Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus
We present the first complete genome sequence of Odocoileus hemionus deer adenovirus 1 (OdAdV-1). This virus can cause sporadic haemorrhagic disease in cervids, although epizootics with high mortality have occurred in California. OdAdV-1 has been placed in the genus Atadenovirus, based on partial he...
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ftpubmed:oai:pubmedcentral.nih.gov:5656758 2023-05-15T13:13:33+02:00 Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus Miller, Myrna M. Cornish, Todd E. Creekmore, Terry E. Fox, Karen Laegreid, Will McKenna, Jennifer Vasquez, Marce Woods, Leslie W. 2017-09 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656758/ http://www.ncbi.nlm.nih.gov/pubmed/28809152 https://doi.org/10.1099/jgv.0.000880 en eng Microbiology Society http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656758/ http://www.ncbi.nlm.nih.gov/pubmed/28809152 http://dx.doi.org/10.1099/jgv.0.000880 © 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. CC-BY Research Article Text 2017 ftpubmed https://doi.org/10.1099/jgv.0.000880 2017-11-19T01:07:38Z We present the first complete genome sequence of Odocoileus hemionus deer adenovirus 1 (OdAdV-1). This virus can cause sporadic haemorrhagic disease in cervids, although epizootics with high mortality have occurred in California. OdAdV-1 has been placed in the genus Atadenovirus, based on partial hexon, pVIII and fibre genes. Ten field isolates recovered from naturally infected mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginiana) and moose (Alces alces) from Wyoming, black-tailed deer (Odocoileus hemionus columbianus) from California, and Rocky Mountain elk (Cervus elaphus nelsoni) from Colorado and Washington state were sequenced. The genome lengths ranged from 30 620 to 30 699 bp, contained the predicted proteins and gene organization typical of members of genus Atadenovirus, and had a high percentage of A/T nucleotides (66.7 %). Phylogenic analysis found that the closest ancestry was with ruminant atadenoviruses, while a divergence of the hexon, polymerase and penton base proteins of more than 15 % supports classification as a new species. Genetic global comparison between the 10 isolates found an overall 99 % identity, but greater divergence was found between those recovered from moose and elk as compared to deer, and a single variable region contained most of these differences. Our findings demonstrate that OdAdV-1 is highly conserved between 10 isolates recovered from multiple related cervid species, but genotypic differences, largely localized to a variable region, define two strains. We propose that the virus type name be changed to cervid adenovirus 1, with the species name Cervid atadenovirus A. Sequence data were used to develop molecular assays for improved detection and genotyping. Text Alces alces PubMed Central (PMC) Journal of General Virology 98 9 2320 2328 |
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Research Article Miller, Myrna M. Cornish, Todd E. Creekmore, Terry E. Fox, Karen Laegreid, Will McKenna, Jennifer Vasquez, Marce Woods, Leslie W. Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus |
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Research Article |
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We present the first complete genome sequence of Odocoileus hemionus deer adenovirus 1 (OdAdV-1). This virus can cause sporadic haemorrhagic disease in cervids, although epizootics with high mortality have occurred in California. OdAdV-1 has been placed in the genus Atadenovirus, based on partial hexon, pVIII and fibre genes. Ten field isolates recovered from naturally infected mule deer (Odocoileus hemionus), white-tailed deer (Odocoileus virginiana) and moose (Alces alces) from Wyoming, black-tailed deer (Odocoileus hemionus columbianus) from California, and Rocky Mountain elk (Cervus elaphus nelsoni) from Colorado and Washington state were sequenced. The genome lengths ranged from 30 620 to 30 699 bp, contained the predicted proteins and gene organization typical of members of genus Atadenovirus, and had a high percentage of A/T nucleotides (66.7 %). Phylogenic analysis found that the closest ancestry was with ruminant atadenoviruses, while a divergence of the hexon, polymerase and penton base proteins of more than 15 % supports classification as a new species. Genetic global comparison between the 10 isolates found an overall 99 % identity, but greater divergence was found between those recovered from moose and elk as compared to deer, and a single variable region contained most of these differences. Our findings demonstrate that OdAdV-1 is highly conserved between 10 isolates recovered from multiple related cervid species, but genotypic differences, largely localized to a variable region, define two strains. We propose that the virus type name be changed to cervid adenovirus 1, with the species name Cervid atadenovirus A. Sequence data were used to develop molecular assays for improved detection and genotyping. |
format |
Text |
author |
Miller, Myrna M. Cornish, Todd E. Creekmore, Terry E. Fox, Karen Laegreid, Will McKenna, Jennifer Vasquez, Marce Woods, Leslie W. |
author_facet |
Miller, Myrna M. Cornish, Todd E. Creekmore, Terry E. Fox, Karen Laegreid, Will McKenna, Jennifer Vasquez, Marce Woods, Leslie W. |
author_sort |
Miller, Myrna M. |
title |
Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus |
title_short |
Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus |
title_full |
Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus |
title_fullStr |
Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus |
title_full_unstemmed |
Whole-genome sequences of Odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus Atadenovirus |
title_sort |
whole-genome sequences of odocoileus hemionus deer adenovirus isolates from deer, moose and elk are highly conserved and support a new species in the genus atadenovirus |
publisher |
Microbiology Society |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656758/ http://www.ncbi.nlm.nih.gov/pubmed/28809152 https://doi.org/10.1099/jgv.0.000880 |
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Alces alces |
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Alces alces |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656758/ http://www.ncbi.nlm.nih.gov/pubmed/28809152 http://dx.doi.org/10.1099/jgv.0.000880 |
op_rights |
© 2017 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited. |
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CC-BY |
op_doi |
https://doi.org/10.1099/jgv.0.000880 |
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Journal of General Virology |
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98 |
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9 |
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2320 |
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2328 |
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1766259031724261376 |