Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba
Antarctic krill (Euphausia superba) is a key species in Southern Ocean ecosystem where it plays a central role in the Antarctic food web. Available information supports the existence of an endogenous timing system in krill enabling it to synchronize metabolism and behavior with an environment charac...
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ftpubmed:oai:pubmedcentral.nih.gov:5735174 2023-05-15T13:41:05+02:00 Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà, Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo 2017-12-18 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735174/ http://www.ncbi.nlm.nih.gov/pubmed/29255161 https://doi.org/10.1038/s41598-017-18009-2 en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735174/ http://www.ncbi.nlm.nih.gov/pubmed/29255161 http://dx.doi.org/10.1038/s41598-017-18009-2 © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2017 ftpubmed https://doi.org/10.1038/s41598-017-18009-2 2017-12-24T01:23:00Z Antarctic krill (Euphausia superba) is a key species in Southern Ocean ecosystem where it plays a central role in the Antarctic food web. Available information supports the existence of an endogenous timing system in krill enabling it to synchronize metabolism and behavior with an environment characterized by extreme seasonal changes in terms of day length, food availability, and surface ice extent. A screening of our transcriptome database “KrillDB” allowed us to identify the putative orthologues of 20 circadian clock components. Mapping of conserved domains and phylogenetic analyses strongly supported annotations of the identified sequences. Luciferase assays and co-immunoprecipitation experiments allowed us to define the role of the main clock components. Our findings provide an overall picture of the molecular mechanisms underlying the functioning of the endogenous circadian clock in the Antarctic krill and shed light on their evolution throughout crustaceans speciation. Interestingly, the core clock machinery shows both mammalian and insect features that presumably contribute to an evolutionary strategy to cope with polar environment’s challenges. Moreover, despite the extreme variability characterizing the Antarctic seasonal day length, the conserved light mediated degradation of the photoreceptor EsCRY1 suggests a persisting pivotal role of light as a Zeitgeber. Text Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean PubMed Central (PMC) Antarctic Southern Ocean The Antarctic Scientific Reports 7 1 |
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Article Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà, Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
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Article |
description |
Antarctic krill (Euphausia superba) is a key species in Southern Ocean ecosystem where it plays a central role in the Antarctic food web. Available information supports the existence of an endogenous timing system in krill enabling it to synchronize metabolism and behavior with an environment characterized by extreme seasonal changes in terms of day length, food availability, and surface ice extent. A screening of our transcriptome database “KrillDB” allowed us to identify the putative orthologues of 20 circadian clock components. Mapping of conserved domains and phylogenetic analyses strongly supported annotations of the identified sequences. Luciferase assays and co-immunoprecipitation experiments allowed us to define the role of the main clock components. Our findings provide an overall picture of the molecular mechanisms underlying the functioning of the endogenous circadian clock in the Antarctic krill and shed light on their evolution throughout crustaceans speciation. Interestingly, the core clock machinery shows both mammalian and insect features that presumably contribute to an evolutionary strategy to cope with polar environment’s challenges. Moreover, despite the extreme variability characterizing the Antarctic seasonal day length, the conserved light mediated degradation of the photoreceptor EsCRY1 suggests a persisting pivotal role of light as a Zeitgeber. |
format |
Text |
author |
Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà, Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo |
author_facet |
Biscontin, Alberto Wallach, Thomas Sales, Gabriele Grudziecki, Astrid Janke, Leonard Sartori, Elena Bertolucci, Cristiano Mazzotta, Gabriella De Pittà, Cristiano Meyer, Bettina Kramer, Achim Costa, Rodolfo |
author_sort |
Biscontin, Alberto |
title |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_short |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_full |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_fullStr |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_full_unstemmed |
Functional characterization of the circadian clock in the Antarctic krill, Euphausia superba |
title_sort |
functional characterization of the circadian clock in the antarctic krill, euphausia superba |
publisher |
Nature Publishing Group UK |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735174/ http://www.ncbi.nlm.nih.gov/pubmed/29255161 https://doi.org/10.1038/s41598-017-18009-2 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5735174/ http://www.ncbi.nlm.nih.gov/pubmed/29255161 http://dx.doi.org/10.1038/s41598-017-18009-2 |
op_rights |
© The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-017-18009-2 |
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Scientific Reports |
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7 |
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1 |
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1766145581997096960 |