Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids
Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limi...
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ftpubmed:oai:pubmedcentral.nih.gov:5660062 2023-05-15T18:06:08+02:00 Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids Liu, Jinxin Zhao, Zhe Deng, Yiqing Shi, Yan Liu, Yupeng Wu, Chao Luo, Peng Hu, Chaoqun 2017-10-23 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660062/ http://www.ncbi.nlm.nih.gov/pubmed/29109705 https://doi.org/10.3389/fmicb.2017.02035 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660062/ http://www.ncbi.nlm.nih.gov/pubmed/29109705 http://dx.doi.org/10.3389/fmicb.2017.02035 Copyright © 2017 Liu, Zhao, Deng, Shi, Liu, Wu, Luo and Hu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2017 ftpubmed https://doi.org/10.3389/fmicb.2017.02035 2017-11-12T01:11:08Z Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp. is currently lacking. In this study, the complete genome of a V. campbellii predominant strain, LMB29, was determined by MiSeq in conjunction with PacBio SMRT sequencing. This genome consists of two circular DNA chromosomes and four megaplasmids. Comparative genome analysis indicates that LMB29 shares a 96.66% similarity (average nucleotide identity) with the V. campbellii ATCC strain BAA-1116 based on a 75% AF (average fraction) calculations, and its functional profile is very similar to V. campbellii E1 and V. campbellii CAIM115. Both type III secretion system (T3SS) and type VI secretion system (T6SS), along with the tlh gene which encodes a thermolabile hemolysin, are present in LMB29 which may contribute to the bacterial pathogenesis. The virulence of this strain was experimental confirmed by performing a LDH assay on a fish cell infection model, and cell death was observed as early as within 3 h post infection. Thirty-seven antimicrobial resistance genes (>45% identity) were predicted in LMB29 which includes a novel rifampicin ADP ribosyltransferase, arr-9, in plasmid pLMB157. The gene arr-9 was predicted on a genomic island with horizontal transferable potentials which may facilitate the rifampicin resistance dissemination. Future researches are needed to explore the pathogenesis of V. campbellii LMB29, but the availability of this genome sequence will certainly aid as a basis for further analysis. Text Red drum PubMed Central (PMC) Frontiers in Microbiology 8 |
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Microbiology |
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Microbiology Liu, Jinxin Zhao, Zhe Deng, Yiqing Shi, Yan Liu, Yupeng Wu, Chao Luo, Peng Hu, Chaoqun Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
topic_facet |
Microbiology |
description |
Vibrio spp. are the most common pathogens for animals reared in aquaculture. Vibrio campbellii, which is often involved in shrimp, fish and mollusks diseases, is widely distributed in the marine environment worldwide, but our knowledge about its pathogenesis and antimicrobial resistance is very limited. The existence of this knowledge gap is at least partially because that V. campbellii was originally classified as Vibrio harveyi, and the detailed information of its comparative genome analysis to other Vibrio spp. is currently lacking. In this study, the complete genome of a V. campbellii predominant strain, LMB29, was determined by MiSeq in conjunction with PacBio SMRT sequencing. This genome consists of two circular DNA chromosomes and four megaplasmids. Comparative genome analysis indicates that LMB29 shares a 96.66% similarity (average nucleotide identity) with the V. campbellii ATCC strain BAA-1116 based on a 75% AF (average fraction) calculations, and its functional profile is very similar to V. campbellii E1 and V. campbellii CAIM115. Both type III secretion system (T3SS) and type VI secretion system (T6SS), along with the tlh gene which encodes a thermolabile hemolysin, are present in LMB29 which may contribute to the bacterial pathogenesis. The virulence of this strain was experimental confirmed by performing a LDH assay on a fish cell infection model, and cell death was observed as early as within 3 h post infection. Thirty-seven antimicrobial resistance genes (>45% identity) were predicted in LMB29 which includes a novel rifampicin ADP ribosyltransferase, arr-9, in plasmid pLMB157. The gene arr-9 was predicted on a genomic island with horizontal transferable potentials which may facilitate the rifampicin resistance dissemination. Future researches are needed to explore the pathogenesis of V. campbellii LMB29, but the availability of this genome sequence will certainly aid as a basis for further analysis. |
format |
Text |
author |
Liu, Jinxin Zhao, Zhe Deng, Yiqing Shi, Yan Liu, Yupeng Wu, Chao Luo, Peng Hu, Chaoqun |
author_facet |
Liu, Jinxin Zhao, Zhe Deng, Yiqing Shi, Yan Liu, Yupeng Wu, Chao Luo, Peng Hu, Chaoqun |
author_sort |
Liu, Jinxin |
title |
Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
title_short |
Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
title_full |
Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
title_fullStr |
Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
title_full_unstemmed |
Complete Genome Sequence of Vibrio campbellii LMB 29 Isolated from Red Drum with Four Native Megaplasmids |
title_sort |
complete genome sequence of vibrio campbellii lmb 29 isolated from red drum with four native megaplasmids |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660062/ http://www.ncbi.nlm.nih.gov/pubmed/29109705 https://doi.org/10.3389/fmicb.2017.02035 |
genre |
Red drum |
genre_facet |
Red drum |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660062/ http://www.ncbi.nlm.nih.gov/pubmed/29109705 http://dx.doi.org/10.3389/fmicb.2017.02035 |
op_rights |
Copyright © 2017 Liu, Zhao, Deng, Shi, Liu, Wu, Luo and Hu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2017.02035 |
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Frontiers in Microbiology |
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8 |
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1766177725972742144 |