Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating micr...
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ftpubmed:oai:pubmedcentral.nih.gov:5630931 2023-05-15T13:55:55+02:00 Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. 2016-08-08 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630931/ http://www.ncbi.nlm.nih.gov/pubmed/27503299 https://doi.org/10.1093/gbe/evw189 en eng Oxford University Press http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630931/ http://www.ncbi.nlm.nih.gov/pubmed/27503299 http://dx.doi.org/10.1093/gbe/evw189 © The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com CC-BY-NC Research Article Text 2016 ftpubmed https://doi.org/10.1093/gbe/evw189 2017-11-05T01:21:53Z Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the two metagenomes were identified. The Antarctic hypolithic metagenome displayed a high number of sequences assigned to sigma factors, replication, recombination and repair, translation, ribosomal structure, and biogenesis. In contrast, the Namib Desert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significant divergence in the genetic determinants of amino acid and nucleotide metabolism between these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Miers ENVELOPE(163.850,163.850,-78.100,-78.100) Miers Valley ENVELOPE(164.200,164.200,-78.100,-78.100) The Antarctic Genome Biology and Evolution 8 9 2737 2747 |
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Research Article Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts |
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description |
Understanding microbial adaptation to environmental stressors is crucial for interpreting broader ecological patterns. In the most extreme hot and cold deserts, cryptic niche communities are thought to play key roles in ecosystem processes and represent excellent model systems for investigating microbial responses to environmental stressors. However, relatively little is known about the genetic diversity underlying such functional processes in climatically extreme desert systems. This study presents the first comparative metagenome analysis of cyanobacteria-dominated hypolithic communities in hot (Namib Desert, Namibia) and cold (Miers Valley, Antarctica) hyperarid deserts. The most abundant phyla in both hypolith metagenomes were Actinobacteria, Proteobacteria, Cyanobacteria and Bacteroidetes with Cyanobacteria dominating in Antarctic hypoliths. However, no significant differences between the two metagenomes were identified. The Antarctic hypolithic metagenome displayed a high number of sequences assigned to sigma factors, replication, recombination and repair, translation, ribosomal structure, and biogenesis. In contrast, the Namib Desert metagenome showed a high abundance of sequences assigned to carbohydrate transport and metabolism. Metagenome data analysis also revealed significant divergence in the genetic determinants of amino acid and nucleotide metabolism between these two metagenomes and those of soil from other polar deserts, hot deserts, and non-desert soils. Our results suggest extensive niche differentiation in hypolithic microbial communities from these two extreme environments and a high genetic capacity for survival under environmental extremes. |
format |
Text |
author |
Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. |
author_facet |
Le, Phuong Thi Makhalanyane, Thulani P. Guerrero, Leandro D. Vikram, Surendra Van de Peer, Yves Cowan, Don A. |
author_sort |
Le, Phuong Thi |
title |
Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts |
title_short |
Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts |
title_full |
Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts |
title_fullStr |
Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts |
title_full_unstemmed |
Comparative Metagenomic Analysis Reveals Mechanisms for Stress Response in Hypoliths from Extreme Hyperarid Deserts |
title_sort |
comparative metagenomic analysis reveals mechanisms for stress response in hypoliths from extreme hyperarid deserts |
publisher |
Oxford University Press |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630931/ http://www.ncbi.nlm.nih.gov/pubmed/27503299 https://doi.org/10.1093/gbe/evw189 |
long_lat |
ENVELOPE(163.850,163.850,-78.100,-78.100) ENVELOPE(164.200,164.200,-78.100,-78.100) |
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Antarctic Miers Miers Valley The Antarctic |
geographic_facet |
Antarctic Miers Miers Valley The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5630931/ http://www.ncbi.nlm.nih.gov/pubmed/27503299 http://dx.doi.org/10.1093/gbe/evw189 |
op_rights |
© The Author(s) 2016. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
op_rightsnorm |
CC-BY-NC |
op_doi |
https://doi.org/10.1093/gbe/evw189 |
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Genome Biology and Evolution |
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8 |
container_issue |
9 |
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2737 |
op_container_end_page |
2747 |
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1766262893402128384 |