Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats
In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. Metagenomic technologies are powerful tools that can give a more comprehensive assessment of microbial communities, and th...
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ftpubmed:oai:pubmedcentral.nih.gov:5694453 2023-05-15T14:05:10+02:00 Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats Baeza, Marcelo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor 2017-11-14 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694453/ https://doi.org/10.3389/fmicb.2017.02235 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694453/ http://dx.doi.org/10.3389/fmicb.2017.02235 Copyright © 2017 Baeza, Barahona, Alcaíno and Cifuentes. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2017 ftpubmed https://doi.org/10.3389/fmicb.2017.02235 2017-12-03T01:28:15Z In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. Metagenomic technologies are powerful tools that can give a more comprehensive assessment of microbial communities, and the amplification of rDNA followed by next-generation sequencing has given good results in studies aimed particularly at environmental microorganisms. Culture-independent studies of microbiota in terrestrial habitats of Antarctica, which is considered the driest, coldest climate on Earth, are increasing and indicate that micro-diversity is much higher than previously thought. In this work, the microbial diversity of terrestrial habitats including eight islands of the South Shetland Archipelago, two islands on the Antarctic Peninsula and Union Glacier, was studied by amplicon-metagenome analysis. Molecular analysis of the studied localities clustered together the islands of the South Shetland Archipelago, except Greenwich Island, and separated them from the Litchfield and Lagotellerie islands and Union Glacier, which is in agreement with the latitudinal difference among them. Among fungi, 87 genera and 123 species were found, of which species belonging to 37 fungal genera not previously cultivated from Antarctica were detected. Phylogenetic analysis, including the closest BLAST-hit sequences, clustered fungi in 11 classes being the most represented Lecanoromycetes and Eurotiomycetes. Text Antarc* Antarctic Antarctic Peninsula Antarctica Greenwich Island Union Glacier PubMed Central (PMC) Antarctic Antarctic Peninsula Greenwich Greenwich Island ENVELOPE(-59.783,-59.783,-62.517,-62.517) Lagotellerie ENVELOPE(-67.402,-67.402,-67.886,-67.886) Litchfield ENVELOPE(-64.100,-64.100,-64.783,-64.783) The Antarctic Union Glacier ENVELOPE(-82.500,-82.500,-79.750,-79.750) Frontiers in Microbiology 8 |
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English |
topic |
Microbiology |
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Microbiology Baeza, Marcelo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
topic_facet |
Microbiology |
description |
In cold environments such as polar regions, microorganisms play important ecological roles, and most of our knowledge about them comes from studies of cultivable microorganisms. Metagenomic technologies are powerful tools that can give a more comprehensive assessment of microbial communities, and the amplification of rDNA followed by next-generation sequencing has given good results in studies aimed particularly at environmental microorganisms. Culture-independent studies of microbiota in terrestrial habitats of Antarctica, which is considered the driest, coldest climate on Earth, are increasing and indicate that micro-diversity is much higher than previously thought. In this work, the microbial diversity of terrestrial habitats including eight islands of the South Shetland Archipelago, two islands on the Antarctic Peninsula and Union Glacier, was studied by amplicon-metagenome analysis. Molecular analysis of the studied localities clustered together the islands of the South Shetland Archipelago, except Greenwich Island, and separated them from the Litchfield and Lagotellerie islands and Union Glacier, which is in agreement with the latitudinal difference among them. Among fungi, 87 genera and 123 species were found, of which species belonging to 37 fungal genera not previously cultivated from Antarctica were detected. Phylogenetic analysis, including the closest BLAST-hit sequences, clustered fungi in 11 classes being the most represented Lecanoromycetes and Eurotiomycetes. |
format |
Text |
author |
Baeza, Marcelo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor |
author_facet |
Baeza, Marcelo Barahona, Salvador Alcaíno, Jennifer Cifuentes, Víctor |
author_sort |
Baeza, Marcelo |
title |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_short |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_full |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_fullStr |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_full_unstemmed |
Amplicon-Metagenomic Analysis of Fungi from Antarctic Terrestrial Habitats |
title_sort |
amplicon-metagenomic analysis of fungi from antarctic terrestrial habitats |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694453/ https://doi.org/10.3389/fmicb.2017.02235 |
long_lat |
ENVELOPE(-59.783,-59.783,-62.517,-62.517) ENVELOPE(-67.402,-67.402,-67.886,-67.886) ENVELOPE(-64.100,-64.100,-64.783,-64.783) ENVELOPE(-82.500,-82.500,-79.750,-79.750) |
geographic |
Antarctic Antarctic Peninsula Greenwich Greenwich Island Lagotellerie Litchfield The Antarctic Union Glacier |
geographic_facet |
Antarctic Antarctic Peninsula Greenwich Greenwich Island Lagotellerie Litchfield The Antarctic Union Glacier |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Greenwich Island Union Glacier |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Greenwich Island Union Glacier |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5694453/ http://dx.doi.org/10.3389/fmicb.2017.02235 |
op_rights |
Copyright © 2017 Baeza, Barahona, Alcaíno and Cifuentes. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2017.02235 |
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Frontiers in Microbiology |
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8 |
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1766276881927110656 |