Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals
Host diet influences the diversity and metabolic activities of the gut microbiome. Previous studies have shown that the gut microbiome provides a wide array of enzymes that enable processing of diverse dietary components. Because the primary diet of the porcupine, Erethizon dorsatum, is lignified pl...
Published in: | PLOS ONE |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Public Library of Science
2017
|
Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744928/ http://www.ncbi.nlm.nih.gov/pubmed/29281673 https://doi.org/10.1371/journal.pone.0189404 |
id |
ftpubmed:oai:pubmedcentral.nih.gov:5744928 |
---|---|
record_format |
openpolar |
spelling |
ftpubmed:oai:pubmedcentral.nih.gov:5744928 2023-05-15T15:08:38+02:00 Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals Finlayson-Trick, Emma C. L. Getz, Landon J. Slaine, Patrick D. Thornbury, Mackenzie Lamoureux, Emily Cook, Jamie Langille, Morgan G. I. Murray, Lois E. McCormick, Craig Rohde, John R. Cheng, Zhenyu 2017-12-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744928/ http://www.ncbi.nlm.nih.gov/pubmed/29281673 https://doi.org/10.1371/journal.pone.0189404 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744928/ http://www.ncbi.nlm.nih.gov/pubmed/29281673 http://dx.doi.org/10.1371/journal.pone.0189404 © 2017 Finlayson-Trick et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2017 ftpubmed https://doi.org/10.1371/journal.pone.0189404 2018-01-14T01:11:37Z Host diet influences the diversity and metabolic activities of the gut microbiome. Previous studies have shown that the gut microbiome provides a wide array of enzymes that enable processing of diverse dietary components. Because the primary diet of the porcupine, Erethizon dorsatum, is lignified plant material, we reasoned that the porcupine microbiome would be replete with enzymes required to degrade lignocellulose. Here, we report on the bacterial composition in the porcupine microbiome using 16S rRNA sequencing and bioinformatics analysis. We extended this analysis to the microbiomes of 20 additional mammals located in Shubenacadie Wildlife Park (Nova Scotia, Canada), enabling the comparison of bacterial diversity amongst three mammalian taxonomic orders (Rodentia, Carnivora, and Artiodactyla). 16S rRNA sequencing was validated using metagenomic shotgun sequencing on selected herbivores (porcupine, beaver) and carnivores (coyote, Arctic wolf). In the microbiome, functionality is more conserved than bacterial composition, thus we mined microbiome data sets to identify conserved microbial functions across species in each order. We measured the relative gene abundances for cellobiose phosphorylase, endoglucanase, and beta-glucosidase to evaluate the cellulose-degrading potential of select mammals. The porcupine and beaver had higher proportions of genes encoding cellulose-degrading enzymes than the Artic wolf and coyote. These findings provide further evidence that gut microbiome diversity and metabolic capacity are influenced by host diet. Text Arctic PubMed Central (PMC) Arctic Canada PLOS ONE 12 12 e0189404 |
institution |
Open Polar |
collection |
PubMed Central (PMC) |
op_collection_id |
ftpubmed |
language |
English |
topic |
Research Article |
spellingShingle |
Research Article Finlayson-Trick, Emma C. L. Getz, Landon J. Slaine, Patrick D. Thornbury, Mackenzie Lamoureux, Emily Cook, Jamie Langille, Morgan G. I. Murray, Lois E. McCormick, Craig Rohde, John R. Cheng, Zhenyu Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals |
topic_facet |
Research Article |
description |
Host diet influences the diversity and metabolic activities of the gut microbiome. Previous studies have shown that the gut microbiome provides a wide array of enzymes that enable processing of diverse dietary components. Because the primary diet of the porcupine, Erethizon dorsatum, is lignified plant material, we reasoned that the porcupine microbiome would be replete with enzymes required to degrade lignocellulose. Here, we report on the bacterial composition in the porcupine microbiome using 16S rRNA sequencing and bioinformatics analysis. We extended this analysis to the microbiomes of 20 additional mammals located in Shubenacadie Wildlife Park (Nova Scotia, Canada), enabling the comparison of bacterial diversity amongst three mammalian taxonomic orders (Rodentia, Carnivora, and Artiodactyla). 16S rRNA sequencing was validated using metagenomic shotgun sequencing on selected herbivores (porcupine, beaver) and carnivores (coyote, Arctic wolf). In the microbiome, functionality is more conserved than bacterial composition, thus we mined microbiome data sets to identify conserved microbial functions across species in each order. We measured the relative gene abundances for cellobiose phosphorylase, endoglucanase, and beta-glucosidase to evaluate the cellulose-degrading potential of select mammals. The porcupine and beaver had higher proportions of genes encoding cellulose-degrading enzymes than the Artic wolf and coyote. These findings provide further evidence that gut microbiome diversity and metabolic capacity are influenced by host diet. |
format |
Text |
author |
Finlayson-Trick, Emma C. L. Getz, Landon J. Slaine, Patrick D. Thornbury, Mackenzie Lamoureux, Emily Cook, Jamie Langille, Morgan G. I. Murray, Lois E. McCormick, Craig Rohde, John R. Cheng, Zhenyu |
author_facet |
Finlayson-Trick, Emma C. L. Getz, Landon J. Slaine, Patrick D. Thornbury, Mackenzie Lamoureux, Emily Cook, Jamie Langille, Morgan G. I. Murray, Lois E. McCormick, Craig Rohde, John R. Cheng, Zhenyu |
author_sort |
Finlayson-Trick, Emma C. L. |
title |
Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals |
title_short |
Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals |
title_full |
Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals |
title_fullStr |
Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals |
title_full_unstemmed |
Taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals |
title_sort |
taxonomic differences of gut microbiomes drive cellulolytic enzymatic potential within hind-gut fermenting mammals |
publisher |
Public Library of Science |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744928/ http://www.ncbi.nlm.nih.gov/pubmed/29281673 https://doi.org/10.1371/journal.pone.0189404 |
geographic |
Arctic Canada |
geographic_facet |
Arctic Canada |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5744928/ http://www.ncbi.nlm.nih.gov/pubmed/29281673 http://dx.doi.org/10.1371/journal.pone.0189404 |
op_rights |
© 2017 Finlayson-Trick et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1371/journal.pone.0189404 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
12 |
container_start_page |
e0189404 |
_version_ |
1766339952936747008 |