Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria
Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) w...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795064/ http://www.ncbi.nlm.nih.gov/pubmed/29180360 https://doi.org/10.1128/AEM.01624-17 |
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ftpubmed:oai:pubmedcentral.nih.gov:5795064 2023-05-15T14:07:12+02:00 Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Finore, Ilaria Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina 2018-01-31 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795064/ http://www.ncbi.nlm.nih.gov/pubmed/29180360 https://doi.org/10.1128/AEM.01624-17 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795064/ http://www.ncbi.nlm.nih.gov/pubmed/29180360 http://dx.doi.org/10.1128/AEM.01624-17 Copyright © 2018 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2 All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) . Biotechnology Text 2018 ftpubmed https://doi.org/10.1128/AEM.01624-17 2018-08-05T00:20:44Z Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) was enhanced by a step-by-step approach, varying the carbon source, substrate and NaCl concentrations, temperature, and pH. The EPSs produced under optimal conditions were chemically characterized, resulting in a moderate carbohydrate content (range, 15 to 28%) and the presence of proteins (range, 3 to 24%) and uronic acids (range, 3.2 to 11.9%). Chemical hydrolysis of the carbohydrate portion revealed galactose, glucose, galactosamine, and mannose as the principal constituents. The potential biotechnological applications of the EPSs were also investigated. The high protein content in the EPSs from Winogradskyella sp. CAL384 was probably responsible for the excellent emulsifying activity toward tested hydrocarbons, with a stable emulsification index (E24) higher than those recorded for synthetic surfactants. All the EPSs tested in this work improved the freeze-thaw survival ratio of the isolates, suggesting that they may be exploited as cryoprotection agents. The addition of a sugar in the culture medium, by stimulating EPS production, also allowed isolates to grow in the presence of higher concentrations of mercury and cadmium. This finding was probably dependent on the presence of uronic acids and sulfate groups, which can act as ligands for cations, in the extracted EPSs. Text Antarc* Antarctic PubMed Central (PMC) Antarctic Applied and Environmental Microbiology 84 4 |
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English |
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Biotechnology |
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Biotechnology Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Finore, Ilaria Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria |
topic_facet |
Biotechnology |
description |
Four sponge-associated Antarctic bacteria (i.e., Winogradskyella sp. strains CAL384 and CAL396, Colwellia sp. strain GW185, and Shewanella sp. strain CAL606) were selected for the highly mucous appearance of their colonies on agar plates. The production of extracellular polymeric substances (EPSs) was enhanced by a step-by-step approach, varying the carbon source, substrate and NaCl concentrations, temperature, and pH. The EPSs produced under optimal conditions were chemically characterized, resulting in a moderate carbohydrate content (range, 15 to 28%) and the presence of proteins (range, 3 to 24%) and uronic acids (range, 3.2 to 11.9%). Chemical hydrolysis of the carbohydrate portion revealed galactose, glucose, galactosamine, and mannose as the principal constituents. The potential biotechnological applications of the EPSs were also investigated. The high protein content in the EPSs from Winogradskyella sp. CAL384 was probably responsible for the excellent emulsifying activity toward tested hydrocarbons, with a stable emulsification index (E24) higher than those recorded for synthetic surfactants. All the EPSs tested in this work improved the freeze-thaw survival ratio of the isolates, suggesting that they may be exploited as cryoprotection agents. The addition of a sugar in the culture medium, by stimulating EPS production, also allowed isolates to grow in the presence of higher concentrations of mercury and cadmium. This finding was probably dependent on the presence of uronic acids and sulfate groups, which can act as ligands for cations, in the extracted EPSs. |
format |
Text |
author |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Finore, Ilaria Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
author_facet |
Caruso, Consolazione Rizzo, Carmen Mangano, Santina Poli, Annarita Di Donato, Paola Finore, Ilaria Nicolaus, Barbara Di Marco, Gaetano Michaud, Luigi Lo Giudice, Angelina |
author_sort |
Caruso, Consolazione |
title |
Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria |
title_short |
Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria |
title_full |
Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria |
title_fullStr |
Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria |
title_full_unstemmed |
Production and Biotechnological Potential of Extracellular Polymeric Substances from Sponge-Associated Antarctic Bacteria |
title_sort |
production and biotechnological potential of extracellular polymeric substances from sponge-associated antarctic bacteria |
publisher |
American Society for Microbiology |
publishDate |
2018 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795064/ http://www.ncbi.nlm.nih.gov/pubmed/29180360 https://doi.org/10.1128/AEM.01624-17 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5795064/ http://www.ncbi.nlm.nih.gov/pubmed/29180360 http://dx.doi.org/10.1128/AEM.01624-17 |
op_rights |
Copyright © 2018 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2 All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) . |
op_doi |
https://doi.org/10.1128/AEM.01624-17 |
container_title |
Applied and Environmental Microbiology |
container_volume |
84 |
container_issue |
4 |
_version_ |
1766279101701685248 |