Variability in the protein profiles in spermatozoa of two sturgeon species
Conventional sperm analysis (i.e., motility and fertility) has been used to evaluate sperm quality. Understanding the quality of sperm on the molecular level in the sturgeons, Acipenser baerii and A. schrenckii, is essential for the improvement of the conservation of genetic resources and farming pe...
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ftpubmed:oai:pubmedcentral.nih.gov:5659609 2023-05-15T13:01:53+02:00 Variability in the protein profiles in spermatozoa of two sturgeon species Li, Ping Guo, Wei Yue, Huamei Li, Chuangju Du, Hao Qiao, Xinmei Liu, Zhigang Zhou, Qiong Wei, Qiwei 2017-10-27 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659609/ http://www.ncbi.nlm.nih.gov/pubmed/29077704 https://doi.org/10.1371/journal.pone.0186003 en eng Public Library of Science http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659609/ http://www.ncbi.nlm.nih.gov/pubmed/29077704 http://dx.doi.org/10.1371/journal.pone.0186003 © 2017 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2017 ftpubmed https://doi.org/10.1371/journal.pone.0186003 2017-11-12T01:10:59Z Conventional sperm analysis (i.e., motility and fertility) has been used to evaluate sperm quality. Understanding the quality of sperm on the molecular level in the sturgeons, Acipenser baerii and A. schrenckii, is essential for the improvement of the conservation of genetic resources and farming performance. In this study, we used the iTRAQ proteomics approach to perform proteomic profiling of spermatozoa associated with sperm quality in sturgeons (Data are available via ProteomeXchange with identifier PXD006108). The results showed 291 and 359 differentially expressed proteins in A. baerii and A. schrenckii, respectively, of which 72 were common to both species and all were upregulated in high quality compared with low quality samples. The differentially expressed proteins were mainly categorized into the generation of precursor metabolites and energy and oxidation, and they were localized to the mitochondria. Three distinguishing pathways, Arginine and proline metabolism, Pyruvate metabolism and the Citrate cycle (TCA cycle) were found to play an important role in energy metabolism, and some substrates could be used in the sperm medium for storage and cryopreservation. The quantity levels of two proteins, CKMT1 and LDHB, were verified by western blot analysis. Moreover, other potential biomarkers involved in oxidation reduction, ubiquitin-proteasome-dependent proteolysis, chaperones and binding activity were also discussed. Our study is the first to use the iTRAQ-based proteomics approach to analyse the sturgeon spermatozoa proteome, and the results that we obtained are valuable for the prediction of sperm quality and reproduction management in these threatened species. Text Acipenser baerii PubMed Central (PMC) PLOS ONE 12 10 e0186003 |
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Research Article Li, Ping Guo, Wei Yue, Huamei Li, Chuangju Du, Hao Qiao, Xinmei Liu, Zhigang Zhou, Qiong Wei, Qiwei Variability in the protein profiles in spermatozoa of two sturgeon species |
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Research Article |
description |
Conventional sperm analysis (i.e., motility and fertility) has been used to evaluate sperm quality. Understanding the quality of sperm on the molecular level in the sturgeons, Acipenser baerii and A. schrenckii, is essential for the improvement of the conservation of genetic resources and farming performance. In this study, we used the iTRAQ proteomics approach to perform proteomic profiling of spermatozoa associated with sperm quality in sturgeons (Data are available via ProteomeXchange with identifier PXD006108). The results showed 291 and 359 differentially expressed proteins in A. baerii and A. schrenckii, respectively, of which 72 were common to both species and all were upregulated in high quality compared with low quality samples. The differentially expressed proteins were mainly categorized into the generation of precursor metabolites and energy and oxidation, and they were localized to the mitochondria. Three distinguishing pathways, Arginine and proline metabolism, Pyruvate metabolism and the Citrate cycle (TCA cycle) were found to play an important role in energy metabolism, and some substrates could be used in the sperm medium for storage and cryopreservation. The quantity levels of two proteins, CKMT1 and LDHB, were verified by western blot analysis. Moreover, other potential biomarkers involved in oxidation reduction, ubiquitin-proteasome-dependent proteolysis, chaperones and binding activity were also discussed. Our study is the first to use the iTRAQ-based proteomics approach to analyse the sturgeon spermatozoa proteome, and the results that we obtained are valuable for the prediction of sperm quality and reproduction management in these threatened species. |
format |
Text |
author |
Li, Ping Guo, Wei Yue, Huamei Li, Chuangju Du, Hao Qiao, Xinmei Liu, Zhigang Zhou, Qiong Wei, Qiwei |
author_facet |
Li, Ping Guo, Wei Yue, Huamei Li, Chuangju Du, Hao Qiao, Xinmei Liu, Zhigang Zhou, Qiong Wei, Qiwei |
author_sort |
Li, Ping |
title |
Variability in the protein profiles in spermatozoa of two sturgeon species |
title_short |
Variability in the protein profiles in spermatozoa of two sturgeon species |
title_full |
Variability in the protein profiles in spermatozoa of two sturgeon species |
title_fullStr |
Variability in the protein profiles in spermatozoa of two sturgeon species |
title_full_unstemmed |
Variability in the protein profiles in spermatozoa of two sturgeon species |
title_sort |
variability in the protein profiles in spermatozoa of two sturgeon species |
publisher |
Public Library of Science |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659609/ http://www.ncbi.nlm.nih.gov/pubmed/29077704 https://doi.org/10.1371/journal.pone.0186003 |
genre |
Acipenser baerii |
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Acipenser baerii |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5659609/ http://www.ncbi.nlm.nih.gov/pubmed/29077704 http://dx.doi.org/10.1371/journal.pone.0186003 |
op_rights |
© 2017 Li et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
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https://doi.org/10.1371/journal.pone.0186003 |
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PLOS ONE |
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12 |
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