Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa
We assessed the immunomodulatory and anti-inflammatory effects of 9,11-dihydrogracilin A (DHG), a molecule derived from the Antarctic marine sponge Dendrilla membranosa. We used in vitro and in vivo approaches to establish DHG properties. Human peripheral blood mononuclear cells (PBMC) and human ker...
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ftpubmed:oai:pubmedcentral.nih.gov:5578033 2023-05-15T13:53:52+02:00 Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa Ciaglia, Elena Malfitano, Anna Maria Laezza, Chiara Fontana, Angelo Nuzzo, Genoveffa Cutignano, Adele Abate, Mario Pelin, Marco Sosa, Silvio Bifulco, Maurizio Gazzerro, Patrizia 2017-07-28 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578033/ http://www.ncbi.nlm.nih.gov/pubmed/28788056 https://doi.org/10.3390/ijms18081643 en eng MDPI http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578033/ http://www.ncbi.nlm.nih.gov/pubmed/28788056 http://dx.doi.org/10.3390/ijms18081643 © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). CC-BY Article Text 2017 ftpubmed https://doi.org/10.3390/ijms18081643 2017-09-10T00:08:45Z We assessed the immunomodulatory and anti-inflammatory effects of 9,11-dihydrogracilin A (DHG), a molecule derived from the Antarctic marine sponge Dendrilla membranosa. We used in vitro and in vivo approaches to establish DHG properties. Human peripheral blood mononuclear cells (PBMC) and human keratinocytes cell line (HaCaT cells) were used as in vitro system, whereas a model of murine cutaneous irritation was adopted for in vivo studies. We observed that DHG reduces dose dependently the proliferative response and viability of mitogen stimulated PBMC. In addition, DHG induces apoptosis as revealed by AnnexinV staining and downregulates the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), signal transducer and activator of transcription (STAT) and extracellular signal–regulated kinase (ERK) at late time points. These effects were accompanied by down-regulation of interleukin 6 (IL-6) production, slight decrease of IL-10 and no inhibition of tumor necrosis factor-alpha (TNF-α) secretion. To assess potential properties of DHG in epidermal inflammation we used HaCaT cells; this compound reduces cell growth, viability and migration. Finally, we adopted for the in vivo study the croton oil-induced ear dermatitis murine model of inflammation. Of note, topical use of DHG significantly decreased mouse ear edema. These results suggest that DHG exerts anti-inflammatory effects and its anti-edema activity in vivo strongly supports its potential therapeutic application in inflammatory cutaneous diseases. Text Antarc* Antarctic PubMed Central (PMC) Antarctic The Antarctic International Journal of Molecular Sciences 18 8 1643 |
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Article Ciaglia, Elena Malfitano, Anna Maria Laezza, Chiara Fontana, Angelo Nuzzo, Genoveffa Cutignano, Adele Abate, Mario Pelin, Marco Sosa, Silvio Bifulco, Maurizio Gazzerro, Patrizia Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa |
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We assessed the immunomodulatory and anti-inflammatory effects of 9,11-dihydrogracilin A (DHG), a molecule derived from the Antarctic marine sponge Dendrilla membranosa. We used in vitro and in vivo approaches to establish DHG properties. Human peripheral blood mononuclear cells (PBMC) and human keratinocytes cell line (HaCaT cells) were used as in vitro system, whereas a model of murine cutaneous irritation was adopted for in vivo studies. We observed that DHG reduces dose dependently the proliferative response and viability of mitogen stimulated PBMC. In addition, DHG induces apoptosis as revealed by AnnexinV staining and downregulates the phosphorylation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), signal transducer and activator of transcription (STAT) and extracellular signal–regulated kinase (ERK) at late time points. These effects were accompanied by down-regulation of interleukin 6 (IL-6) production, slight decrease of IL-10 and no inhibition of tumor necrosis factor-alpha (TNF-α) secretion. To assess potential properties of DHG in epidermal inflammation we used HaCaT cells; this compound reduces cell growth, viability and migration. Finally, we adopted for the in vivo study the croton oil-induced ear dermatitis murine model of inflammation. Of note, topical use of DHG significantly decreased mouse ear edema. These results suggest that DHG exerts anti-inflammatory effects and its anti-edema activity in vivo strongly supports its potential therapeutic application in inflammatory cutaneous diseases. |
format |
Text |
author |
Ciaglia, Elena Malfitano, Anna Maria Laezza, Chiara Fontana, Angelo Nuzzo, Genoveffa Cutignano, Adele Abate, Mario Pelin, Marco Sosa, Silvio Bifulco, Maurizio Gazzerro, Patrizia |
author_facet |
Ciaglia, Elena Malfitano, Anna Maria Laezza, Chiara Fontana, Angelo Nuzzo, Genoveffa Cutignano, Adele Abate, Mario Pelin, Marco Sosa, Silvio Bifulco, Maurizio Gazzerro, Patrizia |
author_sort |
Ciaglia, Elena |
title |
Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa |
title_short |
Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa |
title_full |
Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa |
title_fullStr |
Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa |
title_full_unstemmed |
Immuno-Modulatory and Anti-Inflammatory Effects of Dihydrogracilin A, a Terpene Derived from the Marine Sponge Dendrilla membranosa |
title_sort |
immuno-modulatory and anti-inflammatory effects of dihydrogracilin a, a terpene derived from the marine sponge dendrilla membranosa |
publisher |
MDPI |
publishDate |
2017 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578033/ http://www.ncbi.nlm.nih.gov/pubmed/28788056 https://doi.org/10.3390/ijms18081643 |
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Antarctic The Antarctic |
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Antarctic The Antarctic |
genre |
Antarc* Antarctic |
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Antarc* Antarctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5578033/ http://www.ncbi.nlm.nih.gov/pubmed/28788056 http://dx.doi.org/10.3390/ijms18081643 |
op_rights |
© 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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CC-BY |
op_doi |
https://doi.org/10.3390/ijms18081643 |
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International Journal of Molecular Sciences |
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18 |
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8 |
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1643 |
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1766259324761407488 |