Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia
Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Ant...
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The Japanese Society of Veterinary Science
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ftpubmed:oai:pubmedcentral.nih.gov:5797873 2023-05-15T14:00:30+02:00 Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia GRILLOVÁ, Linda SEDLÁČEK, Ivo PÁCHNÍKOVÁ, Gabriela STAŇKOVÁ, Eva ŠVEC, Pavel HOLOCHOVÁ, Pavla MICENKOVÁ, Lenka BOSÁK, Juraj SLANINOVÁ, Iva ŠMAJS, David 2017-12-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797873/ http://www.ncbi.nlm.nih.gov/pubmed/29249728 https://doi.org/10.1292/jvms.17-0492 en eng The Japanese Society of Veterinary Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797873/ http://www.ncbi.nlm.nih.gov/pubmed/29249728 http://dx.doi.org/10.1292/jvms.17-0492 ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) CC-BY-NC-ND Bacteriology Text 2017 ftpubmed https://doi.org/10.1292/jvms.17-0492 2018-02-11T01:25:52Z Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity. Text Antarc* Antarctica James Ross Island Ross Island PubMed Central (PMC) Patagonia Ross Island Journal of Veterinary Medical Science 80 1 138 146 |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Bacteriology |
spellingShingle |
Bacteriology GRILLOVÁ, Linda SEDLÁČEK, Ivo PÁCHNÍKOVÁ, Gabriela STAŇKOVÁ, Eva ŠVEC, Pavel HOLOCHOVÁ, Pavla MICENKOVÁ, Lenka BOSÁK, Juraj SLANINOVÁ, Iva ŠMAJS, David Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia |
topic_facet |
Bacteriology |
description |
Escherichia albertii is a recently discovered species with a limited number of well characterized strains. The aim of this study was to characterize four of the E. albertii strains, which were among 41 identified Escherichia strains isolated from the feces of living animals on James Ross Island, Antarctica, and Isla Magdalena, Patagonia. Sequencing of 16S rDNA, automated ribotyping, and rep-PCR were used to identify the four E. albertii isolates. Phylogenetic analyses based on multi-locus sequence typing showed these isolates to be genetically most similar to the members of E. albertii phylogroup G3. These isolates encoded several virulence factors including those, which are characteristic of E. albertii (cytolethal distending toxin and intimin) as well as bacteriocin determinants that typically have a very low prevalence in E. coli strains (D, E7). Moreover, E. albertii protein extracts caused cell cycle arrest in human cell line A375, probably because of cytolethal distending toxin activity. |
format |
Text |
author |
GRILLOVÁ, Linda SEDLÁČEK, Ivo PÁCHNÍKOVÁ, Gabriela STAŇKOVÁ, Eva ŠVEC, Pavel HOLOCHOVÁ, Pavla MICENKOVÁ, Lenka BOSÁK, Juraj SLANINOVÁ, Iva ŠMAJS, David |
author_facet |
GRILLOVÁ, Linda SEDLÁČEK, Ivo PÁCHNÍKOVÁ, Gabriela STAŇKOVÁ, Eva ŠVEC, Pavel HOLOCHOVÁ, Pavla MICENKOVÁ, Lenka BOSÁK, Juraj SLANINOVÁ, Iva ŠMAJS, David |
author_sort |
GRILLOVÁ, Linda |
title |
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia |
title_short |
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia |
title_full |
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia |
title_fullStr |
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia |
title_full_unstemmed |
Characterization of four Escherichia albertii isolates collected from animals living in Antarctica and Patagonia |
title_sort |
characterization of four escherichia albertii isolates collected from animals living in antarctica and patagonia |
publisher |
The Japanese Society of Veterinary Science |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797873/ http://www.ncbi.nlm.nih.gov/pubmed/29249728 https://doi.org/10.1292/jvms.17-0492 |
geographic |
Patagonia Ross Island |
geographic_facet |
Patagonia Ross Island |
genre |
Antarc* Antarctica James Ross Island Ross Island |
genre_facet |
Antarc* Antarctica James Ross Island Ross Island |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5797873/ http://www.ncbi.nlm.nih.gov/pubmed/29249728 http://dx.doi.org/10.1292/jvms.17-0492 |
op_rights |
©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
op_rightsnorm |
CC-BY-NC-ND |
op_doi |
https://doi.org/10.1292/jvms.17-0492 |
container_title |
Journal of Veterinary Medical Science |
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80 |
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1 |
container_start_page |
138 |
op_container_end_page |
146 |
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1766269668467671040 |