Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water
Ocean acidification is the increase in seawater pCO 2 due to the uptake of atmospheric anthropogenic CO 2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO 2, and associated decrease in pH, represents a climate change‐related stressor. In...
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ftpubmed:oai:pubmedcentral.nih.gov:5606855 2023-05-15T14:30:33+02:00 Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water Bailey, Allison De Wit, Pierre Thor, Peter Browman, Howard I. Bjelland, Reidun Shema, Steven Fields, David M. Runge, Jeffrey A. Thompson, Cameron Hop, Haakon 2017-08-02 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5606855/ http://www.ncbi.nlm.nih.gov/pubmed/28944006 https://doi.org/10.1002/ece3.3063 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5606855/ http://www.ncbi.nlm.nih.gov/pubmed/28944006 http://dx.doi.org/10.1002/ece3.3063 © 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Original Research Text 2017 ftpubmed https://doi.org/10.1002/ece3.3063 2017-10-01T00:09:25Z Ocean acidification is the increase in seawater pCO 2 due to the uptake of atmospheric anthropogenic CO 2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO 2, and associated decrease in pH, represents a climate change‐related stressor. In this study, we investigated the gene expression patterns of nauplii of the Arctic copepod Calanus glacialis cultured at low pH levels. We have previously shown that organismal‐level performance (development, growth, respiration) of C. glacialis nauplii is unaffected by low pH. Here, we investigated the molecular‐level response to lowered pH in order to elucidate the physiological processes involved in this tolerance. Nauplii from wild‐caught C. glacialis were cultured at four pH levels (8.05, 7.9, 7.7, 7.5). At stage N6, mRNA was extracted and sequenced using RNA‐seq. The physiological functionality of the proteins identified was categorized using Gene Ontology and KEGG pathways. We found that the expression of 151 contigs varied significantly with pH on a continuous scale (93% downregulated with decreasing pH). Gene set enrichment analysis revealed that, of the processes downregulated, many were components of the universal cellular stress response, including DNA repair, redox regulation, protein folding, and proteolysis. Sodium:proton antiporters were among the processes significantly upregulated, indicating that these ion pumps were involved in maintaining cellular pH homeostasis. C. glacialis significantly alters its gene expression at low pH, although they maintain normal larval development. Understanding what confers tolerance to some species will support our ability to predict the effects of future ocean acidification on marine organisms. Text Arctic copepod Arctic Calanus glacialis Climate change Ocean acidification PubMed Central (PMC) Arctic Ecology and Evolution 7 18 7145 7160 |
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Original Research Bailey, Allison De Wit, Pierre Thor, Peter Browman, Howard I. Bjelland, Reidun Shema, Steven Fields, David M. Runge, Jeffrey A. Thompson, Cameron Hop, Haakon Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water |
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Original Research |
description |
Ocean acidification is the increase in seawater pCO 2 due to the uptake of atmospheric anthropogenic CO 2, with the largest changes predicted to occur in the Arctic seas. For some marine organisms, this change in pCO 2, and associated decrease in pH, represents a climate change‐related stressor. In this study, we investigated the gene expression patterns of nauplii of the Arctic copepod Calanus glacialis cultured at low pH levels. We have previously shown that organismal‐level performance (development, growth, respiration) of C. glacialis nauplii is unaffected by low pH. Here, we investigated the molecular‐level response to lowered pH in order to elucidate the physiological processes involved in this tolerance. Nauplii from wild‐caught C. glacialis were cultured at four pH levels (8.05, 7.9, 7.7, 7.5). At stage N6, mRNA was extracted and sequenced using RNA‐seq. The physiological functionality of the proteins identified was categorized using Gene Ontology and KEGG pathways. We found that the expression of 151 contigs varied significantly with pH on a continuous scale (93% downregulated with decreasing pH). Gene set enrichment analysis revealed that, of the processes downregulated, many were components of the universal cellular stress response, including DNA repair, redox regulation, protein folding, and proteolysis. Sodium:proton antiporters were among the processes significantly upregulated, indicating that these ion pumps were involved in maintaining cellular pH homeostasis. C. glacialis significantly alters its gene expression at low pH, although they maintain normal larval development. Understanding what confers tolerance to some species will support our ability to predict the effects of future ocean acidification on marine organisms. |
format |
Text |
author |
Bailey, Allison De Wit, Pierre Thor, Peter Browman, Howard I. Bjelland, Reidun Shema, Steven Fields, David M. Runge, Jeffrey A. Thompson, Cameron Hop, Haakon |
author_facet |
Bailey, Allison De Wit, Pierre Thor, Peter Browman, Howard I. Bjelland, Reidun Shema, Steven Fields, David M. Runge, Jeffrey A. Thompson, Cameron Hop, Haakon |
author_sort |
Bailey, Allison |
title |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water |
title_short |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water |
title_full |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water |
title_fullStr |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water |
title_full_unstemmed |
Regulation of gene expression is associated with tolerance of the Arctic copepod Calanus glacialis to CO 2‐acidified sea water |
title_sort |
regulation of gene expression is associated with tolerance of the arctic copepod calanus glacialis to co 2‐acidified sea water |
publisher |
John Wiley and Sons Inc. |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5606855/ http://www.ncbi.nlm.nih.gov/pubmed/28944006 https://doi.org/10.1002/ece3.3063 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic copepod Arctic Calanus glacialis Climate change Ocean acidification |
genre_facet |
Arctic copepod Arctic Calanus glacialis Climate change Ocean acidification |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5606855/ http://www.ncbi.nlm.nih.gov/pubmed/28944006 http://dx.doi.org/10.1002/ece3.3063 |
op_rights |
© 2017 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1002/ece3.3063 |
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Ecology and Evolution |
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7 |
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18 |
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7145 |
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7160 |
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