Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila
The negative-geotaxis climbing assay is used to efficiently study aging and neurodegeneration in Drosophila. To make it suitable for large-scale study, a method called the rapid iterative negative geotaxis (RING) assay has been established by simultaneously photographing the climbing of multiple gro...
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ftpubmed:oai:pubmedcentral.nih.gov:5563671 2023-05-15T15:05:40+02:00 Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila Liu, Haiyan Han, Meng Li, Qingyi Zhang, Xiao Wang, Wen-An Huang, Fu-De 2015-06-15 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563671/ http://www.ncbi.nlm.nih.gov/pubmed/26077703 https://doi.org/10.1007/s12264-014-1526-0 en eng Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563671/ http://www.ncbi.nlm.nih.gov/pubmed/26077703 http://dx.doi.org/10.1007/s12264-014-1526-0 © Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2015 Original Article Text 2015 ftpubmed https://doi.org/10.1007/s12264-014-1526-0 2017-08-27T00:10:52Z The negative-geotaxis climbing assay is used to efficiently study aging and neurodegeneration in Drosophila. To make it suitable for large-scale study, a method called the rapid iterative negative geotaxis (RING) assay has been established by simultaneously photographing the climbing of multiple groups of flies when they are manually tapped down in test tubes. Here, we automated the assay by using a well-controlled electric motor to drive the tapping, and a homemade program to analyze the climbing height of flies. Using the automated RING (aRING) assay, we found that the climbing ability of a strain of wild-type flies, males in particular, declined rapidly before day 21 after eclosion, but slowly from day 21 to 35. We also found that the expression of arctic mutant Aβ42 accelerated the age-dependent decline in the climbing ability of flies. Moreover, using aRING, we examined the effect of third chromosome deficiencies on the accelerated locomotor decline in Aβ42-expressing flies, and isolated 7 suppressors and 15 enhancers. Text Arctic PubMed Central (PMC) Arctic Neuroscience Bulletin 31 5 541 549 |
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Original Article Liu, Haiyan Han, Meng Li, Qingyi Zhang, Xiao Wang, Wen-An Huang, Fu-De Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila |
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Original Article |
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The negative-geotaxis climbing assay is used to efficiently study aging and neurodegeneration in Drosophila. To make it suitable for large-scale study, a method called the rapid iterative negative geotaxis (RING) assay has been established by simultaneously photographing the climbing of multiple groups of flies when they are manually tapped down in test tubes. Here, we automated the assay by using a well-controlled electric motor to drive the tapping, and a homemade program to analyze the climbing height of flies. Using the automated RING (aRING) assay, we found that the climbing ability of a strain of wild-type flies, males in particular, declined rapidly before day 21 after eclosion, but slowly from day 21 to 35. We also found that the expression of arctic mutant Aβ42 accelerated the age-dependent decline in the climbing ability of flies. Moreover, using aRING, we examined the effect of third chromosome deficiencies on the accelerated locomotor decline in Aβ42-expressing flies, and isolated 7 suppressors and 15 enhancers. |
format |
Text |
author |
Liu, Haiyan Han, Meng Li, Qingyi Zhang, Xiao Wang, Wen-An Huang, Fu-De |
author_facet |
Liu, Haiyan Han, Meng Li, Qingyi Zhang, Xiao Wang, Wen-An Huang, Fu-De |
author_sort |
Liu, Haiyan |
title |
Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila |
title_short |
Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila |
title_full |
Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila |
title_fullStr |
Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila |
title_full_unstemmed |
Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila |
title_sort |
automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of aβ42-induced locomotor decline in drosophila |
publisher |
Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences |
publishDate |
2015 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563671/ http://www.ncbi.nlm.nih.gov/pubmed/26077703 https://doi.org/10.1007/s12264-014-1526-0 |
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Arctic |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5563671/ http://www.ncbi.nlm.nih.gov/pubmed/26077703 http://dx.doi.org/10.1007/s12264-014-1526-0 |
op_rights |
© Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag Berlin Heidelberg 2015 |
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https://doi.org/10.1007/s12264-014-1526-0 |
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Neuroscience Bulletin |
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