Sex steroid production associated with puberty is absent in germ cell-free salmon
In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To explore if the absence of germ cells affects puberty or growth in Atlantic salmon, germ ce...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626747/ http://www.ncbi.nlm.nih.gov/pubmed/28974703 https://doi.org/10.1038/s41598-017-12936-w |
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ftpubmed:oai:pubmedcentral.nih.gov:5626747 2023-05-15T15:32:29+02:00 Sex steroid production associated with puberty is absent in germ cell-free salmon Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B. Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W. Wargelius, Anna 2017-10-03 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626747/ http://www.ncbi.nlm.nih.gov/pubmed/28974703 https://doi.org/10.1038/s41598-017-12936-w en eng Nature Publishing Group UK http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626747/ http://www.ncbi.nlm.nih.gov/pubmed/28974703 http://dx.doi.org/10.1038/s41598-017-12936-w © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. CC-BY Article Text 2017 ftpubmed https://doi.org/10.1038/s41598-017-12936-w 2017-10-15T00:12:15Z In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To explore if the absence of germ cells affects puberty or growth in Atlantic salmon, germ cell-free (GCF), dnd knockout and wild type (WT) postsmolts were stimulated to enter puberty. No GCF fish entered puberty, whereas 66.7% (males) and 30% (females) WT fish completed or entered puberty, respectively. Expression of genes related to steroidogenesis (star, cyp17a1, cyp11β, cyp19a1a), gonadal somatic cells (insl3, amh, igf3), oocytes (bmp15), gonadotropin receptors (fshr, lhcgr), and pituitary gonadotropic cells (fshb, lhb, gnrhr4) showed an immature status and failure to up-regulate gonadal sex steroid production in male and female GCF fish was also reflected in low or undetectable plasma sex steroids (11-ketotestosterone, estradiol-17β and testosterone). A gender difference (high in females, low in males) was found in the expression of star and cyp17a1 in GCF fish. No clear difference in growth was detected between GCF and immature WT fish, while growth was compromised in maturing WT males. We demonstrate for the first time in a vertebrate that germ cells are required for pubertal activation of the somatic steroidogenic cells. Text Atlantic salmon PubMed Central (PMC) Scientific Reports 7 1 |
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Article Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B. Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W. Wargelius, Anna Sex steroid production associated with puberty is absent in germ cell-free salmon |
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In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To explore if the absence of germ cells affects puberty or growth in Atlantic salmon, germ cell-free (GCF), dnd knockout and wild type (WT) postsmolts were stimulated to enter puberty. No GCF fish entered puberty, whereas 66.7% (males) and 30% (females) WT fish completed or entered puberty, respectively. Expression of genes related to steroidogenesis (star, cyp17a1, cyp11β, cyp19a1a), gonadal somatic cells (insl3, amh, igf3), oocytes (bmp15), gonadotropin receptors (fshr, lhcgr), and pituitary gonadotropic cells (fshb, lhb, gnrhr4) showed an immature status and failure to up-regulate gonadal sex steroid production in male and female GCF fish was also reflected in low or undetectable plasma sex steroids (11-ketotestosterone, estradiol-17β and testosterone). A gender difference (high in females, low in males) was found in the expression of star and cyp17a1 in GCF fish. No clear difference in growth was detected between GCF and immature WT fish, while growth was compromised in maturing WT males. We demonstrate for the first time in a vertebrate that germ cells are required for pubertal activation of the somatic steroidogenic cells. |
format |
Text |
author |
Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B. Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W. Wargelius, Anna |
author_facet |
Kleppe, Lene Andersson, Eva Skaftnesmo, Kai Ove Edvardsen, Rolf B. Fjelldal, Per Gunnar Norberg, Birgitta Bogerd, Jan Schulz, Rüdiger W. Wargelius, Anna |
author_sort |
Kleppe, Lene |
title |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_short |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_full |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_fullStr |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_full_unstemmed |
Sex steroid production associated with puberty is absent in germ cell-free salmon |
title_sort |
sex steroid production associated with puberty is absent in germ cell-free salmon |
publisher |
Nature Publishing Group UK |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626747/ http://www.ncbi.nlm.nih.gov/pubmed/28974703 https://doi.org/10.1038/s41598-017-12936-w |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5626747/ http://www.ncbi.nlm.nih.gov/pubmed/28974703 http://dx.doi.org/10.1038/s41598-017-12936-w |
op_rights |
© The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
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CC-BY |
op_doi |
https://doi.org/10.1038/s41598-017-12936-w |
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Scientific Reports |
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7 |
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1 |
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1766362983726841856 |