Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3)
While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on‐farm qualitative test and an in‐laboratory quantitative assay for health assessments in aquacult...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516131/ http://www.ncbi.nlm.nih.gov/pubmed/27905123 https://doi.org/10.1111/jfd.12587 |
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ftpubmed:oai:pubmedcentral.nih.gov:5516131 2023-05-15T15:31:25+02:00 Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) Braceland, M Tinsley, J Cockerill, D Bickerdike, R McLoughlin, M F Eckersall, P D 2016-11-30 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516131/ http://www.ncbi.nlm.nih.gov/pubmed/27905123 https://doi.org/10.1111/jfd.12587 en eng John Wiley and Sons Inc. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516131/ http://www.ncbi.nlm.nih.gov/pubmed/27905123 http://dx.doi.org/10.1111/jfd.12587 © 2016 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. CC-BY Original Articles Text 2016 ftpubmed https://doi.org/10.1111/jfd.12587 2017-08-06T00:19:24Z While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on‐farm qualitative test and an in‐laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ340). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ340 in infected fish rose from undetectable to a maximum at 6 weeks post‐infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon. Text Atlantic salmon Salmo salar PubMed Central (PMC) Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Journal of Fish Diseases 40 8 1077 1087 |
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Original Articles Braceland, M Tinsley, J Cockerill, D Bickerdike, R McLoughlin, M F Eckersall, P D Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) |
topic_facet |
Original Articles |
description |
While investigating biomarkers for infection with salmonid alphavirus (SAV), the cause of pancreas disease (PD), a selective precipitation reaction (SPR) has been discovered in serum which could be an on‐farm qualitative test and an in‐laboratory quantitative assay for health assessments in aquaculture. Mixing serum from Atlantic salmon, Salmo salar, with SAV infection with a sodium acetate buffer caused a visible precipitation which does not occur with serum from healthy salmon. Proteomic examination of the precipitate has revealed that the components are a mix of muscle proteins, for example enolase and aldolase, along with serum protein such as serotransferrin and complement C9. The assay has been optimized for molarity, pH, temperature and wavelength so that the precipitation can be measured as the change in optical density at 340 nm (Δ340). Application of the SPR assay to serum samples from a cohabitation trial of SAV infection in salmon showed that the Δ340 in infected fish rose from undetectable to a maximum at 6 weeks post‐infection correlating with histopathological score of pancreas, heart and muscle damage. This test may have a valuable role to play in the diagnostic evaluation of stock health in salmon. |
format |
Text |
author |
Braceland, M Tinsley, J Cockerill, D Bickerdike, R McLoughlin, M F Eckersall, P D |
author_facet |
Braceland, M Tinsley, J Cockerill, D Bickerdike, R McLoughlin, M F Eckersall, P D |
author_sort |
Braceland, M |
title |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) |
title_short |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) |
title_full |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) |
title_fullStr |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) |
title_full_unstemmed |
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3) |
title_sort |
selective precipitation reaction: a novel diagnostic test for tissue pathology in atlantic salmon, salmo salar, infected with salmonid alphavirus (sav3) |
publisher |
John Wiley and Sons Inc. |
publishDate |
2016 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516131/ http://www.ncbi.nlm.nih.gov/pubmed/27905123 https://doi.org/10.1111/jfd.12587 |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5516131/ http://www.ncbi.nlm.nih.gov/pubmed/27905123 http://dx.doi.org/10.1111/jfd.12587 |
op_rights |
© 2016 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1111/jfd.12587 |
container_title |
Journal of Fish Diseases |
container_volume |
40 |
container_issue |
8 |
container_start_page |
1077 |
op_container_end_page |
1087 |
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1766361912455462912 |