gills of Atlantic salmon |
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Research Article |
description |
Gill disease in Atlantic salmon, Salmo salar L., causes big losses in the salmon farming industry. Until now, tools to cultivate microorganisms causing gill disease and models to study the gill responses have been lacking. Here we describe the establishment and characterization of two cell lines from the gills of Atlantic salmon. Atlantic salmon gill cell ASG-10 consisted of cells staining for cytokeratin and e-cadherin and with desmosomes as seen by transmission electron microscopy suggesting the cells to be of epithelial origin. These structures were not seen in ASG-13. The cell lines have been maintained for almost 30 passages and both cell lines are fully susceptible to infection by infectious hematopoietic necrosis virus (IHNV), viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), Atlantic salmon reovirus TS (TSRV) and Pacific salmon paramyxovirus (PSPV). While infectious salmon anemia virus (ISAV) did not cause visible CPE, immunofluorescent staining revealed a sub-fraction of cells in both the ASG-10 and ASG-13 lines may be permissive to infection. ASG-10 is able to proliferate and migrate to close scratches in the monolayer within seven days in vitro contrary to ASG-13, which does not appear to do have the same proliferative and migratory ability. These cell lines will be useful in studies of gill diseases in Atlantic salmon and may represent an important contribution for alternatives to experimental animals and studies of epithelial–mesenchymal cell biology. |
format |
Text |
author |
Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. |
author_facet |
Gjessing, Mona C. Aamelfot, Maria Batts, William N. Benestad, Sylvie L. Dale, Ole B. Thoen, Even Weli, Simon C. Winton, James R. |
author_sort |
Gjessing, Mona C. |
title |
Development and characterization of two cell lines from gills of Atlantic salmon |
title_short |
Development and characterization of two cell lines from gills of Atlantic salmon |
title_full |
Development and characterization of two cell lines from gills of Atlantic salmon |
title_fullStr |
Development and characterization of two cell lines from gills of Atlantic salmon |
title_full_unstemmed |
Development and characterization of two cell lines from gills of Atlantic salmon |
title_sort |
development and characterization of two cell lines from gills of atlantic salmon |
publisher |
Public Library of Science |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812586/ http://www.ncbi.nlm.nih.gov/pubmed/29444101 https://doi.org/10.1371/journal.pone.0191792 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5812586/ http://www.ncbi.nlm.nih.gov/pubmed/29444101 http://dx.doi.org/10.1371/journal.pone.0191792 |
op_rights |
https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
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https://doi.org/10.1371/journal.pone.0191792 |
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PLOS ONE |
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13 |
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2 |
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e0191792 |
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