Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches
Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) were analyzed using cytological and molecular approaches. In all three populations studied from Finland, i.e. Turku, Kustavi and Siikajoki, males were present at a low frequency but were absent from a population from Vorkuta, Russia....
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ftpubmed:oai:pubmedcentral.nih.gov:5740394 2023-05-15T18:42:55+02:00 Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches Nokkala, Seppo Kuznetsova, Valentina G. Nokkala, Christina 2017-11-24 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740394/ https://doi.org/10.3897/CompCytogen.v11i4.21362 en eng Pensoft Publishers http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740394/ http://dx.doi.org/10.3897/CompCytogen.v11i4.21362 Seppo Nokkala, Valentina G. Kuznetsova, Christina Nokkala http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2017 ftpubmed https://doi.org/10.3897/CompCytogen.v11i4.21362 2018-01-07T01:27:10Z Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) were analyzed using cytological and molecular approaches. In all three populations studied from Finland, i.e. Turku, Kustavi and Siikajoki, males were present at a low frequency but were absent from a population from Vorkuta, Russia. In a follow-up study conducted in the Turku population during 2010–2016, the initial frequency of males was ca. 10 % and showed no intraseasonal variation, but then dramatically decreased down to approximately 1–2 % level in seasons 2015–2016. Male meiosis was chiasmate with some traces of chromosomal fragmentation and subsequent fusions. In most females, metaphase in mature eggs included 39 univalent chromosomes which indicated apomictic triploidy. Only a small fraction of females was diploid with 13 chiasmate bivalents. The frequency of diploid females approximately equaled that of males. COI barcode analyses showed that triploid females (N = 57) and diploids (7 females and 5 males) displayed different haplotypes, demonstrating that triploid females reproduced via obligate parthenogenesis. The rarity of diploids, along with the lack of males’ preference towards diploid females, suggested that most likely diploids were produced by rare triploid females which shared the same haplotype with the diploids (not found in the present analysis). Minimum haplotype diversity was detected in the Turku population, but it was much higher in Vorkuta with some indication for the mixed origin of the population. We suggest that functional diploids produced in a parthenogenetic population can give rise either to a new parthenogenetic lineage or even to a new bisexual species. Text Vorkuta PubMed Central (PMC) Comparative Cytogenetics 11 4 807 817 |
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Research Article Nokkala, Seppo Kuznetsova, Valentina G. Nokkala, Christina Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches |
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Research Article |
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Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) were analyzed using cytological and molecular approaches. In all three populations studied from Finland, i.e. Turku, Kustavi and Siikajoki, males were present at a low frequency but were absent from a population from Vorkuta, Russia. In a follow-up study conducted in the Turku population during 2010–2016, the initial frequency of males was ca. 10 % and showed no intraseasonal variation, but then dramatically decreased down to approximately 1–2 % level in seasons 2015–2016. Male meiosis was chiasmate with some traces of chromosomal fragmentation and subsequent fusions. In most females, metaphase in mature eggs included 39 univalent chromosomes which indicated apomictic triploidy. Only a small fraction of females was diploid with 13 chiasmate bivalents. The frequency of diploid females approximately equaled that of males. COI barcode analyses showed that triploid females (N = 57) and diploids (7 females and 5 males) displayed different haplotypes, demonstrating that triploid females reproduced via obligate parthenogenesis. The rarity of diploids, along with the lack of males’ preference towards diploid females, suggested that most likely diploids were produced by rare triploid females which shared the same haplotype with the diploids (not found in the present analysis). Minimum haplotype diversity was detected in the Turku population, but it was much higher in Vorkuta with some indication for the mixed origin of the population. We suggest that functional diploids produced in a parthenogenetic population can give rise either to a new parthenogenetic lineage or even to a new bisexual species. |
format |
Text |
author |
Nokkala, Seppo Kuznetsova, Valentina G. Nokkala, Christina |
author_facet |
Nokkala, Seppo Kuznetsova, Valentina G. Nokkala, Christina |
author_sort |
Nokkala, Seppo |
title |
Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches |
title_short |
Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches |
title_full |
Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches |
title_fullStr |
Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches |
title_full_unstemmed |
Characteristics of parthenogenesis in Cacopsylla ledi (Flor, 1861) (Hemiptera, Sternorryncha, Psylloidea): cytological and molecular approaches |
title_sort |
characteristics of parthenogenesis in cacopsylla ledi (flor, 1861) (hemiptera, sternorryncha, psylloidea): cytological and molecular approaches |
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Pensoft Publishers |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740394/ https://doi.org/10.3897/CompCytogen.v11i4.21362 |
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Vorkuta |
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Vorkuta |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5740394/ http://dx.doi.org/10.3897/CompCytogen.v11i4.21362 |
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Seppo Nokkala, Valentina G. Kuznetsova, Christina Nokkala http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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https://doi.org/10.3897/CompCytogen.v11i4.21362 |
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