Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils
Antarctica is an attractive target for human exploration and scientific investigation, however the negative effects of human activity on this continent are long lasting and can have serious consequences on the native ecosystem. Various areas of Antarctica have been contaminated with diesel fuel, whi...
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ftpubmed:oai:pubmedcentral.nih.gov:5581505 2023-05-15T14:03:51+02:00 Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils Gran-Scheuch, Alejandro Fuentes, Edwar Bravo, Denisse M. Jiménez, Juan Cristobal Pérez-Donoso, José M. 2017-08-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5581505/ http://www.ncbi.nlm.nih.gov/pubmed/28894442 https://doi.org/10.3389/fmicb.2017.01634 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5581505/ http://www.ncbi.nlm.nih.gov/pubmed/28894442 http://dx.doi.org/10.3389/fmicb.2017.01634 Copyright © 2017 Gran-Scheuch, Fuentes, Bravo, Jiménez and Pérez-Donoso. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Microbiology Text 2017 ftpubmed https://doi.org/10.3389/fmicb.2017.01634 2017-09-17T01:15:35Z Antarctica is an attractive target for human exploration and scientific investigation, however the negative effects of human activity on this continent are long lasting and can have serious consequences on the native ecosystem. Various areas of Antarctica have been contaminated with diesel fuel, which contains harmful compounds such as heavy metals and polycyclic aromatic hydrocarbons (PAH). Bioremediation of PAHs by the activity of microorganisms is an ecological, economical, and safe decontamination approach. Since the introduction of foreign organisms into the Antarctica is prohibited, it is key to discover native bacteria that can be used for diesel bioremediation. By following the degradation of the PAH phenanthrene, we isolated 53 PAH metabolizing bacteria from diesel contaminated Antarctic soil samples, with three of these isolates exhibiting a high phenanthrene degrading capacity. In particular, the Sphingobium xenophagum D43FB isolate showed the highest phenanthrene degradation ability, generating up to 95% degradation of initial phenanthrene. D43FB can also degrade phenanthrene in the presence of its usual co-pollutant, the heavy metal cadmium, and showed the ability to grow using diesel-fuel as a sole carbon source. Microtiter plate assays and SEM analysis revealed that S. xenophagum D43FB exhibits the ability to form biofilms and can directly adhere to phenanthrene crystals. Genome sequencing analysis also revealed the presence of several genes involved in PAH degradation and heavy metal resistance in the D43FB genome. Altogether, these results demonstrate that S. xenophagum D43FB shows promising potential for its application in the bioremediation of diesel fuel contaminated-Antarctic ecosystems. Text Antarc* Antarctic Antarctica PubMed Central (PMC) Antarctic Frontiers in Microbiology 8 |
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Microbiology |
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Microbiology Gran-Scheuch, Alejandro Fuentes, Edwar Bravo, Denisse M. Jiménez, Juan Cristobal Pérez-Donoso, José M. Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils |
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Microbiology |
description |
Antarctica is an attractive target for human exploration and scientific investigation, however the negative effects of human activity on this continent are long lasting and can have serious consequences on the native ecosystem. Various areas of Antarctica have been contaminated with diesel fuel, which contains harmful compounds such as heavy metals and polycyclic aromatic hydrocarbons (PAH). Bioremediation of PAHs by the activity of microorganisms is an ecological, economical, and safe decontamination approach. Since the introduction of foreign organisms into the Antarctica is prohibited, it is key to discover native bacteria that can be used for diesel bioremediation. By following the degradation of the PAH phenanthrene, we isolated 53 PAH metabolizing bacteria from diesel contaminated Antarctic soil samples, with three of these isolates exhibiting a high phenanthrene degrading capacity. In particular, the Sphingobium xenophagum D43FB isolate showed the highest phenanthrene degradation ability, generating up to 95% degradation of initial phenanthrene. D43FB can also degrade phenanthrene in the presence of its usual co-pollutant, the heavy metal cadmium, and showed the ability to grow using diesel-fuel as a sole carbon source. Microtiter plate assays and SEM analysis revealed that S. xenophagum D43FB exhibits the ability to form biofilms and can directly adhere to phenanthrene crystals. Genome sequencing analysis also revealed the presence of several genes involved in PAH degradation and heavy metal resistance in the D43FB genome. Altogether, these results demonstrate that S. xenophagum D43FB shows promising potential for its application in the bioremediation of diesel fuel contaminated-Antarctic ecosystems. |
format |
Text |
author |
Gran-Scheuch, Alejandro Fuentes, Edwar Bravo, Denisse M. Jiménez, Juan Cristobal Pérez-Donoso, José M. |
author_facet |
Gran-Scheuch, Alejandro Fuentes, Edwar Bravo, Denisse M. Jiménez, Juan Cristobal Pérez-Donoso, José M. |
author_sort |
Gran-Scheuch, Alejandro |
title |
Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils |
title_short |
Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils |
title_full |
Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils |
title_fullStr |
Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils |
title_full_unstemmed |
Isolation and Characterization of Phenanthrene Degrading Bacteria from Diesel Fuel-Contaminated Antarctic Soils |
title_sort |
isolation and characterization of phenanthrene degrading bacteria from diesel fuel-contaminated antarctic soils |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5581505/ http://www.ncbi.nlm.nih.gov/pubmed/28894442 https://doi.org/10.3389/fmicb.2017.01634 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
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Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5581505/ http://www.ncbi.nlm.nih.gov/pubmed/28894442 http://dx.doi.org/10.3389/fmicb.2017.01634 |
op_rights |
Copyright © 2017 Gran-Scheuch, Fuentes, Bravo, Jiménez and Pérez-Donoso. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2017.01634 |
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Frontiers in Microbiology |
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8 |
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1766274700102598656 |